Bacillus sp. V3-13

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus; unclassified Bacillus (in: Bacteria)

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4299 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2N5GCD2|A0A2N5GCD2_9BACI Aquaporin OS=Bacillus sp. V3-13 OX=2053728 GN=CU633_05330 PE=3 SV=1
MM1 pKa = 7.04EE2 pKa = 4.78QLYY5 pKa = 10.66QNAVEE10 pKa = 4.8ILNNMIPVPWEE21 pKa = 3.84KK22 pKa = 9.99IYY24 pKa = 10.85LYY26 pKa = 11.12AEE28 pKa = 4.0VSEE31 pKa = 5.16DD32 pKa = 2.89SRR34 pKa = 11.84QIYY37 pKa = 9.57FYY39 pKa = 9.93FYY41 pKa = 10.42PEE43 pKa = 4.68GEE45 pKa = 4.45TVPVYY50 pKa = 11.06SLDD53 pKa = 3.3IVKK56 pKa = 10.26KK57 pKa = 10.8FNLQEE62 pKa = 4.4DD63 pKa = 4.16DD64 pKa = 4.6FEE66 pKa = 4.34QLEE69 pKa = 4.5DD70 pKa = 3.65EE71 pKa = 5.54LYY73 pKa = 11.05DD74 pKa = 3.72CFTEE78 pKa = 3.58IWEE81 pKa = 4.2EE82 pKa = 3.84FGNQKK87 pKa = 8.08QEE89 pKa = 3.14KK90 pKa = 6.79WTYY93 pKa = 9.2LTFTLDD99 pKa = 3.1HH100 pKa = 6.73TGDD103 pKa = 3.58FDD105 pKa = 5.01IEE107 pKa = 4.26YY108 pKa = 10.1NYY110 pKa = 11.13NDD112 pKa = 3.64LSEE115 pKa = 4.21VDD117 pKa = 4.44SYY119 pKa = 10.91EE120 pKa = 3.77QQIIWEE126 pKa = 4.09YY127 pKa = 11.09RR128 pKa = 11.84NLGLITEE135 pKa = 4.68GEE137 pKa = 4.08RR138 pKa = 11.84PKK140 pKa = 10.98AIIEE144 pKa = 4.1KK145 pKa = 10.5YY146 pKa = 9.91IAEE149 pKa = 4.23KK150 pKa = 10.38QQ151 pKa = 3.31

Molecular weight: 18.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2N5GC92|A0A2N5GC92_9BACI SMI1/KNR4 family protein OS=Bacillus sp. V3-13 OX=2053728 GN=CU633_06055 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.24QPNKK9 pKa = 8.16RR10 pKa = 11.84KK11 pKa = 9.54HH12 pKa = 5.99SKK14 pKa = 8.79VHH16 pKa = 5.85GFRR19 pKa = 11.84SRR21 pKa = 11.84MSTANGRR28 pKa = 11.84KK29 pKa = 8.48VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.05GRR39 pKa = 11.84KK40 pKa = 8.7VLSAA44 pKa = 4.05

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4299

0

4299

1262598

22

2698

293.7

32.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.562 ± 0.041

0.745 ± 0.011

5.035 ± 0.029

7.493 ± 0.043

4.57 ± 0.032

7.149 ± 0.037

2.061 ± 0.017

7.832 ± 0.04

6.744 ± 0.033

9.848 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.634 ± 0.016

4.371 ± 0.028

3.766 ± 0.022

3.612 ± 0.027

4.229 ± 0.027

5.869 ± 0.026

5.173 ± 0.028

6.85 ± 0.026

1.019 ± 0.014

3.439 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski