Sphingobacterium sp. (strain 21)

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Sphingobacteriia; Sphingobacteriales; Sphingobacteriaceae; Sphingobacterium; unclassified Sphingobacterium

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5132 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F4C2L5|F4C2L5_SPHS2 Uncharacterized protein OS=Sphingobacterium sp. (strain 21) OX=743722 GN=Sph21_3940 PE=4 SV=1
MM1 pKa = 7.19YY2 pKa = 7.77WTLEE6 pKa = 4.21LASHH10 pKa = 7.01LEE12 pKa = 4.08DD13 pKa = 4.93APWPATKK20 pKa = 10.54DD21 pKa = 3.44EE22 pKa = 5.26LIDD25 pKa = 3.48YY26 pKa = 8.56AIRR29 pKa = 11.84SGAPEE34 pKa = 3.96GVLEE38 pKa = 4.46NLNEE42 pKa = 4.04MEE44 pKa = 5.68DD45 pKa = 3.56DD46 pKa = 4.11GEE48 pKa = 4.79AYY50 pKa = 10.07EE51 pKa = 4.91SIEE54 pKa = 4.45EE55 pKa = 4.02VWPDD59 pKa = 3.82YY60 pKa = 7.77PTKK63 pKa = 10.9DD64 pKa = 2.91DD65 pKa = 4.08FLFNEE70 pKa = 4.77DD71 pKa = 3.58EE72 pKa = 4.45YY73 pKa = 12.0

Molecular weight: 8.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F4C2Y1|F4C2Y1_SPHS2 Dihydroorotase multifunctional complex type OS=Sphingobacterium sp. (strain 21) OX=743722 GN=Sph21_5136 PE=4 SV=1
MM1 pKa = 7.51TFTLVNLIRR10 pKa = 11.84PLSAFLLMTAFLLLSYY26 pKa = 10.58CPLRR30 pKa = 11.84RR31 pKa = 11.84TIQNLIKK38 pKa = 9.83GTQEE42 pKa = 3.93TEE44 pKa = 3.54RR45 pKa = 11.84SADD48 pKa = 3.18GRR50 pKa = 11.84QAGLAICQGIIDD62 pKa = 4.24SSGSTFNVPAPKK74 pKa = 10.23SAVNVPIFTAIVLSLFYY91 pKa = 10.46SIRR94 pKa = 11.84RR95 pKa = 11.84LLPSEE100 pKa = 4.3RR101 pKa = 11.84LVLQRR106 pKa = 11.84KK107 pKa = 8.99SLVAYY112 pKa = 8.51PNAVPIYY119 pKa = 9.34LRR121 pKa = 11.84NRR123 pKa = 11.84ILLII127 pKa = 3.95

Molecular weight: 14.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5132

0

5132

1809840

30

5215

352.7

39.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.343 ± 0.033

0.762 ± 0.011

5.288 ± 0.021

5.984 ± 0.029

4.896 ± 0.025

6.771 ± 0.036

1.99 ± 0.018

7.213 ± 0.033

6.643 ± 0.028

9.9 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.222 ± 0.014

5.407 ± 0.035

3.793 ± 0.019

3.902 ± 0.019

4.499 ± 0.024

6.33 ± 0.026

5.407 ± 0.029

6.156 ± 0.028

1.293 ± 0.015

4.2 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski