secondary endosymbiont of Trabutina mannipara

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Enterobacteriaceae incertae sedis; ant, tsetse, mealybug, aphid, etc. endosymbionts; mealybug secondary endosymbionts

Average proteome isoelectric point is 7.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 237 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1C3L415|A0A1C3L415_9ENTR tRNA (guanine-N(1)-)-methyltransferase OS=secondary endosymbiont of Trabutina mannipara OX=1835721 GN=trmD PE=3 SV=1
MM1 pKa = 7.08STIEE5 pKa = 4.6DD6 pKa = 3.81SVKK9 pKa = 10.6EE10 pKa = 4.05IIVDD14 pKa = 3.33QLGVKK19 pKa = 9.95KK20 pKa = 10.87EE21 pKa = 3.97EE22 pKa = 4.47VINSASFVDD31 pKa = 5.22DD32 pKa = 5.07LGADD36 pKa = 3.42SLDD39 pKa = 3.63TVEE42 pKa = 6.37LIMALEE48 pKa = 4.16EE49 pKa = 4.1EE50 pKa = 4.47FDD52 pKa = 4.19IEE54 pKa = 4.28ITDD57 pKa = 3.73EE58 pKa = 4.06EE59 pKa = 4.61AEE61 pKa = 4.76KK62 pKa = 9.69ITTVQAAIDD71 pKa = 4.17FIQYY75 pKa = 8.84NQQ77 pKa = 3.07

Molecular weight: 8.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1C3L489|A0A1C3L489_9ENTR Pseudouridine synthase OS=secondary endosymbiont of Trabutina mannipara OX=1835721 GN=rluC PE=3 SV=1
MM1 pKa = 7.36HH2 pKa = 7.05HH3 pKa = 6.91RR4 pKa = 11.84KK5 pKa = 9.3IGRR8 pKa = 11.84QLNRR12 pKa = 11.84NSSHH16 pKa = 6.34LKK18 pKa = 10.25AMFRR22 pKa = 11.84NMASSLVYY30 pKa = 10.12YY31 pKa = 9.04EE32 pKa = 4.67IIKK35 pKa = 7.68TTLPKK40 pKa = 10.57AKK42 pKa = 9.4EE43 pKa = 3.63LRR45 pKa = 11.84RR46 pKa = 11.84FVEE49 pKa = 4.04PLVTIAKK56 pKa = 9.39NDD58 pKa = 3.33SVANRR63 pKa = 11.84RR64 pKa = 11.84LAFSRR69 pKa = 11.84IRR71 pKa = 11.84DD72 pKa = 3.71NVIVTKK78 pKa = 10.5LFNEE82 pKa = 4.4LGPRR86 pKa = 11.84FKK88 pKa = 10.43IRR90 pKa = 11.84AGGYY94 pKa = 7.33TRR96 pKa = 11.84ILKK99 pKa = 10.54CGFRR103 pKa = 11.84TGDD106 pKa = 3.97NAPMAYY112 pKa = 9.04IEE114 pKa = 5.07FVDD117 pKa = 3.82RR118 pKa = 11.84DD119 pKa = 3.74KK120 pKa = 11.46KK121 pKa = 9.5ITEE124 pKa = 3.94

Molecular weight: 14.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

237

0

237

77885

38

1400

328.6

37.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.425 ± 0.131

1.208 ± 0.051

4.893 ± 0.094

5.827 ± 0.151

3.965 ± 0.1

6.189 ± 0.129

2.228 ± 0.067

9.933 ± 0.144

7.623 ± 0.129

9.817 ± 0.191

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.406 ± 0.069

6.011 ± 0.11

3.282 ± 0.07

3.59 ± 0.083

5.493 ± 0.093

5.81 ± 0.076

5.009 ± 0.08

6.002 ± 0.124

0.867 ± 0.055

3.424 ± 0.103
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski