Mycobacterium leprae (strain TN)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium leprae

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1603 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q9CCE3|PURNU_MYCLE Purine nucleoside phosphorylase ML0918 OS=Mycobacterium leprae (strain TN) OX=272631 GN=ML0918 PE=3 SV=1
MM1 pKa = 7.62LIIALVLALIGLVVLVFAVATSNLLMAWVCIGASVLGVLLLIVDD45 pKa = 4.6AVRR48 pKa = 11.84EE49 pKa = 4.19HH50 pKa = 5.88QCIDD54 pKa = 3.04AANNEE59 pKa = 4.44DD60 pKa = 6.06KK61 pKa = 11.12EE62 pKa = 4.88DD63 pKa = 3.71TDD65 pKa = 3.78QDD67 pKa = 3.73DD68 pKa = 3.74GAVYY72 pKa = 10.7VDD74 pKa = 4.54YY75 pKa = 11.01LDD77 pKa = 4.38EE78 pKa = 4.6VPAGTSTEE86 pKa = 4.03APDD89 pKa = 4.34AGSQEE94 pKa = 4.05GDD96 pKa = 3.68TNSGEE101 pKa = 4.09LSGYY105 pKa = 8.67WGRR108 pKa = 11.84LTIDD112 pKa = 3.21TGEE115 pKa = 4.12QSAVAADD122 pKa = 3.71DD123 pKa = 4.13HH124 pKa = 7.35DD125 pKa = 4.24NDD127 pKa = 4.4RR128 pKa = 11.84ATT130 pKa = 3.8

Molecular weight: 13.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P30768|EFTU_MYCLE Elongation factor Tu OS=Mycobacterium leprae (strain TN) OX=272631 GN=tuf PE=3 SV=2
MM1 pKa = 7.4GSVIKK6 pKa = 10.38KK7 pKa = 8.42RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.06RR11 pKa = 11.84MSKK14 pKa = 9.76KK15 pKa = 9.54KK16 pKa = 9.72HH17 pKa = 5.63RR18 pKa = 11.84KK19 pKa = 7.56LLRR22 pKa = 11.84RR23 pKa = 11.84TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84KK30 pKa = 10.11LGKK33 pKa = 9.87

Molecular weight: 4.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1603

0

1603

538623

33

3076

336.0

36.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.728 ± 0.075

0.953 ± 0.019

5.896 ± 0.046

5.083 ± 0.053

2.978 ± 0.034

8.43 ± 0.055

2.227 ± 0.027

4.84 ± 0.042

2.677 ± 0.047

10.027 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.107 ± 0.022

2.655 ± 0.03

5.376 ± 0.053

3.276 ± 0.029

6.956 ± 0.052

5.967 ± 0.043

6.102 ± 0.043

9.099 ± 0.058

1.383 ± 0.027

2.241 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski