Candidatus Cyclonatronum proteinivorum

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Balneolaeota; unclassified Balneolaeota; Candidatus Cyclonatronum

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3251 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A345UJA3|A0A345UJA3_9BACT His Kinase A (Phospho-acceptor) domain-containing protein OS=Candidatus Cyclonatronum proteinivorum OX=1457365 GN=CYPRO_1298 PE=4 SV=1
MM1 pKa = 8.19LFMLLLWQVIPMSAVQANPPSCWGEE26 pKa = 4.25GNCDD30 pKa = 2.87MSRR33 pKa = 11.84PYY35 pKa = 10.34FQLNIDD41 pKa = 3.46KK42 pKa = 10.97SNYY45 pKa = 8.69VLPNGMEE52 pKa = 4.16YY53 pKa = 10.79NQVLNMQHH61 pKa = 6.82SNTKK65 pKa = 10.5SPDD68 pKa = 3.18LTFQSASTLGLTGATQIGQMAVDD91 pKa = 3.73AQGNRR96 pKa = 11.84YY97 pKa = 6.86VTGGFTGTISYY108 pKa = 10.65DD109 pKa = 3.43GVIVEE114 pKa = 4.49STGGYY119 pKa = 10.53DD120 pKa = 3.19VFIAKK125 pKa = 9.28FSPEE129 pKa = 4.07GTLLWYY135 pKa = 10.32QLAHH139 pKa = 6.22GSEE142 pKa = 4.31EE143 pKa = 4.17VEE145 pKa = 4.56EE146 pKa = 4.22EE147 pKa = 4.03FSLDD151 pKa = 3.23GGLTLAVDD159 pKa = 3.73DD160 pKa = 5.01HH161 pKa = 7.32GNVYY165 pKa = 10.72VGGAFVKK172 pKa = 10.16EE173 pKa = 3.74LHH175 pKa = 5.92FTDD178 pKa = 3.62NNGEE182 pKa = 4.17ILSSLSDD189 pKa = 3.52GRR191 pKa = 11.84DD192 pKa = 3.18DD193 pKa = 5.72DD194 pKa = 5.82LINLEE199 pKa = 4.59LFVAKK204 pKa = 10.53YY205 pKa = 7.72DD206 pKa = 3.94TNGNFQWALGGDD218 pKa = 4.02SGSEE222 pKa = 3.68ASPNSLEE229 pKa = 4.79LGINSVNSVMIDD241 pKa = 3.24TDD243 pKa = 4.16GYY245 pKa = 9.34PYY247 pKa = 10.76VAGGFSGTNLFGEE260 pKa = 4.65EE261 pKa = 3.91VTVQGEE267 pKa = 4.27SDD269 pKa = 3.7FFIASLDD276 pKa = 3.33KK277 pKa = 11.22DD278 pKa = 3.8GNYY281 pKa = 10.36LYY283 pKa = 9.74WANVFGTPGRR293 pKa = 11.84DD294 pKa = 3.08LASSISVDD302 pKa = 2.84ALGYY306 pKa = 10.79LNILGIVGEE315 pKa = 4.26GRR317 pKa = 11.84MYY319 pKa = 11.13LPDD322 pKa = 4.42SDD324 pKa = 5.41IYY326 pKa = 10.52WDD328 pKa = 3.96NDD330 pKa = 3.16TGNADD335 pKa = 3.86TFVISYY341 pKa = 9.76DD342 pKa = 4.05VNGEE346 pKa = 3.85WYY348 pKa = 9.51FASFMGAGDD357 pKa = 4.75DD358 pKa = 3.5IVGKK362 pKa = 9.89SVVSAADD369 pKa = 3.4GSFYY373 pKa = 11.17VGGFFSGTDD382 pKa = 3.25AYY384 pKa = 11.17FEE386 pKa = 5.08GYY388 pKa = 10.68NKK390 pKa = 9.66TFDD393 pKa = 4.06AMGTEE398 pKa = 4.37DD399 pKa = 4.85AFLVKK404 pKa = 10.42YY405 pKa = 10.59DD406 pKa = 3.89IDD408 pKa = 5.28GDD410 pKa = 4.57AIWVRR415 pKa = 11.84QFGFDD420 pKa = 3.24RR421 pKa = 11.84ATVDD425 pKa = 4.43VVTLDD430 pKa = 3.26EE431 pKa = 4.49NEE433 pKa = 4.09NVFVLGRR440 pKa = 11.84YY441 pKa = 8.95SDD443 pKa = 4.83SIIFDD448 pKa = 3.66IDD450 pKa = 3.29SDD452 pKa = 4.42SPVILTTEE460 pKa = 4.45SINNIYY466 pKa = 8.53MAKK469 pKa = 9.82FDD471 pKa = 3.85EE472 pKa = 4.74SGNFIWAKK480 pKa = 10.21NIEE483 pKa = 4.24GSGAEE488 pKa = 3.97SRR490 pKa = 11.84EE491 pKa = 3.91LVFDD495 pKa = 3.85RR496 pKa = 11.84EE497 pKa = 4.26TRR499 pKa = 11.84PFGTNPLDD507 pKa = 4.63IITSAYY513 pKa = 9.99NGGEE517 pKa = 4.03IILAGDD523 pKa = 3.97FDD525 pKa = 4.37GTLQLDD531 pKa = 4.96DD532 pKa = 3.89ITLTSDD538 pKa = 3.56GTRR541 pKa = 11.84KK542 pKa = 9.72IFMAVMPTGGAVSVGEE558 pKa = 4.7DD559 pKa = 3.42DD560 pKa = 5.24HH561 pKa = 7.01NIPTGIQLSQNYY573 pKa = 7.57PNPFNPTTNISFNLPQASQVSITVYY598 pKa = 9.28TVMGQRR604 pKa = 11.84VGTISNDD611 pKa = 2.66MFAAGQHH618 pKa = 4.57TVTWDD623 pKa = 3.2AAGLSSGTYY632 pKa = 8.75MYY634 pKa = 10.86RR635 pKa = 11.84LSADD639 pKa = 3.2NFSATRR645 pKa = 11.84KK646 pKa = 6.39MTLLKK651 pKa = 10.81

Molecular weight: 70.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A345UL86|A0A345UL86_9BACT Uncharacterized protein OS=Candidatus Cyclonatronum proteinivorum OX=1457365 GN=CYPRO_1988 PE=4 SV=1
MM1 pKa = 7.59LPLQGSRR8 pKa = 11.84VFRR11 pKa = 11.84IGHH14 pKa = 5.58PRR16 pKa = 11.84RR17 pKa = 11.84DD18 pKa = 3.37AKK20 pKa = 11.1SRR22 pKa = 11.84VSTVPGARR30 pKa = 11.84TNLRR34 pKa = 11.84PTTGCIPHH42 pKa = 7.52KK43 pKa = 10.14SRR45 pKa = 11.84KK46 pKa = 7.84IPKK49 pKa = 9.3IRR51 pKa = 11.84VLSSRR56 pKa = 11.84TLTLPCVASGAEE68 pKa = 4.2LPHH71 pKa = 6.9PGYY74 pKa = 10.59HH75 pKa = 6.92IPPQTNKK82 pKa = 9.11IRR84 pKa = 11.84HH85 pKa = 4.93PTYY88 pKa = 10.44AVTTINHH95 pKa = 5.09EE96 pKa = 4.36TVIPEE101 pKa = 4.09TLKK104 pKa = 10.34PKK106 pKa = 10.52QIITSKK112 pKa = 8.18HH113 pKa = 4.75TGVIRR118 pKa = 11.84DD119 pKa = 4.57LPTSACDD126 pKa = 4.24LPCSPPPKK134 pKa = 9.88RR135 pKa = 11.84CTRR138 pKa = 11.84LCRR141 pKa = 11.84TDD143 pKa = 2.95IAAGNSYY150 pKa = 10.76CGISRR155 pKa = 11.84LADD158 pKa = 3.73PGG160 pKa = 3.66

Molecular weight: 17.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3251

0

3251

1202591

29

4053

369.9

41.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.474 ± 0.042

0.684 ± 0.013

5.58 ± 0.03

6.714 ± 0.039

5.085 ± 0.036

7.398 ± 0.043

2.075 ± 0.024

6.095 ± 0.038

4.024 ± 0.062

9.942 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.418 ± 0.022

4.184 ± 0.039

4.616 ± 0.035

3.909 ± 0.031

5.93 ± 0.042

6.344 ± 0.03

5.713 ± 0.034

6.461 ± 0.032

1.247 ± 0.018

3.107 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski