Acidihalobacter ferrooxydans

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Chromatiales; Ectothiorhodospiraceae; Acidihalobacter

Average proteome isoelectric point is 6.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3065 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1P8UKD5|A0A1P8UKD5_9GAMM Type I-U CRISPR-associated protein Cas5/Cas6 OS=Acidihalobacter ferrooxydans OX=1765967 GN=BW247_15370 PE=4 SV=1
MM1 pKa = 7.53SATDD5 pKa = 4.09LLEE8 pKa = 4.0EE9 pKa = 4.25TMPDD13 pKa = 3.3PLLFTDD19 pKa = 4.27SAASKK24 pKa = 9.93VKK26 pKa = 10.36EE27 pKa = 4.34LIEE30 pKa = 4.08EE31 pKa = 4.27EE32 pKa = 4.25NNNEE36 pKa = 3.6LKK38 pKa = 10.94LRR40 pKa = 11.84VFVTGGGCSGFQYY53 pKa = 11.01GFTFDD58 pKa = 4.44EE59 pKa = 4.41NVGDD63 pKa = 3.89GDD65 pKa = 4.07TVVEE69 pKa = 4.21NGGVTLLIDD78 pKa = 3.8PMSYY82 pKa = 10.31QYY84 pKa = 11.58LVGAEE89 pKa = 3.64IDD91 pKa = 3.75YY92 pKa = 10.81TEE94 pKa = 4.44GLEE97 pKa = 4.19GAQFVIRR104 pKa = 11.84NPNATTTCGCGSSFSVV120 pKa = 3.54

Molecular weight: 12.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1P8UL06|A0A1P8UL06_9GAMM Riboflavin synthase OS=Acidihalobacter ferrooxydans OX=1765967 GN=BW247_02390 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLHH11 pKa = 6.3RR12 pKa = 11.84KK13 pKa = 6.84RR14 pKa = 11.84THH16 pKa = 5.75GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 10.19AGRR28 pKa = 11.84AVIASRR34 pKa = 11.84RR35 pKa = 11.84SKK37 pKa = 10.61GRR39 pKa = 11.84ARR41 pKa = 11.84LTPP44 pKa = 3.93

Molecular weight: 5.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3065

0

3065

965551

37

1930

315.0

34.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.103 ± 0.063

0.909 ± 0.013

5.347 ± 0.036

5.447 ± 0.04

3.465 ± 0.026

8.113 ± 0.041

2.635 ± 0.023

4.822 ± 0.033

2.847 ± 0.037

11.333 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.339 ± 0.022

2.666 ± 0.031

5.158 ± 0.035

3.741 ± 0.029

7.557 ± 0.05

5.014 ± 0.035

5.161 ± 0.03

7.238 ± 0.045

1.475 ± 0.02

2.631 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski