Koyama Hill virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Sedoreovirinae; Orbivirus; Umatilla virus

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A077JHF2|A0A077JHF2_9REOV Core protein VP4 OS=Koyama Hill virus OX=1435294 GN=VP4 PE=3 SV=1
MM1 pKa = 7.88RR2 pKa = 11.84IVLLAPGDD10 pKa = 3.72IIKK13 pKa = 10.84NSEE16 pKa = 4.09PEE18 pKa = 4.0LKK20 pKa = 10.19QRR22 pKa = 11.84GIDD25 pKa = 3.51VSVKK29 pKa = 10.56DD30 pKa = 3.82WIEE33 pKa = 3.89NDD35 pKa = 3.4GSQLSEE41 pKa = 4.3GEE43 pKa = 4.23GKK45 pKa = 10.5GGRR48 pKa = 11.84EE49 pKa = 4.25GEE51 pKa = 4.15NGKK54 pKa = 8.91QASANSGRR62 pKa = 11.84RR63 pKa = 11.84DD64 pKa = 3.17GDD66 pKa = 3.42VGRR69 pKa = 11.84DD70 pKa = 3.53GAEE73 pKa = 3.7SSGDD77 pKa = 3.13GKK79 pKa = 11.15ANDD82 pKa = 4.37DD83 pKa = 4.29PRR85 pKa = 11.84PGDD88 pKa = 3.35SGRR91 pKa = 11.84IRR93 pKa = 11.84ADD95 pKa = 3.1GKK97 pKa = 9.57RR98 pKa = 11.84TVEE101 pKa = 4.3TEE103 pKa = 3.93SKK105 pKa = 9.37TGDD108 pKa = 3.11GGEE111 pKa = 3.99IRR113 pKa = 11.84DD114 pKa = 4.18GKK116 pKa = 10.29SASDD120 pKa = 3.59KK121 pKa = 10.58GSKK124 pKa = 8.06NAKK127 pKa = 9.28GNEE130 pKa = 3.7RR131 pKa = 11.84SIDD134 pKa = 3.56STVITSEE141 pKa = 3.99KK142 pKa = 10.0VKK144 pKa = 10.5EE145 pKa = 4.12KK146 pKa = 10.77EE147 pKa = 4.14GTDD150 pKa = 3.49SKK152 pKa = 10.85IAEE155 pKa = 4.06QLAEE159 pKa = 4.1KK160 pKa = 10.57RR161 pKa = 11.84GDD163 pKa = 3.61EE164 pKa = 3.96PSGSNGHH171 pKa = 6.48IYY173 pKa = 10.96VLTRR177 pKa = 11.84AVADD181 pKa = 5.07AINQRR186 pKa = 11.84SGTNLDD192 pKa = 3.47VLKK195 pKa = 10.69SLKK198 pKa = 10.61DD199 pKa = 3.29LGEE202 pKa = 4.19GKK204 pKa = 9.9KK205 pKa = 10.36CYY207 pKa = 9.95QISNGVGKK215 pKa = 10.8LIGVDD220 pKa = 3.02VDD222 pKa = 3.81AVKK225 pKa = 10.49EE226 pKa = 4.0QIDD229 pKa = 3.62ALNEE233 pKa = 3.63LKK235 pKa = 10.7AQYY238 pKa = 10.48DD239 pKa = 3.95GSNVNALLKK248 pKa = 10.0RR249 pKa = 11.84VVRR252 pKa = 11.84IEE254 pKa = 4.07SGGKK258 pKa = 9.33LDD260 pKa = 3.87QVFPKK265 pKa = 10.78GRR267 pKa = 11.84DD268 pKa = 3.47EE269 pKa = 3.95KK270 pKa = 11.07HH271 pKa = 5.65MPGSGVVLATNDD283 pKa = 3.58PKK285 pKa = 11.39YY286 pKa = 10.15IAQANAMFTCPTGDD300 pKa = 3.9TNWKK304 pKa = 8.68EE305 pKa = 4.3VSRR308 pKa = 11.84KK309 pKa = 8.28ATSKK313 pKa = 9.6TSIRR317 pKa = 11.84AYY319 pKa = 9.98SYY321 pKa = 11.53SEE323 pKa = 3.88IGDD326 pKa = 3.41GSEE329 pKa = 3.62IKK331 pKa = 10.43EE332 pKa = 4.27AFLSLIDD339 pKa = 3.84SLL341 pKa = 4.74

Molecular weight: 36.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A077JI40|A0A077JI40_9REOV T2 OS=Koyama Hill virus OX=1435294 GN=VP2 PE=3 SV=1
MM1 pKa = 7.51LEE3 pKa = 3.85AAEE6 pKa = 4.25RR7 pKa = 11.84AQEE10 pKa = 3.98RR11 pKa = 11.84SFTKK15 pKa = 10.56QEE17 pKa = 3.55IQQEE21 pKa = 4.13KK22 pKa = 10.46DD23 pKa = 3.34QIPLIEE29 pKa = 4.45GRR31 pKa = 11.84PNYY34 pKa = 7.62EE35 pKa = 3.82TGQSKK40 pKa = 10.61SSQLKK45 pKa = 8.41PLTRR49 pKa = 11.84FSFLNEE55 pKa = 3.48FNYY58 pKa = 10.18EE59 pKa = 3.98QEE61 pKa = 4.09NGFMYY66 pKa = 10.39QQLQDD71 pKa = 3.31KK72 pKa = 10.05AQALNILTNAVTSTTGANEE91 pKa = 3.81TLKK94 pKa = 11.01NEE96 pKa = 4.11KK97 pKa = 10.21AVFGATSDD105 pKa = 3.76ALKK108 pKa = 10.5DD109 pKa = 3.87DD110 pKa = 4.1PFTRR114 pKa = 11.84YY115 pKa = 7.66TKK117 pKa = 10.14QLAYY121 pKa = 7.81EE122 pKa = 4.2TTIQQINGQMAQKK135 pKa = 9.46RR136 pKa = 11.84RR137 pKa = 11.84KK138 pKa = 8.87MLILDD143 pKa = 4.85LIKK146 pKa = 10.37YY147 pKa = 6.24TLGVILLILLLWNMTVEE164 pKa = 4.69TIDD167 pKa = 3.47EE168 pKa = 4.22EE169 pKa = 4.86SYY171 pKa = 9.09KK172 pKa = 10.45TLLSGLFNRR181 pKa = 11.84NVTDD185 pKa = 3.4VSGMFSAARR194 pKa = 11.84IIRR197 pKa = 11.84RR198 pKa = 11.84YY199 pKa = 8.14TSYY202 pKa = 11.75LEE204 pKa = 4.13MASSIAMTLIIRR216 pKa = 11.84KK217 pKa = 8.82IVQLGMRR224 pKa = 11.84IRR226 pKa = 11.84TLEE229 pKa = 3.66RR230 pKa = 11.84DD231 pKa = 3.33RR232 pKa = 11.84VKK234 pKa = 10.87RR235 pKa = 11.84EE236 pKa = 3.86AYY238 pKa = 8.78NKK240 pKa = 8.0TVARR244 pKa = 11.84VTHH247 pKa = 6.62SGMCPPSAPALEE259 pKa = 4.42STEE262 pKa = 4.06RR263 pKa = 3.48

Molecular weight: 30.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10

0

10

6096

263

1299

609.6

69.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.283 ± 0.489

1.23 ± 0.209

5.676 ± 0.404

6.824 ± 0.434

3.74 ± 0.358

5.807 ± 0.504

2.657 ± 0.281

6.512 ± 0.281

5.299 ± 0.84

9.334 ± 0.51

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.887 ± 0.2

4.577 ± 0.294

3.593 ± 0.395

4.085 ± 0.366

7.037 ± 0.462

6.988 ± 0.589

6.217 ± 0.368

6.43 ± 0.333

1.05 ± 0.165

3.757 ± 0.349
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski