Mycoplasma arthritidis (strain 158L3-1)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Mycoplasmatales; Mycoplasmataceae; Mycoplasma; Mycoplasma arthritidis

Average proteome isoelectric point is 7.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 631 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|B3PML9|RL32_MYCA5 50S ribosomal protein L32 OS=Mycoplasma arthritidis (strain 158L3-1) OX=243272 GN=rpmF PE=3 SV=1
MM1 pKa = 7.78NEE3 pKa = 4.45FKK5 pKa = 10.73RR6 pKa = 11.84LNQASFNNFIIAADD20 pKa = 4.58AIFSTWTIININDD33 pKa = 3.93KK34 pKa = 11.29EE35 pKa = 4.59DD36 pKa = 3.56MDD38 pKa = 5.64KK39 pKa = 10.87MFIDD43 pKa = 3.81NCEE46 pKa = 3.6MSNNYY51 pKa = 8.63LHH53 pKa = 7.53PYY55 pKa = 8.53YY56 pKa = 10.28QGEE59 pKa = 4.57VNNEE63 pKa = 3.9TLISLL68 pKa = 4.44

Molecular weight: 8.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|B3PMN7|RL14_MYCA5 50S ribosomal protein L14 OS=Mycoplasma arthritidis (strain 158L3-1) OX=243272 GN=rplN PE=3 SV=1
MM1 pKa = 7.35ARR3 pKa = 11.84KK4 pKa = 9.72SLMVKK9 pKa = 10.4AEE11 pKa = 4.04RR12 pKa = 11.84QPKK15 pKa = 9.49FKK17 pKa = 10.32VRR19 pKa = 11.84KK20 pKa = 6.25YY21 pKa = 8.67TRR23 pKa = 11.84CQICGRR29 pKa = 11.84VHH31 pKa = 6.76AVLRR35 pKa = 11.84KK36 pKa = 9.58YY37 pKa = 10.23KK38 pKa = 9.85ICRR41 pKa = 11.84ICFRR45 pKa = 11.84EE46 pKa = 3.88LAHH49 pKa = 6.54EE50 pKa = 4.41GKK52 pKa = 10.21IPGVKK57 pKa = 9.38KK58 pKa = 10.89ASWW61 pKa = 3.03

Molecular weight: 7.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

631

0

631

241600

37

2993

382.9

43.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.41 ± 0.134

0.5 ± 0.025

5.685 ± 0.105

7.449 ± 0.152

4.885 ± 0.135

4.321 ± 0.107

1.424 ± 0.035

8.874 ± 0.109

11.155 ± 0.213

9.888 ± 0.096

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.664 ± 0.053

7.191 ± 0.113

2.654 ± 0.076

3.444 ± 0.078

2.994 ± 0.07

6.594 ± 0.084

5.177 ± 0.057

5.031 ± 0.101

0.855 ± 0.03

3.803 ± 0.1
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski