Scleropages formosus (Asian bonytongue) (Osteoglossum formosum)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Actinopterygii; Actinopteri; Neopterygii; Teleostei; Osteoglossocephalai; Osteoglossocephala;

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 24200 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0P7XQ50|A0A0P7XQ50_SCLFO Uncharacterized protein OS=Scleropages formosus OX=113540 GN=Z043_101413 PE=4 SV=1
II1 pKa = 7.96KK2 pKa = 10.27EE3 pKa = 4.1IIFLKK8 pKa = 8.52NTIMEE13 pKa = 4.42CEE15 pKa = 4.03ACGMGNDD22 pKa = 4.29KK23 pKa = 8.76PTHH26 pKa = 6.1PSCVPNPCHH35 pKa = 6.51PGVMCMEE42 pKa = 4.19TAGGIKK48 pKa = 10.08CGPCPEE54 pKa = 4.18GTEE57 pKa = 4.59GNGIHH62 pKa = 6.17CTDD65 pKa = 3.76VDD67 pKa = 3.73EE68 pKa = 5.84CLTKK72 pKa = 10.33PCHH75 pKa = 6.12TGVRR79 pKa = 11.84CINTFPGFRR88 pKa = 11.84CGPCPAGFTGPVVQGIGLSYY108 pKa = 11.24ARR110 pKa = 11.84TNKK113 pKa = 9.79QGSFHH118 pKa = 7.18CGPCKK123 pKa = 10.07PGFIGDD129 pKa = 3.6QQRR132 pKa = 11.84GCKK135 pKa = 9.66AEE137 pKa = 4.19KK138 pKa = 10.71ACGTDD143 pKa = 3.76QLNLCHH149 pKa = 6.56TNAEE153 pKa = 4.73CIVHH157 pKa = 6.84RR158 pKa = 11.84DD159 pKa = 3.33GSIEE163 pKa = 4.27CVCGVGWAGNGYY175 pKa = 9.93LCGLDD180 pKa = 3.18IDD182 pKa = 4.57IDD184 pKa = 3.96GFPDD188 pKa = 3.28EE189 pKa = 5.37KK190 pKa = 11.21LNCPEE195 pKa = 4.13TNCAKK200 pKa = 10.52DD201 pKa = 3.25NCLTVPNSGQEE212 pKa = 3.86DD213 pKa = 4.02ADD215 pKa = 4.12RR216 pKa = 11.84DD217 pKa = 4.5GIGDD221 pKa = 4.0ACDD224 pKa = 3.53EE225 pKa = 4.74DD226 pKa = 5.68ADD228 pKa = 4.2GDD230 pKa = 4.54GIINTEE236 pKa = 4.36DD237 pKa = 3.41NCVLKK242 pKa = 10.98PNVNQRR248 pKa = 11.84NVDD251 pKa = 3.21QDD253 pKa = 4.02DD254 pKa = 4.43FGDD257 pKa = 3.82ACDD260 pKa = 3.59NCRR263 pKa = 11.84LIINNDD269 pKa = 3.34QKK271 pKa = 11.27DD272 pKa = 3.3TDD274 pKa = 4.15ADD276 pKa = 4.15SKK278 pKa = 11.83GDD280 pKa = 3.68ACDD283 pKa = 4.97DD284 pKa = 5.26DD285 pKa = 5.19IDD287 pKa = 6.24GDD289 pKa = 4.41GIPNNVDD296 pKa = 2.97NCVMVPNRR304 pKa = 11.84DD305 pKa = 3.32QKK307 pKa = 11.54DD308 pKa = 3.36RR309 pKa = 11.84DD310 pKa = 3.49GDD312 pKa = 4.3GVGDD316 pKa = 4.14ACDD319 pKa = 3.15SCPYY323 pKa = 9.76VRR325 pKa = 11.84NPDD328 pKa = 3.61QLDD331 pKa = 3.29VDD333 pKa = 4.07NDD335 pKa = 4.78LIGDD339 pKa = 3.96PCDD342 pKa = 3.62TNKK345 pKa = 10.93DD346 pKa = 3.49SDD348 pKa = 4.16GDD350 pKa = 3.81GHH352 pKa = 6.91QDD354 pKa = 4.2SRR356 pKa = 11.84DD357 pKa = 3.47NCPAVINSSQLDD369 pKa = 3.54TDD371 pKa = 3.76RR372 pKa = 11.84DD373 pKa = 4.22GVGDD377 pKa = 4.09EE378 pKa = 5.64CDD380 pKa = 5.36DD381 pKa = 5.71DD382 pKa = 6.34DD383 pKa = 7.03DD384 pKa = 6.2NDD386 pKa = 5.25GIPDD390 pKa = 4.77LFPPGPDD397 pKa = 2.86NCRR400 pKa = 11.84LVPNPLQEE408 pKa = 5.49DD409 pKa = 3.7SDD411 pKa = 4.43GDD413 pKa = 4.19GIGNVCEE420 pKa = 4.02NDD422 pKa = 3.15YY423 pKa = 11.84DD424 pKa = 4.22NDD426 pKa = 4.18TFVDD430 pKa = 4.53MIDD433 pKa = 3.5VCPEE437 pKa = 3.68NAEE440 pKa = 4.04VTLTDD445 pKa = 3.96FRR447 pKa = 11.84AFQTVVLDD455 pKa = 4.1PEE457 pKa = 5.32GDD459 pKa = 3.65AQIDD463 pKa = 4.13PNWVVLNQGRR473 pKa = 11.84EE474 pKa = 3.9IVQTMNSDD482 pKa = 3.19PGLAVGYY489 pKa = 7.31TAFNGVDD496 pKa = 3.99FEE498 pKa = 4.97GTFHH502 pKa = 6.81VNTVTDD508 pKa = 3.57DD509 pKa = 4.08DD510 pKa = 4.35YY511 pKa = 12.0AGFIFGYY518 pKa = 9.58QDD520 pKa = 2.83SSSFYY525 pKa = 9.91VVMWKK530 pKa = 9.52QVEE533 pKa = 4.18QIYY536 pKa = 8.33WQANPFRR543 pKa = 11.84AVAEE547 pKa = 4.08PGIQLKK553 pKa = 10.06AVKK556 pKa = 10.35SNTGPGEE563 pKa = 3.91NLRR566 pKa = 11.84NSLWHH571 pKa = 6.45TGDD574 pKa = 3.39SNGQVRR580 pKa = 11.84LLWKK584 pKa = 10.35DD585 pKa = 3.22PRR587 pKa = 11.84NVGWKK592 pKa = 10.34DD593 pKa = 3.02KK594 pKa = 10.83TSYY597 pKa = 10.52RR598 pKa = 11.84WFLQHH603 pKa = 6.88RR604 pKa = 11.84PHH606 pKa = 7.4DD607 pKa = 4.02GYY609 pKa = 10.74IRR611 pKa = 11.84VRR613 pKa = 11.84FYY615 pKa = 11.04EE616 pKa = 4.86GPQMVADD623 pKa = 3.92SGIIIDD629 pKa = 3.57TTMRR633 pKa = 11.84GGRR636 pKa = 11.84LGVFCFSQEE645 pKa = 3.94NIIWSNLRR653 pKa = 11.84YY654 pKa = 9.68RR655 pKa = 11.84CNDD658 pKa = 3.2TTPEE662 pKa = 4.15DD663 pKa = 3.77FDD665 pKa = 3.94TYY667 pKa = 10.59RR668 pKa = 11.84IQQIQLQAA676 pKa = 3.49

Molecular weight: 73.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0P7Y0A6|A0A0P7Y0A6_SCLFO KN motif and ankyrin repeat domain-containing protein 2-like (Fragment) OS=Scleropages formosus OX=113540 GN=Z043_123050 PE=4 SV=1
MM1 pKa = 7.49PRR3 pKa = 11.84SFMWAPDD10 pKa = 3.59PQAWLRR16 pKa = 11.84DD17 pKa = 3.89DD18 pKa = 4.78AVPTALAVLWAAMALEE34 pKa = 4.71GRR36 pKa = 11.84CGEE39 pKa = 4.42RR40 pKa = 11.84GATPARR46 pKa = 11.84RR47 pKa = 11.84GRR49 pKa = 11.84RR50 pKa = 11.84RR51 pKa = 11.84RR52 pKa = 11.84QAPVPRR58 pKa = 11.84TRR60 pKa = 11.84LHH62 pKa = 6.47GLRR65 pKa = 11.84HH66 pKa = 4.37VCARR70 pKa = 11.84GSSIGRR76 pKa = 11.84RR77 pKa = 11.84TFWILALCTSLGLLLSWSSNRR98 pKa = 11.84LLHH101 pKa = 6.06WLSFPTYY108 pKa = 8.99TKK110 pKa = 10.87VHH112 pKa = 5.47TEE114 pKa = 3.7WAKK117 pKa = 10.13EE118 pKa = 3.91LPFPTVTICNNNPVRR133 pKa = 11.84FYY135 pKa = 11.58KK136 pKa = 9.61LTKK139 pKa = 9.75SDD141 pKa = 4.41LYY143 pKa = 11.08FAGHH147 pKa = 6.37WLGLLLANRR156 pKa = 11.84TARR159 pKa = 11.84PLVLDD164 pKa = 4.46LLQEE168 pKa = 4.02DD169 pKa = 3.71RR170 pKa = 11.84RR171 pKa = 11.84AWFHH175 pKa = 6.32KK176 pKa = 10.68LSDD179 pKa = 3.79FRR181 pKa = 11.84LFLPPRR187 pKa = 11.84HH188 pKa = 6.13FEE190 pKa = 4.03GTNLEE195 pKa = 4.16FMDD198 pKa = 4.46RR199 pKa = 11.84LSHH202 pKa = 6.68QLDD205 pKa = 4.24DD206 pKa = 4.07MLLSCKK212 pKa = 10.06YY213 pKa = 9.52RR214 pKa = 11.84GEE216 pKa = 4.02MCGPQNFSTATRR228 pKa = 11.84GRR230 pKa = 11.84VAAGNKK236 pKa = 9.94DD237 pKa = 3.58FGPPKK242 pKa = 9.76EE243 pKa = 4.17KK244 pKa = 10.68SVTHH248 pKa = 7.09GITGGIDD255 pKa = 3.25LQALSNGTGAIWMGKK270 pKa = 8.83GPQGEE275 pKa = 4.69GPSSNITGAYY285 pKa = 9.27IIMLPGPGFNGPMISLEE302 pKa = 4.06TTNNHH307 pKa = 5.23RR308 pKa = 11.84VWVFRR313 pKa = 11.84DD314 pKa = 3.47PGRR317 pKa = 11.84NRR319 pKa = 11.84QGQSSRR325 pKa = 3.48

Molecular weight: 36.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

24199

1

24200

11792707

31

19435

487.3

54.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.942 ± 0.014

2.344 ± 0.014

5.011 ± 0.011

6.849 ± 0.019

3.625 ± 0.012

6.582 ± 0.02

2.612 ± 0.009

4.149 ± 0.014

5.368 ± 0.02

9.697 ± 0.022

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.333 ± 0.007

3.535 ± 0.01

5.865 ± 0.022

4.611 ± 0.015

6.007 ± 0.014

8.538 ± 0.021

5.382 ± 0.013

6.555 ± 0.014

1.213 ± 0.006

2.702 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski