Lake Sarah-associated circular virus-44

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 8.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A140AQQ9|A0A140AQQ9_9VIRU Replication associated protein OS=Lake Sarah-associated circular virus-44 OX=1685773 PE=4 SV=1
MM1 pKa = 7.53EE2 pKa = 6.25KK3 pKa = 9.62IRR5 pKa = 11.84YY6 pKa = 8.25YY7 pKa = 10.36ILTIPHH13 pKa = 5.85YY14 pKa = 10.38AYY16 pKa = 9.73TPFLHH21 pKa = 6.5TSCNYY26 pKa = 9.59VRR28 pKa = 11.84GQLEE32 pKa = 4.04QGASGYY38 pKa = 8.25LHH40 pKa = 5.98WQMVVFLKK48 pKa = 10.69SPQRR52 pKa = 11.84RR53 pKa = 11.84SFVKK57 pKa = 10.29KK58 pKa = 10.36LYY60 pKa = 10.52GDD62 pKa = 3.69EE63 pKa = 4.09AHH65 pKa = 7.2IEE67 pKa = 4.16PTRR70 pKa = 11.84SAAAMEE76 pKa = 4.42YY77 pKa = 9.72VWKK80 pKa = 10.8QEE82 pKa = 3.77TSVPNTQFEE91 pKa = 4.78LGSLPTSRR99 pKa = 11.84CSSKK103 pKa = 10.83DD104 pKa = 2.42WDD106 pKa = 4.79AIRR109 pKa = 11.84SSAVEE114 pKa = 3.9GRR116 pKa = 11.84MEE118 pKa = 5.09SIPSDD123 pKa = 3.79VYY125 pKa = 10.08IRR127 pKa = 11.84CYY129 pKa = 11.0NSLKK133 pKa = 10.44RR134 pKa = 11.84IAVDD138 pKa = 3.07HH139 pKa = 6.4SKK141 pKa = 10.37PVCIEE146 pKa = 3.65RR147 pKa = 11.84KK148 pKa = 9.19IIVYY152 pKa = 8.85YY153 pKa = 10.86GPTGTGKK160 pKa = 9.96SRR162 pKa = 11.84RR163 pKa = 11.84AWDD166 pKa = 3.3EE167 pKa = 3.98AGFDD171 pKa = 4.8AYY173 pKa = 10.07PKK175 pKa = 10.72DD176 pKa = 3.98PNTKK180 pKa = 9.66FWDD183 pKa = 4.66GYY185 pKa = 9.71NGHH188 pKa = 6.78KK189 pKa = 10.52NVVIDD194 pKa = 3.86EE195 pKa = 4.11FRR197 pKa = 11.84GIINISNVLRR207 pKa = 11.84WFDD210 pKa = 3.77RR211 pKa = 11.84YY212 pKa = 9.66PVLVEE217 pKa = 4.1VKK219 pKa = 10.41GSSAVFKK226 pKa = 11.22AEE228 pKa = 4.29NIWITSNLHH237 pKa = 5.82PDD239 pKa = 2.94DD240 pKa = 4.98WYY242 pKa = 10.7PEE244 pKa = 4.25LDD246 pKa = 3.79PATKK250 pKa = 10.26LALKK254 pKa = 10.35RR255 pKa = 11.84RR256 pKa = 11.84LLIVNILL263 pKa = 3.44

Molecular weight: 30.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A140AQQ9|A0A140AQQ9_9VIRU Replication associated protein OS=Lake Sarah-associated circular virus-44 OX=1685773 PE=4 SV=1
MM1 pKa = 7.9RR2 pKa = 11.84FGWWPHH8 pKa = 4.66HH9 pKa = 6.33HH10 pKa = 7.13CGPGNYY16 pKa = 8.63IKK18 pKa = 10.44SYY20 pKa = 10.76RR21 pKa = 11.84SLHH24 pKa = 6.17DD25 pKa = 3.26HH26 pKa = 7.03TDD28 pKa = 3.77NICRR32 pKa = 11.84DD33 pKa = 2.87HH34 pKa = 7.21DD35 pKa = 3.65KK36 pKa = 11.02AYY38 pKa = 10.72RR39 pKa = 11.84RR40 pKa = 11.84YY41 pKa = 9.88GNKK44 pKa = 10.14AYY46 pKa = 9.62IYY48 pKa = 9.95YY49 pKa = 10.59NKK51 pKa = 10.54ADD53 pKa = 3.5DD54 pKa = 3.81RR55 pKa = 11.84FIRR58 pKa = 11.84RR59 pKa = 11.84MDD61 pKa = 3.1RR62 pKa = 11.84RR63 pKa = 11.84KK64 pKa = 10.4GVWPKK69 pKa = 10.27IYY71 pKa = 10.53AGYY74 pKa = 10.34FKK76 pKa = 10.69GKK78 pKa = 10.01KK79 pKa = 8.87RR80 pKa = 11.84VAPVMDD86 pKa = 4.19EE87 pKa = 3.8PRR89 pKa = 11.84KK90 pKa = 9.48KK91 pKa = 10.34RR92 pKa = 11.84KK93 pKa = 10.14LEE95 pKa = 3.83VSFSSSKK102 pKa = 10.64NDD104 pKa = 3.54EE105 pKa = 4.09NSEE108 pKa = 4.2MPRR111 pKa = 11.84SRR113 pKa = 11.84SRR115 pKa = 11.84GRR117 pKa = 11.84TPTRR121 pKa = 11.84SKK123 pKa = 11.35SPMPTPRR130 pKa = 11.84KK131 pKa = 9.54SRR133 pKa = 11.84SPKK136 pKa = 8.88KK137 pKa = 8.37TYY139 pKa = 9.07QSRR142 pKa = 11.84GTSTDD147 pKa = 3.35SIRR150 pKa = 11.84NRR152 pKa = 11.84SMSSGRR158 pKa = 11.84ASSSKK163 pKa = 9.17ATAVIAASSTDD174 pKa = 3.35VVRR177 pKa = 11.84ARR179 pKa = 11.84TTKK182 pKa = 10.15KK183 pKa = 10.75GRR185 pKa = 11.84ILDD188 pKa = 3.78KK189 pKa = 10.52GTPNLLAGNLVAKK202 pKa = 9.44TDD204 pKa = 3.44KK205 pKa = 10.79KK206 pKa = 9.81FNKK209 pKa = 8.73MSYY212 pKa = 9.34PGWVHH217 pKa = 6.55EE218 pKa = 4.31NVTTGAAVTDD228 pKa = 3.9AQAIIISHH236 pKa = 6.26TDD238 pKa = 2.87MAQRR242 pKa = 11.84NVIQTVARR250 pKa = 11.84ALVKK254 pKa = 10.55SLWRR258 pKa = 11.84KK259 pKa = 8.31CGVYY263 pKa = 8.67VTSLEE268 pKa = 4.19QPIPPAWAVAPFPTARR284 pKa = 11.84LEE286 pKa = 4.09IRR288 pKa = 11.84DD289 pKa = 4.01LVSNGATVAVWTYY302 pKa = 9.1TATALSTFNDD312 pKa = 3.22WCVSLSTSIEE322 pKa = 4.22TYY324 pKa = 10.32LVSNLYY330 pKa = 10.55GSWAPAIMTCSPKK343 pKa = 10.65GNNGLTVEE351 pKa = 4.73FDD353 pKa = 3.32LSKK356 pKa = 11.64AEE358 pKa = 4.16VTVLTSNILKK368 pKa = 10.15VKK370 pKa = 10.15NISVDD375 pKa = 3.4GTNDD379 pKa = 3.67SILDD383 pKa = 3.63VNASPFIGKK392 pKa = 10.04GYY394 pKa = 10.15IIGGTNIEE402 pKa = 4.44FKK404 pKa = 10.52GYY406 pKa = 9.31PYY408 pKa = 10.85VIGGDD413 pKa = 3.94LNFSPDD419 pKa = 3.19TDD421 pKa = 2.87SGYY424 pKa = 8.62TVKK427 pKa = 9.85GTASVGGTKK436 pKa = 8.96IDD438 pKa = 3.8RR439 pKa = 11.84PPLKK443 pKa = 10.03ATIGRR448 pKa = 11.84CRR450 pKa = 11.84SMGNIGMNVGEE461 pKa = 4.69IKK463 pKa = 10.57SHH465 pKa = 5.75IAGYY469 pKa = 7.1SHH471 pKa = 7.56KK472 pKa = 10.89GNLWTFLTKK481 pKa = 10.65LGVRR485 pKa = 11.84SSTNAAPINLIQTRR499 pKa = 11.84SIGTTYY505 pKa = 10.18MFYY508 pKa = 10.57LYY510 pKa = 10.56KK511 pKa = 10.63QIFTDD516 pKa = 3.48NGNLSVEE523 pKa = 4.56AEE525 pKa = 4.28HH526 pKa = 6.99NMTTGVIVNLKK537 pKa = 8.58PTIHH541 pKa = 6.18SCKK544 pKa = 10.28SVNN547 pKa = 3.24

Molecular weight: 60.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

810

263

547

405.0

45.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.667 ± 0.322

1.358 ± 0.09

4.938 ± 0.213

3.58 ± 1.173

2.84 ± 0.322

6.914 ± 0.879

2.346 ± 0.175

6.667 ± 0.308

7.284 ± 0.453

5.802 ± 0.576

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.099 ± 0.319

5.432 ± 0.691

5.185 ± 0.286

1.605 ± 0.374

6.79 ± 0.03

9.136 ± 0.636

7.407 ± 1.572

6.914 ± 0.172

2.222 ± 0.453

4.815 ± 0.701
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski