Synechococcus phage ACG-2014h

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 5.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 221 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V5UTF6|V5UTF6_9CAUD Uncharacterized protein OS=Synechococcus phage ACG-2014h OX=1340810 GN=S-MbCM7_221 PE=4 SV=1
MM1 pKa = 7.43IKK3 pKa = 10.6ALFSFVFAAVMWVQVPQWSDD23 pKa = 2.88DD24 pKa = 3.33WSKK27 pKa = 11.17CAVDD31 pKa = 4.84VPDD34 pKa = 4.93TACHH38 pKa = 6.28WYY40 pKa = 8.12ITAPDD45 pKa = 3.4STMGVGFSWEE55 pKa = 3.83NAPWFSAEE63 pKa = 3.73GLLDD67 pKa = 3.36IGEE70 pKa = 4.61LHH72 pKa = 7.1DD73 pKa = 4.52TMTSLQINSMVDD85 pKa = 3.02SS86 pKa = 4.28

Molecular weight: 9.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V5US08|V5US08_9CAUD Uncharacterized protein OS=Synechococcus phage ACG-2014h OX=1340810 GN=S-MbCM7_009 PE=4 SV=1
MM1 pKa = 7.81AYY3 pKa = 10.27SGFYY7 pKa = 10.61KK8 pKa = 10.33PVNPKK13 pKa = 9.93KK14 pKa = 10.79YY15 pKa = 8.94RR16 pKa = 11.84GNPTRR21 pKa = 11.84VIYY24 pKa = 10.45RR25 pKa = 11.84SLWEE29 pKa = 3.81RR30 pKa = 11.84KK31 pKa = 9.7FMVFCDD37 pKa = 3.62NNPAVIEE44 pKa = 4.2WGSEE48 pKa = 3.82EE49 pKa = 4.8VIIPYY54 pKa = 9.61RR55 pKa = 11.84APDD58 pKa = 3.54GKK60 pKa = 10.28VRR62 pKa = 11.84RR63 pKa = 11.84YY64 pKa = 10.18FPDD67 pKa = 4.53FYY69 pKa = 10.89IKK71 pKa = 10.56VRR73 pKa = 11.84EE74 pKa = 4.12KK75 pKa = 10.02TGKK78 pKa = 6.83LTKK81 pKa = 10.45YY82 pKa = 10.15IIEE85 pKa = 4.47IKK87 pKa = 10.07PKK89 pKa = 10.53RR90 pKa = 11.84QTQPPNDD97 pKa = 3.98KK98 pKa = 10.49NKK100 pKa = 8.78KK101 pKa = 5.83TAAYY105 pKa = 9.61RR106 pKa = 11.84NAALTFAKK114 pKa = 10.41NQSKK118 pKa = 9.38WSAARR123 pKa = 11.84EE124 pKa = 4.08YY125 pKa = 11.43CEE127 pKa = 3.96DD128 pKa = 3.36RR129 pKa = 11.84QMNFLILTEE138 pKa = 4.12DD139 pKa = 3.33HH140 pKa = 6.95LGVV143 pKa = 3.66

Molecular weight: 16.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

221

0

221

59917

32

3724

271.1

29.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.105 ± 0.187

0.808 ± 0.082

6.594 ± 0.117

5.903 ± 0.311

4.311 ± 0.107

7.918 ± 0.301

1.382 ± 0.133

6.334 ± 0.148

5.474 ± 0.378

7.2 ± 0.156

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.001 ± 0.223

6.189 ± 0.258

3.924 ± 0.129

3.56 ± 0.075

4.041 ± 0.13

7.272 ± 0.277

7.888 ± 0.431

6.758 ± 0.227

1.155 ± 0.097

4.186 ± 0.158
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski