Mycobacterium phage Malithi

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Pclasvirinae; Fishburnevirus; Mycobacterium virus Malithi

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 79 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B5H259|A0A0B5H259_9CAUD Uncharacterized protein OS=Mycobacterium phage Malithi OX=1567472 GN=41 PE=4 SV=1
MM1 pKa = 7.78SDD3 pKa = 3.48YY4 pKa = 11.22LDD6 pKa = 3.73ASGITDD12 pKa = 3.67AGLPDD17 pKa = 4.41EE18 pKa = 5.28YY19 pKa = 11.43LDD21 pKa = 5.68LDD23 pKa = 4.07EE24 pKa = 6.63LDD26 pKa = 4.74LPRR29 pKa = 11.84STAPQCEE36 pKa = 4.55HH37 pKa = 7.08PPVDD41 pKa = 4.4CTAEE45 pKa = 4.3CPGVRR50 pKa = 11.84ARR52 pKa = 11.84STVARR57 pKa = 11.84ILVTLGTTLVALALVLALTFGAVLAA82 pKa = 4.99

Molecular weight: 8.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B5HDI8|A0A0B5HDI8_9CAUD Uncharacterized protein OS=Mycobacterium phage Malithi OX=1567472 GN=69 PE=4 SV=1
MM1 pKa = 7.6KK2 pKa = 10.31LFKK5 pKa = 10.34RR6 pKa = 11.84QPTAIEE12 pKa = 3.81LHH14 pKa = 6.15HH15 pKa = 6.57RR16 pKa = 11.84AILEE20 pKa = 3.78WRR22 pKa = 11.84RR23 pKa = 11.84NPTISTYY30 pKa = 11.23VMVDD34 pKa = 2.71GRR36 pKa = 11.84EE37 pKa = 3.9VRR39 pKa = 11.84RR40 pKa = 11.84RR41 pKa = 11.84AVDD44 pKa = 3.21PLTRR48 pKa = 11.84LGSLLDD54 pKa = 3.64RR55 pKa = 11.84VMDD58 pKa = 5.12TIADD62 pKa = 3.74VCGIPAPRR70 pKa = 11.84LGTVPGAPCC79 pKa = 4.47

Molecular weight: 8.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

79

0

79

15318

29

1216

193.9

21.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.306 ± 0.491

1.025 ± 0.131

6.587 ± 0.179

6.006 ± 0.306

2.807 ± 0.169

8.787 ± 0.538

1.926 ± 0.18

4.276 ± 0.172

3.049 ± 0.182

7.925 ± 0.236

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.004 ± 0.107

2.964 ± 0.189

6.339 ± 0.208

3.734 ± 0.222

7.318 ± 0.438

5.405 ± 0.269

6.476 ± 0.253

6.985 ± 0.339

1.887 ± 0.155

2.193 ± 0.118
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski