Feline coronavirus

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae; Alphacoronavirus; Tegacovirus; Alphacoronavirus 1

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A125R5H5|A0A125R5H5_9ALPC Envelope small membrane protein OS=Feline coronavirus OX=12663 GN=E PE=3 SV=1
MM1 pKa = 7.71DD2 pKa = 3.9TVKK5 pKa = 10.85SIGISVDD12 pKa = 3.2AVLDD16 pKa = 3.96EE17 pKa = 4.93LDD19 pKa = 4.3SIAFAVTLKK28 pKa = 11.02VLFTSGKK35 pKa = 9.89LLVCIGFGDD44 pKa = 3.81TFEE47 pKa = 4.33EE48 pKa = 4.75AEE50 pKa = 4.06QKK52 pKa = 10.84AYY54 pKa = 10.67AKK56 pKa = 10.13LQLDD60 pKa = 3.62IEE62 pKa = 4.51EE63 pKa = 4.82SSNHH67 pKa = 4.24TTVV70 pKa = 2.72

Molecular weight: 7.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A125R5F0|A0A125R5F0_9ALPC Accessory protein 3a OS=Feline coronavirus OX=12663 PE=4 SV=1
MM1 pKa = 7.77PNFSWILKK9 pKa = 8.82SRR11 pKa = 11.84LIIRR15 pKa = 11.84LFNITVYY22 pKa = 10.61DD23 pKa = 4.52FCAKK27 pKa = 9.51NWYY30 pKa = 9.24KK31 pKa = 11.03LPFAVRR37 pKa = 11.84LRR39 pKa = 11.84IINNTKK45 pKa = 10.0PKK47 pKa = 8.73TASTIKK53 pKa = 9.77RR54 pKa = 11.84RR55 pKa = 11.84RR56 pKa = 11.84RR57 pKa = 11.84VVDD60 pKa = 3.12YY61 pKa = 11.08RR62 pKa = 11.84KK63 pKa = 9.65IAILNAARR71 pKa = 11.84KK72 pKa = 9.03

Molecular weight: 8.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11

0

11

13599

70

6696

1236.3

138.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.361 ± 0.181

3.03 ± 0.284

5.684 ± 0.336

4.53 ± 0.231

5.559 ± 0.196

6.221 ± 0.257

1.905 ± 0.246

5.53 ± 0.361

6.324 ± 0.619

8.655 ± 0.595

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.346 ± 0.256

5.861 ± 0.359

3.294 ± 0.137

3.008 ± 0.502

3.096 ± 0.531

6.662 ± 0.244

6.331 ± 0.319

9.677 ± 0.515

1.191 ± 0.206

4.736 ± 0.221
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski