Streptomyces pristinaespiralis (strain ATCC 25486 / DSM 40338 / CBS 914.69 / JCM 4507 / NBRC 13074 / NRRL 2958 / 5647)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; Streptomyces pristinaespiralis

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6866 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|B5H8Q9|B5H8Q9_STRE2 Uncharacterized protein OS=Streptomyces pristinaespiralis (strain ATCC 25486 / DSM 40338 / CBS 914.69 / JCM 4507 / NBRC 13074 / NRRL 2958 / 5647) OX=457429 GN=SSDG_01631 PE=4 SV=1
MM1 pKa = 7.17TVQHH5 pKa = 6.14EE6 pKa = 4.66VPTDD10 pKa = 3.42GATDD14 pKa = 3.67ALEE17 pKa = 3.87VWIDD21 pKa = 3.4QDD23 pKa = 3.99LCTGDD28 pKa = 5.01GICAQYY34 pKa = 10.9APEE37 pKa = 4.23VFEE40 pKa = 5.9LDD42 pKa = 3.05IDD44 pKa = 3.63GLAYY48 pKa = 10.42VKK50 pKa = 10.8SADD53 pKa = 4.84DD54 pKa = 4.41EE55 pKa = 4.67LLQAPGATTPVPLPLLNDD73 pKa = 3.56VVDD76 pKa = 4.2SAKK79 pKa = 10.28EE80 pKa = 3.92CPGDD84 pKa = 4.18CIHH87 pKa = 6.58VRR89 pKa = 11.84RR90 pKa = 11.84VSDD93 pKa = 3.44NVEE96 pKa = 4.07VYY98 pKa = 10.91GPDD101 pKa = 3.4AEE103 pKa = 4.31

Molecular weight:
11.03 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|B5H550|B5H550_STRE2 ECF sigma factor OS=Streptomyces pristinaespiralis (strain ATCC 25486 / DSM 40338 / CBS 914.69 / JCM 4507 / NBRC 13074 / NRRL 2958 / 5647) OX=457429 GN=SSDG_00276 PE=3 SV=1
MM1 pKa = 7.46TFPHH5 pKa = 7.14PGPHH9 pKa = 6.08VGLAGVRR16 pKa = 11.84GSGRR20 pKa = 11.84PGPHH24 pKa = 6.75PNATTRR30 pKa = 11.84FSTLRR35 pKa = 11.84IRR37 pKa = 11.84RR38 pKa = 11.84PRR40 pKa = 11.84TTLRR44 pKa = 11.84TALLRR49 pKa = 11.84GSGTPPGSPSTVRR62 pKa = 11.84RR63 pKa = 11.84RR64 pKa = 11.84RR65 pKa = 11.84LKK67 pKa = 10.57

Molecular weight:
7.26 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6866

0

6866

2152991

25

3624

313.6

33.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.265 ± 0.041

0.827 ± 0.008

6.016 ± 0.022

5.787 ± 0.028

2.787 ± 0.018

9.616 ± 0.027

2.318 ± 0.015

3.202 ± 0.019

2.248 ± 0.029

10.028 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.858 ± 0.012

1.815 ± 0.016

6.09 ± 0.029

2.734 ± 0.015

8.167 ± 0.037

5.118 ± 0.021

6.106 ± 0.028

8.409 ± 0.029

1.519 ± 0.011

2.087 ± 0.017

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski