Lysobacter sp. Root96

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales; Xanthomonadaceae; Lysobacter; unclassified Lysobacter

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3827 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q9ERP3|A0A0Q9ERP3_9GAMM Phage_sheath_1 domain-containing protein OS=Lysobacter sp. Root96 OX=1736612 GN=ASE45_05395 PE=3 SV=1
MM1 pKa = 7.52AKK3 pKa = 10.1SVDD6 pKa = 3.48NANPVVGTNVVFTLTVTNNGPSASAGVSVADD37 pKa = 4.76LLPNGYY43 pKa = 7.98TYY45 pKa = 11.31VSDD48 pKa = 3.95NSAGAYY54 pKa = 9.87VPGTGVWTIGSLANAASTSLQITATVNATGTYY86 pKa = 10.29LNTATVSSTTNDD98 pKa = 3.2PTPANNTGTATTTPTASADD117 pKa = 3.59LSIDD121 pKa = 3.47KK122 pKa = 10.47DD123 pKa = 3.73DD124 pKa = 4.86AGASVVPGNNLVYY137 pKa = 10.14TIIVANAGPSAASNVVVSDD156 pKa = 5.14PIPAGLNFVSAAGAGWACVNNAGTVEE182 pKa = 4.25CSRR185 pKa = 11.84PTLAAGSNAAIALTLAVPANYY206 pKa = 10.18AGASSISNTASVGSATADD224 pKa = 3.45PNSGNDD230 pKa = 3.24SDD232 pKa = 5.19TEE234 pKa = 4.28TTPVSISIDD243 pKa = 3.64AVDD246 pKa = 4.53DD247 pKa = 4.08AATVLPFAGGTVPNVTVNDD266 pKa = 3.91TTNGVAVVIGVNATAPSIVSNGGLTGLIANPDD298 pKa = 3.19GSFTVPAGTAPGAYY312 pKa = 7.55TVGYY316 pKa = 9.07RR317 pKa = 11.84ICTLPATTPATCDD330 pKa = 3.5DD331 pKa = 4.16ANVPVVVGPNADD343 pKa = 4.49DD344 pKa = 5.13DD345 pKa = 5.4NEE347 pKa = 4.36TTPQNVALNGNASTNDD363 pKa = 3.34TASAGSVYY371 pKa = 10.93SLVSGPASGTLTFNADD387 pKa = 2.66GSYY390 pKa = 11.13VYY392 pKa = 10.25TPNTNFNGSDD402 pKa = 3.2SFNYY406 pKa = 9.18RR407 pKa = 11.84VCLPAPNGTVCDD419 pKa = 3.72TATVNITITAATIVAVDD436 pKa = 3.93DD437 pKa = 3.83TAATNFNTPVATNAVGNDD455 pKa = 3.49TTSGGVIDD463 pKa = 4.52PASVVATSGANGNVTCAAGSCTYY486 pKa = 10.81SPNPGFAGVDD496 pKa = 3.32SYY498 pKa = 12.07DD499 pKa = 3.55YY500 pKa = 9.36TVCLAAPNGTVCDD513 pKa = 3.67TATVNVVVGPNAGDD527 pKa = 3.82DD528 pKa = 4.75SEE530 pKa = 4.54TTPQNTALNATVTGNDD546 pKa = 3.46SAPAGAAYY554 pKa = 9.19TLVGGTANGTLTFNSDD570 pKa = 2.41GSYY573 pKa = 11.09VYY575 pKa = 10.01TPNANYY581 pKa = 10.44SGPDD585 pKa = 3.04SFNYY589 pKa = 9.64SVCLPAPNGTVCDD602 pKa = 3.87TATVTITVGGNTVTAVDD619 pKa = 5.01DD620 pKa = 4.5SASTPQNTPVGISVIGNDD638 pKa = 3.56TTTGAPLNPASVSVVTPPTSGSVSCDD664 pKa = 3.04SAGLCTYY671 pKa = 9.59TPTAGFSGNTSFVYY685 pKa = 9.83RR686 pKa = 11.84VCDD689 pKa = 3.38VSTPTPVCANATVTITVAQAVIVANDD715 pKa = 3.84DD716 pKa = 4.23DD717 pKa = 5.36YY718 pKa = 12.06SGSPVNGTNGSANVGNALTNDD739 pKa = 3.89SLNGGAIDD747 pKa = 4.06LAFVTLTVNDD757 pKa = 4.73PNPGDD762 pKa = 3.84GVAVASDD769 pKa = 3.65GTVSVAAGTPAGSYY783 pKa = 8.59VIPYY787 pKa = 7.42QLCEE791 pKa = 3.94TLHH794 pKa = 6.85PSNCDD799 pKa = 2.98PATITVVVNAAPIDD813 pKa = 4.0AVDD816 pKa = 4.84DD817 pKa = 4.24PVATPVNGSTGGTGVINVFGNDD839 pKa = 3.66TLNGAAATTTNVVLTPVSNGPLTVNANGTVDD870 pKa = 3.57VAPNTPAGVYY880 pKa = 7.57TVSYY884 pKa = 9.3RR885 pKa = 11.84ICEE888 pKa = 3.95VLNPSNCDD896 pKa = 3.13TALVTVTVNAAPIDD910 pKa = 3.93AVDD913 pKa = 4.58DD914 pKa = 4.27VVATPVNGSTGATAVVNVLGNDD936 pKa = 3.71TLNGSTVNPADD947 pKa = 3.56VTLTPVTSGPLTVNANGTVDD967 pKa = 3.57VAPNTPAGTYY977 pKa = 7.8TVTYY981 pKa = 8.18QICEE985 pKa = 3.89MLNPSNCDD993 pKa = 3.0TAVATVTVVAPAITAVDD1010 pKa = 3.88DD1011 pKa = 4.05PVGGGNGVTPQNTPLVTSVLGNDD1034 pKa = 4.11TLNGAPIDD1042 pKa = 3.69PTLINITIASNPANGTVTVNSNGTIVYY1069 pKa = 8.01TPNPNFSGNDD1079 pKa = 3.09SYY1081 pKa = 11.78TYY1083 pKa = 8.8TICEE1087 pKa = 4.15KK1088 pKa = 10.84LNPANCATATVSVLVQPNLVVATDD1112 pKa = 4.6DD1113 pKa = 3.59VTTTNQQVPVTISVLGNDD1131 pKa = 4.18TVTSAPLDD1139 pKa = 3.82PASVFVTVAPANGTVNCAAGVCTYY1163 pKa = 10.12TPNQFFAGTDD1173 pKa = 3.09SFTYY1177 pKa = 9.8RR1178 pKa = 11.84VCDD1181 pKa = 3.43TSVPTPVCDD1190 pKa = 3.17IAVVTITVAANAPVLRR1206 pKa = 11.84LSKK1209 pKa = 10.6QSAQRR1214 pKa = 11.84SVKK1217 pKa = 10.15IGDD1220 pKa = 3.57LVRR1223 pKa = 11.84YY1224 pKa = 6.96TVIAEE1229 pKa = 4.17NVGDD1233 pKa = 4.08SPARR1237 pKa = 11.84NATLVDD1243 pKa = 3.78TPPAGFTYY1251 pKa = 10.75VDD1253 pKa = 3.8GSLSVDD1259 pKa = 4.31DD1260 pKa = 5.24EE1261 pKa = 6.07DD1262 pKa = 4.94DD1263 pKa = 3.7AASIGGTNPLRR1274 pKa = 11.84INGIDD1279 pKa = 3.39VAIGQRR1285 pKa = 11.84ATIVYY1290 pKa = 8.18FLRR1293 pKa = 11.84VGAGVGKK1300 pKa = 10.03GVHH1303 pKa = 6.21SNSVTALDD1311 pKa = 4.44SNGSSIGNVATADD1324 pKa = 3.09VDD1326 pKa = 3.9MAGDD1330 pKa = 3.84PLLEE1334 pKa = 5.45DD1335 pKa = 3.79SLIMGSVYY1343 pKa = 10.66DD1344 pKa = 5.44DD1345 pKa = 3.81RR1346 pKa = 11.84DD1347 pKa = 3.32GDD1349 pKa = 3.82GWQDD1353 pKa = 3.2PAKK1356 pKa = 10.38AGGVRR1361 pKa = 11.84VQGGFAPGAYY1371 pKa = 9.25VAGSTTVDD1379 pKa = 3.5RR1380 pKa = 11.84GTGPQPEE1387 pKa = 4.96PDD1389 pKa = 3.18TSAPLLHH1396 pKa = 7.09GIAIGDD1402 pKa = 3.46IVGRR1406 pKa = 11.84SSSADD1411 pKa = 3.1DD1412 pKa = 3.82AQRR1415 pKa = 11.84HH1416 pKa = 4.29QVVVRR1421 pKa = 11.84QTLRR1425 pKa = 11.84SLDD1428 pKa = 3.77FSDD1431 pKa = 6.03DD1432 pKa = 3.67FVLTTDD1438 pKa = 3.43EE1439 pKa = 4.55GSSVRR1444 pKa = 11.84MNAAGQTTVDD1454 pKa = 3.71RR1455 pKa = 11.84VRR1457 pKa = 11.84GDD1459 pKa = 3.13AAKK1462 pKa = 10.82GLTAQDD1468 pKa = 3.7LQVTRR1473 pKa = 11.84QVSQVADD1480 pKa = 4.43GYY1482 pKa = 9.76EE1483 pKa = 3.68VSYY1486 pKa = 9.45TIRR1489 pKa = 11.84NEE1491 pKa = 4.49GIDD1494 pKa = 3.51EE1495 pKa = 4.26RR1496 pKa = 11.84GIPGVRR1502 pKa = 11.84IASVEE1507 pKa = 3.95GLIMQTDD1514 pKa = 3.99AYY1516 pKa = 10.35GRR1518 pKa = 11.84YY1519 pKa = 8.65HH1520 pKa = 7.97LEE1522 pKa = 4.71GISGGDD1528 pKa = 3.26AARR1531 pKa = 11.84GRR1533 pKa = 11.84NFILKK1538 pKa = 9.47VDD1540 pKa = 3.99QATLPPGSVFTTEE1553 pKa = 3.64NPRR1556 pKa = 11.84VRR1558 pKa = 11.84RR1559 pKa = 11.84VTQGVPTRR1567 pKa = 11.84FDD1569 pKa = 3.62FGVKK1573 pKa = 10.26LPAGEE1578 pKa = 3.93IRR1580 pKa = 11.84GGSSEE1585 pKa = 4.08TDD1587 pKa = 3.2VEE1589 pKa = 4.31LGEE1592 pKa = 4.39VMFEE1596 pKa = 4.05AGSDD1600 pKa = 3.8QVKK1603 pKa = 10.5SDD1605 pKa = 3.73YY1606 pKa = 11.59APMFGKK1612 pKa = 10.17IAQRR1616 pKa = 11.84LRR1618 pKa = 11.84DD1619 pKa = 3.64ADD1621 pKa = 3.92GGSVTIAAQAEE1632 pKa = 4.25AEE1634 pKa = 4.09ALAFARR1640 pKa = 11.84AHH1642 pKa = 6.13AVRR1645 pKa = 11.84AALSKK1650 pKa = 10.43EE1651 pKa = 4.11LEE1653 pKa = 4.09PALAAKK1659 pKa = 10.11VRR1661 pKa = 11.84IDD1663 pKa = 3.61VVAGTDD1669 pKa = 3.33ASALVSLDD1677 pKa = 3.31QAIKK1681 pKa = 10.6LGEE1684 pKa = 4.22LLFDD1688 pKa = 4.14TDD1690 pKa = 3.15QDD1692 pKa = 4.22TIRR1695 pKa = 11.84PQYY1698 pKa = 10.06RR1699 pKa = 11.84GLISEE1704 pKa = 4.17IAKK1707 pKa = 8.74TLNRR1711 pKa = 11.84EE1712 pKa = 3.63GRR1714 pKa = 11.84GVIGIVGRR1722 pKa = 11.84ADD1724 pKa = 3.21PRR1726 pKa = 11.84GANAYY1731 pKa = 9.23NVQLGLRR1738 pKa = 11.84RR1739 pKa = 11.84AKK1741 pKa = 10.56AVFEE1745 pKa = 4.85AISAEE1750 pKa = 4.12LEE1752 pKa = 4.14PKK1754 pKa = 10.0VKK1756 pKa = 10.48QKK1758 pKa = 10.35VRR1760 pKa = 11.84VDD1762 pKa = 3.04ITDD1765 pKa = 3.48DD1766 pKa = 3.31TNAPVGVGGRR1776 pKa = 3.6

Molecular weight: 178.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q9EMA4|A0A0Q9EMA4_9GAMM Chaperone protein ClpB OS=Lysobacter sp. Root96 OX=1736612 GN=clpB PE=3 SV=1
MM1 pKa = 6.66STGRR5 pKa = 11.84RR6 pKa = 11.84LASRR10 pKa = 11.84AIAWQAGATALTALAFLPLGARR32 pKa = 11.84FALAAAVGGGAVFAGAFVAAWMALGGGVQPAGAAIGRR69 pKa = 11.84LLAGVMFKK77 pKa = 10.19WVVVFLVLGLGLAVFRR93 pKa = 11.84LPPLPMLVGVLAATLAFVLANLLKK117 pKa = 10.59RR118 pKa = 4.01

Molecular weight: 11.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3827

0

3827

1228680

41

2854

321.1

34.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.55 ± 0.056

0.834 ± 0.012

5.931 ± 0.034

5.317 ± 0.038

3.291 ± 0.027

8.629 ± 0.038

2.11 ± 0.019

4.087 ± 0.028

2.72 ± 0.032

10.709 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.033 ± 0.019

2.514 ± 0.031

5.409 ± 0.029

3.641 ± 0.027

7.858 ± 0.041

5.269 ± 0.033

4.788 ± 0.042

7.219 ± 0.037

1.548 ± 0.02

2.541 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski