Castellaniella defragrans 65Phen

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Alcaligenaceae; Castellaniella; Castellaniella defragrans

Average proteome isoelectric point is 6.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3575 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W8X940|W8X940_CASDE Protocatechuate 4 5-dioxygenase beta chain OS=Castellaniella defragrans 65Phen OX=1437824 GN=BN940_08486 PE=4 SV=1
MM1 pKa = 7.88ALPQWTDD8 pKa = 3.16QQVFDD13 pKa = 4.19QMNSGSTWTSSLITYY28 pKa = 8.16AFPQSSSVFDD38 pKa = 4.29PDD40 pKa = 3.99LAEE43 pKa = 4.41HH44 pKa = 6.56FAGFSPLNTAQQPLVHH60 pKa = 6.77LAMMLWDD67 pKa = 4.6DD68 pKa = 5.44LIARR72 pKa = 11.84DD73 pKa = 3.66IVQGSVHH80 pKa = 6.96DD81 pKa = 4.36ADD83 pKa = 4.2IMLSNTSSTDD93 pKa = 2.92GYY95 pKa = 11.33AFAVSGGTVWFSSKK109 pKa = 10.59EE110 pKa = 3.94DD111 pKa = 3.68LLQSPEE117 pKa = 3.64IGKK120 pKa = 10.17DD121 pKa = 3.37GFVTFLHH128 pKa = 6.83EE129 pKa = 4.39IGHH132 pKa = 6.39ALGLNHH138 pKa = 6.48MGDD141 pKa = 4.18YY142 pKa = 10.85NGEE145 pKa = 4.2DD146 pKa = 3.78DD147 pKa = 5.12NGPSSYY153 pKa = 10.46QDD155 pKa = 2.99SDD157 pKa = 3.5MLSIMSYY164 pKa = 10.32YY165 pKa = 10.03GPGMNGGKK173 pKa = 10.41GLVAWGDD180 pKa = 3.45WFASDD185 pKa = 4.4IGSEE189 pKa = 4.8GYY191 pKa = 10.2SPQTPMVNDD200 pKa = 3.14IMVIQRR206 pKa = 11.84LYY208 pKa = 11.16GADD211 pKa = 3.04TTTRR215 pKa = 11.84EE216 pKa = 4.07GNTVYY221 pKa = 10.85GFGSNIQGPLAQIYY235 pKa = 10.15DD236 pKa = 3.77FSINQHH242 pKa = 6.97PILTIYY248 pKa = 10.45DD249 pKa = 3.53AGGVDD254 pKa = 4.69TINLSGWGTDD264 pKa = 3.54SLIDD268 pKa = 4.41LNPGQYY274 pKa = 10.6SSVNGMTNNLAIAKK288 pKa = 7.64ATLIEE293 pKa = 4.12NAVAGAGNDD302 pKa = 3.56VLIGNSADD310 pKa = 3.19NHH312 pKa = 6.45LDD314 pKa = 3.24GGAGQDD320 pKa = 3.0SAMFSGALPGYY331 pKa = 9.59DD332 pKa = 3.23LSYY335 pKa = 11.27DD336 pKa = 3.71VFSRR340 pKa = 11.84EE341 pKa = 3.86YY342 pKa = 10.23TVVDD346 pKa = 3.52MTAGRR351 pKa = 11.84DD352 pKa = 3.82GTDD355 pKa = 2.87TLINIEE361 pKa = 4.03YY362 pKa = 10.77ANFNGAGGDD371 pKa = 4.07LNDD374 pKa = 4.55LTPAVYY380 pKa = 10.18RR381 pKa = 11.84FYY383 pKa = 11.29NAGLGLHH390 pKa = 7.23FYY392 pKa = 10.05TSNNDD397 pKa = 2.62EE398 pKa = 4.2ATAVTRR404 pKa = 11.84MNGFVYY410 pKa = 10.24EE411 pKa = 4.23GVGFGRR417 pKa = 11.84SVEE420 pKa = 4.25GAGIPDD426 pKa = 4.49ADD428 pKa = 3.74TVAVQRR434 pKa = 11.84FYY436 pKa = 11.68NQATGDD442 pKa = 3.54HH443 pKa = 7.26FYY445 pKa = 10.41TAGADD450 pKa = 3.8EE451 pKa = 4.87ARR453 pKa = 11.84HH454 pKa = 5.21LMEE457 pKa = 6.67IGGAWQYY464 pKa = 10.88EE465 pKa = 3.92GRR467 pKa = 11.84AFNAYY472 pKa = 7.09GTQADD477 pKa = 4.43GTTALYY483 pKa = 10.59RR484 pKa = 11.84FLNTEE489 pKa = 4.18SGTHH493 pKa = 7.12FYY495 pKa = 9.87TADD498 pKa = 3.3IAEE501 pKa = 4.83KK502 pKa = 9.53EE503 pKa = 4.2AVMQSGYY510 pKa = 10.66FSYY513 pKa = 10.74EE514 pKa = 3.59GIAFYY519 pKa = 9.76VTAA522 pKa = 5.13

Molecular weight: 56.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W8X0C6|W8X0C6_CASDE DNA polymerase IV OS=Castellaniella defragrans 65Phen OX=1437824 GN=dinB PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVTRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.0RR14 pKa = 11.84THH16 pKa = 5.76GFRR19 pKa = 11.84VRR21 pKa = 11.84MKK23 pKa = 8.99TRR25 pKa = 11.84AGRR28 pKa = 11.84AVLSARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.92GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3575

0

3575

1162262

37

1991

325.1

35.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.341 ± 0.064

0.992 ± 0.015

5.632 ± 0.029

5.097 ± 0.037

3.327 ± 0.028

8.823 ± 0.042

2.317 ± 0.021

4.482 ± 0.031

2.33 ± 0.03

11.124 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.381 ± 0.019

2.168 ± 0.023

5.583 ± 0.036

3.756 ± 0.029

8.099 ± 0.047

4.918 ± 0.031

4.661 ± 0.028

7.172 ± 0.033

1.526 ± 0.018

2.272 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski