Clostridium sp. 7_2_43FAA

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; unclassified Clostridium

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3389 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V9HIJ6|V9HIJ6_9CLOT DNA polymerase III subunit alpha OS=Clostridium sp. 7_2_43FAA OX=457396 GN=CSBG_03171 PE=3 SV=1
MM1 pKa = 7.31EE2 pKa = 4.54AHH4 pKa = 6.39VDD6 pKa = 3.11KK7 pKa = 10.05DD8 pKa = 3.68TCIGCGLCTSICEE21 pKa = 4.99SIFEE25 pKa = 4.29MDD27 pKa = 4.31DD28 pKa = 3.36DD29 pKa = 4.58GKK31 pKa = 11.05AVAKK35 pKa = 10.84NPDD38 pKa = 3.53VPSEE42 pKa = 4.42LEE44 pKa = 4.62DD45 pKa = 3.47EE46 pKa = 4.97AKK48 pKa = 10.53DD49 pKa = 3.91AEE51 pKa = 4.48ASCPVNAISVSS62 pKa = 3.42

Molecular weight: 6.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U6Q3B2|U6Q3B2_9CLOT Uncharacterized protein (Fragment) OS=Clostridium sp. 7_2_43FAA OX=457396 GN=CSBG_03507 PE=4 SV=1
MM1 pKa = 7.35GVMIPMKK8 pKa = 9.99RR9 pKa = 11.84EE10 pKa = 3.32RR11 pKa = 11.84MLTIRR16 pKa = 11.84VTDD19 pKa = 4.01DD20 pKa = 2.63EE21 pKa = 4.45HH22 pKa = 8.19ARR24 pKa = 11.84LLEE27 pKa = 4.22RR28 pKa = 11.84CEE30 pKa = 4.24GKK32 pKa = 9.89QLAVWMRR39 pKa = 11.84RR40 pKa = 11.84DD41 pKa = 3.37QRR43 pKa = 11.84KK44 pKa = 7.7ITQGQCQRR52 pKa = 11.84FVNTDD57 pKa = 2.83VGVPQGSQQHH67 pKa = 6.53PAMQIRR73 pKa = 11.84NIMVQGADD81 pKa = 3.44FRR83 pKa = 11.84VSRR86 pKa = 11.84LYY88 pKa = 8.57EE89 pKa = 3.7TRR91 pKa = 11.84KK92 pKa = 9.55PKK94 pKa = 9.73TIHH97 pKa = 5.97VVAQVADD104 pKa = 3.76VLQQQSLHH112 pKa = 4.94VRR114 pKa = 11.84SRR116 pKa = 11.84IGDD119 pKa = 3.82SFCC122 pKa = 5.7

Molecular weight: 14.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3389

0

3389

1046289

29

2856

308.7

34.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.428 ± 0.042

1.049 ± 0.017

5.644 ± 0.03

7.702 ± 0.049

4.388 ± 0.033

6.399 ± 0.036

1.216 ± 0.016

10.38 ± 0.049

8.981 ± 0.044

9.128 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.499 ± 0.019

6.779 ± 0.042

2.667 ± 0.023

2.048 ± 0.018

3.265 ± 0.027

6.43 ± 0.03

4.713 ± 0.031

6.41 ± 0.034

0.674 ± 0.012

4.199 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski