Vibrio phage eugene 12A10

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 250 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R9UBE9|R9UBE9_9CAUD Uncharacterized protein OS=Vibrio phage eugene 12A10 OX=573172 GN=VPLG_00107 PE=4 SV=1
MM1 pKa = 8.07VEE3 pKa = 5.64FYY5 pKa = 10.16FQQMTNLIEE14 pKa = 4.36GLEE17 pKa = 4.22PEE19 pKa = 4.67DD20 pKa = 5.61FGNIWDD26 pKa = 4.32EE27 pKa = 3.93FWTLSRR33 pKa = 11.84LVDD36 pKa = 4.1NIKK39 pKa = 10.7HH40 pKa = 6.04IDD42 pKa = 3.9YY43 pKa = 10.39YY44 pKa = 11.53DD45 pKa = 4.86PDD47 pKa = 3.55TTYY50 pKa = 10.94FEE52 pKa = 6.49DD53 pKa = 3.18IMARR57 pKa = 11.84YY58 pKa = 9.87DD59 pKa = 3.77SINEE63 pKa = 3.98YY64 pKa = 10.62LEE66 pKa = 4.18KK67 pKa = 10.89

Molecular weight: 8.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R9UB87|R9UB87_9CAUD Uncharacterized protein OS=Vibrio phage eugene 12A10 OX=573172 GN=VPLG_00047 PE=4 SV=1
MM1 pKa = 6.89SQRR4 pKa = 11.84TIKK7 pKa = 10.59ASQKK11 pKa = 9.64RR12 pKa = 11.84AQQRR16 pKa = 11.84IIRR19 pKa = 11.84NKK21 pKa = 9.53SMAAMRR27 pKa = 11.84VKK29 pKa = 10.45SQPQDD34 pKa = 3.15RR35 pKa = 11.84AEE37 pKa = 3.96VLSTIKK43 pKa = 10.37RR44 pKa = 11.84INVVRR49 pKa = 11.84GG50 pKa = 3.23

Molecular weight: 5.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

250

0

250

40408

49

1080

161.6

18.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.041 ± 0.241

1.844 ± 0.111

6.434 ± 0.133

8.196 ± 0.2

4.338 ± 0.125

6.33 ± 0.172

2.151 ± 0.092

5.732 ± 0.133

8.043 ± 0.218

8.471 ± 0.154

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.534 ± 0.109

4.94 ± 0.135

3.007 ± 0.091

3.361 ± 0.112

4.239 ± 0.113

6.528 ± 0.167

6.088 ± 0.183

6.697 ± 0.147

1.668 ± 0.08

4.358 ± 0.133
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski