Thermocrinis minervae

Taxonomy: cellular organisms; Bacteria; Aquificae; Aquificae; Aquificales; Aquificaceae; Thermocrinis

Average proteome isoelectric point is 6.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1463 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1M6SCA8|A0A1M6SCA8_9AQUI 2-isopropylmalate synthase OS=Thermocrinis minervae OX=381751 GN=leuA PE=3 SV=1
MM1 pKa = 8.28DD2 pKa = 4.53EE3 pKa = 3.99RR4 pKa = 11.84LKK6 pKa = 11.01LIRR9 pKa = 11.84EE10 pKa = 4.28KK11 pKa = 11.13VLEE14 pKa = 4.19AVNNPDD20 pKa = 4.56LDD22 pKa = 4.07VEE24 pKa = 4.83VCFPEE29 pKa = 6.6ADD31 pKa = 3.92DD32 pKa = 4.24ACEE35 pKa = 3.94LKK37 pKa = 10.35PYY39 pKa = 10.13PYY41 pKa = 10.71LKK43 pKa = 10.1VKK45 pKa = 10.51YY46 pKa = 9.33VVEE49 pKa = 4.04GHH51 pKa = 6.75DD52 pKa = 3.7VYY54 pKa = 11.31EE55 pKa = 4.57KK56 pKa = 10.79EE57 pKa = 4.04IDD59 pKa = 3.53INPDD63 pKa = 2.98YY64 pKa = 11.01LLNSDD69 pKa = 3.48VQEE72 pKa = 4.11VVNYY76 pKa = 10.2ILFQVQQFMEE86 pKa = 4.9EE87 pKa = 4.04IDD89 pKa = 3.68SVEE92 pKa = 4.16YY93 pKa = 10.82GGEE96 pKa = 3.8

Molecular weight: 11.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1M6RCS7|A0A1M6RCS7_9AQUI TldD protein OS=Thermocrinis minervae OX=381751 GN=SAMN05444391_0591 PE=3 SV=1
MM1 pKa = 7.65ASARR5 pKa = 11.84NITHH9 pKa = 6.96ISNLKK14 pKa = 9.75RR15 pKa = 11.84KK16 pKa = 9.29RR17 pKa = 11.84KK18 pKa = 9.7SGFLARR24 pKa = 11.84MSTKK28 pKa = 10.23SGRR31 pKa = 11.84KK32 pKa = 7.51IIKK35 pKa = 9.47RR36 pKa = 11.84RR37 pKa = 11.84RR38 pKa = 11.84KK39 pKa = 9.47KK40 pKa = 10.11GRR42 pKa = 11.84HH43 pKa = 5.27RR44 pKa = 11.84LTPP47 pKa = 3.91

Molecular weight: 5.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1463

0

1463

437030

30

1565

298.7

33.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.084 ± 0.058

0.916 ± 0.024

4.888 ± 0.042

8.153 ± 0.071

4.674 ± 0.055

6.807 ± 0.058

1.72 ± 0.025

6.825 ± 0.051

7.76 ± 0.072

11.403 ± 0.08

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.234 ± 0.028

3.174 ± 0.038

4.085 ± 0.038

2.752 ± 0.035

5.336 ± 0.038

5.628 ± 0.054

4.169 ± 0.037

8.18 ± 0.056

0.927 ± 0.018

4.284 ± 0.048
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski