Mycobacterium phage Validus

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Timquatrovirus; unclassified Timquatrovirus

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 105 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V5UPT0|V5UPT0_9CAUD Uncharacterized protein OS=Mycobacterium phage Validus OX=1414747 GN=11 PE=4 SV=1
MM1 pKa = 7.8RR2 pKa = 11.84APATVEE8 pKa = 4.48VPCPACGEE16 pKa = 4.67PITLALGFEE25 pKa = 4.64VVEE28 pKa = 4.5PEE30 pKa = 4.22PDD32 pKa = 3.06ATTAPVRR39 pKa = 11.84VHH41 pKa = 6.61PVDD44 pKa = 3.43LTEE47 pKa = 4.29RR48 pKa = 11.84AQEE51 pKa = 4.13HH52 pKa = 6.65GEE54 pKa = 4.13VCPVLTGGGSDD65 pKa = 3.55GG66 pKa = 4.05

Molecular weight: 6.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V5URR6|V5URR6_9CAUD Uncharacterized protein OS=Mycobacterium phage Validus OX=1414747 GN=92 PE=4 SV=1
MM1 pKa = 7.08TRR3 pKa = 11.84RR4 pKa = 11.84AAAQQMAFDD13 pKa = 4.34GLGEE17 pKa = 4.09QLTFSPAPAPIARR30 pKa = 11.84PAVRR34 pKa = 11.84PAVAAKK40 pKa = 8.27PAHH43 pKa = 6.09MSMAEE48 pKa = 3.89ARR50 pKa = 11.84RR51 pKa = 11.84IATDD55 pKa = 4.5LIAEE59 pKa = 4.62HH60 pKa = 7.0GLTGWTVTFDD70 pKa = 3.41NARR73 pKa = 11.84RR74 pKa = 11.84RR75 pKa = 11.84AGVCKK80 pKa = 10.56YY81 pKa = 7.29GTRR84 pKa = 11.84TIGLSKK90 pKa = 10.44PLMAQRR96 pKa = 11.84SWDD99 pKa = 4.05DD100 pKa = 2.52TWMTITHH107 pKa = 6.33EE108 pKa = 4.23VAHH111 pKa = 6.43ALVGHH116 pKa = 5.44SHH118 pKa = 5.66GHH120 pKa = 5.71DD121 pKa = 3.29AVWAAKK127 pKa = 9.58HH128 pKa = 5.99RR129 pKa = 11.84SLGGNGKK136 pKa = 9.49RR137 pKa = 11.84CFEE140 pKa = 4.78HH141 pKa = 8.69LDD143 pKa = 3.41EE144 pKa = 4.51TAPWMGTCSHH154 pKa = 6.25GKK156 pKa = 9.54KK157 pKa = 8.95FARR160 pKa = 11.84YY161 pKa = 7.72RR162 pKa = 11.84APKK165 pKa = 10.21RR166 pKa = 11.84LDD168 pKa = 2.87GWRR171 pKa = 11.84CKK173 pKa = 10.46CPAGSSPVVWEE184 pKa = 3.91HH185 pKa = 6.36RR186 pKa = 11.84RR187 pKa = 3.43

Molecular weight: 20.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

105

0

105

19674

37

1385

187.4

20.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.5 ± 0.47

1.154 ± 0.132

6.47 ± 0.259

5.855 ± 0.299

2.648 ± 0.147

8.763 ± 0.357

2.343 ± 0.16

3.624 ± 0.18

2.887 ± 0.175

8.265 ± 0.219

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.272 ± 0.089

2.521 ± 0.18

5.901 ± 0.246

3.217 ± 0.142

7.589 ± 0.345

4.956 ± 0.215

5.718 ± 0.172

7.919 ± 0.256

2.059 ± 0.1

2.338 ± 0.148
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski