Limnothrix rosea IAM M-220

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Synechococcales; Pseudanabaenaceae; Limnothrix; Limnothrix rosea

Average proteome isoelectric point is 6.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3542 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Q4R4P3|A0A1Q4R4P3_9CYAN CheR-type methyltransferase domain-containing protein OS=Limnothrix rosea IAM M-220 OX=454133 GN=NIES208_09535 PE=4 SV=1
MM1 pKa = 7.86AIAPAAIQSEE11 pKa = 4.58RR12 pKa = 11.84NSVSANPFTEE22 pKa = 5.05DD23 pKa = 4.08SEE25 pKa = 5.26LPLDD29 pKa = 4.34LSLGEE34 pKa = 5.28LDD36 pKa = 3.93GSWRR40 pKa = 11.84QFRR43 pKa = 11.84IAGQYY48 pKa = 9.66EE49 pKa = 4.37FADD52 pKa = 4.77LMQTWFSLFGITNVYY67 pKa = 9.8GNTYY71 pKa = 7.98FTQGEE76 pKa = 4.39TTEE79 pKa = 4.32MGGKK83 pKa = 9.46EE84 pKa = 3.84YY85 pKa = 10.72LVAYY89 pKa = 7.93RR90 pKa = 11.84FPLAPQEE97 pKa = 4.5LDD99 pKa = 3.66LEE101 pKa = 4.65DD102 pKa = 3.7MFAVFDD108 pKa = 4.84SGQQCDD114 pKa = 3.44DD115 pKa = 3.37MSRR118 pKa = 11.84LEE120 pKa = 4.07AAILNEE126 pKa = 3.9NTKK129 pKa = 10.48VSLALLNPNTIGSLNDD145 pKa = 3.17IQTLDD150 pKa = 3.66VPGMLQASQEE160 pKa = 4.19QFAMMQEE167 pKa = 4.04TCAEE171 pKa = 4.18VNAEE175 pKa = 4.05NQVIEE180 pKa = 4.48ALDD183 pKa = 3.42NLGAINRR190 pKa = 11.84AQQDD194 pKa = 3.17FWLEE198 pKa = 3.97NKK200 pKa = 10.19RR201 pKa = 11.84FTDD204 pKa = 4.14NIEE207 pKa = 3.93SLQIGLSPEE216 pKa = 3.73TDD218 pKa = 2.94AYY220 pKa = 10.91SYY222 pKa = 11.44TMTLQNPSLVTSQAIAKK239 pKa = 9.82SPEE242 pKa = 4.12GYY244 pKa = 10.06HH245 pKa = 6.23IVGATHH251 pKa = 6.95VYY253 pKa = 11.23DD254 pKa = 5.28DD255 pKa = 4.77FDD257 pKa = 4.94SEE259 pKa = 4.73FPSTITILCEE269 pKa = 3.55KK270 pKa = 9.61SEE272 pKa = 4.38PGGPVPSLPFFDD284 pKa = 4.57EE285 pKa = 3.94ATNYY289 pKa = 8.41LACPLGTFEE298 pKa = 4.9TYY300 pKa = 10.66

Molecular weight: 33.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Q4R8P1|A0A1Q4R8P1_9CYAN Peptidase M16 OS=Limnothrix rosea IAM M-220 OX=454133 GN=NIES208_01680 PE=3 SV=1
MM1 pKa = 7.55TKK3 pKa = 8.94RR4 pKa = 11.84TLGGTVRR11 pKa = 11.84KK12 pKa = 9.36QKK14 pKa = 9.22RR15 pKa = 11.84TSGFRR20 pKa = 11.84ARR22 pKa = 11.84MRR24 pKa = 11.84SHH26 pKa = 6.52TGKK29 pKa = 10.03NVIRR33 pKa = 11.84ARR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.01KK38 pKa = 9.6GRR40 pKa = 11.84HH41 pKa = 5.0RR42 pKa = 11.84LSVV45 pKa = 3.12

Molecular weight: 5.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3542

0

3542

1119968

29

4116

316.2

35.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.482 ± 0.046

0.985 ± 0.015

5.509 ± 0.052

6.579 ± 0.045

4.194 ± 0.027

6.845 ± 0.093

1.872 ± 0.024

6.608 ± 0.032

4.771 ± 0.04

10.824 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.027 ± 0.019

4.026 ± 0.032

4.604 ± 0.037

5.132 ± 0.045

5.029 ± 0.038

6.037 ± 0.034

5.695 ± 0.033

6.44 ± 0.036

1.419 ± 0.02

2.921 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski