Bacillus sp. HMF5848

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus; unclassified Bacillus (in: Bacteria)

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4048 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3R9UEU3|A0A3R9UEU3_9BACI Branched-chain amino acid transport system carrier protein OS=Bacillus sp. HMF5848 OX=2495421 GN=brnQ PE=3 SV=1
MM1 pKa = 7.38AQNKK5 pKa = 8.94RR6 pKa = 11.84QIGINQEE13 pKa = 3.88EE14 pKa = 4.63TGCTRR19 pKa = 11.84TFSRR23 pKa = 11.84NITEE27 pKa = 3.96FAEE30 pKa = 5.51LIIEE34 pKa = 4.14PNIIAGTPTVEE45 pKa = 4.83CIGEE49 pKa = 4.0PEE51 pKa = 5.0IIDD54 pKa = 3.92MLPEE58 pKa = 4.44FDD60 pKa = 5.72DD61 pKa = 3.85NCTFNVSQDD70 pKa = 3.24LCVEE74 pKa = 4.36FDD76 pKa = 3.62VSFGANASVGANGLVCDD93 pKa = 4.71DD94 pKa = 5.41PIIGGCGTPPMGCVLTRR111 pKa = 11.84GRR113 pKa = 11.84YY114 pKa = 5.84QTDD117 pKa = 3.37EE118 pKa = 4.3EE119 pKa = 4.53FTNSIIEE126 pKa = 4.15AEE128 pKa = 4.53GGSIPLGNGGNFSILATTGNVADD151 pKa = 4.72IFDD154 pKa = 4.79GNPPAPAPDD163 pKa = 3.17IPQYY167 pKa = 8.17EE168 pKa = 4.35TLYY171 pKa = 10.55IQLLTAKK178 pKa = 10.32LNVARR183 pKa = 11.84GATCDD188 pKa = 3.45FATEE192 pKa = 4.96AIAAADD198 pKa = 3.57AFLSNVTPADD208 pKa = 3.54PTQASALADD217 pKa = 4.09DD218 pKa = 5.99LDD220 pKa = 4.31DD221 pKa = 4.8FNNGRR226 pKa = 11.84INEE229 pKa = 4.34CPQKK233 pKa = 10.83CDD235 pKa = 2.97

Molecular weight: 24.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A428J3U1|A0A428J3U1_9BACI OsmC family peroxiredoxin OS=Bacillus sp. HMF5848 OX=2495421 GN=EJF36_06465 PE=3 SV=1
MM1 pKa = 7.52FIAPNGVFIAPNRR14 pKa = 11.84VLIAPNRR21 pKa = 11.84VLIAPNRR28 pKa = 11.84VFIAPNRR35 pKa = 11.84VLIAPNRR42 pKa = 11.84VLIAPNRR49 pKa = 11.84VLIAPNGVSIAPNGVSIAPKK69 pKa = 10.52GVFTAPNLVLIAPKK83 pKa = 10.28GVSIAPKK90 pKa = 10.11GVLIAPNRR98 pKa = 11.84VLIAPNQVFIAPNRR112 pKa = 11.84VLIAPNGVLIAPNGVLIAPNGVSIAPNGVFITT144 pKa = 4.14

Molecular weight: 14.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4048

0

4048

1226140

26

2695

302.9

34.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.95 ± 0.048

0.739 ± 0.013

5.282 ± 0.032

7.097 ± 0.04

4.582 ± 0.031

6.424 ± 0.034

2.157 ± 0.021

8.119 ± 0.04

6.574 ± 0.036

9.718 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.71 ± 0.018

4.623 ± 0.027

3.503 ± 0.023

3.873 ± 0.026

3.925 ± 0.029

6.004 ± 0.026

5.711 ± 0.031

7.286 ± 0.031

1.009 ± 0.016

3.713 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski