Streptomyces sp. Ag109_O5-10

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8132 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H5PLM8|A0A1H5PLM8_9ACTN K+-transporting ATPase KdpF subunit OS=Streptomyces sp. Ag109_O5-10 OX=1855349 GN=SAMN05216533_7183 PE=4 SV=1
MM1 pKa = 6.64QQEE4 pKa = 3.98ALAGGEE10 pKa = 4.03ALEE13 pKa = 4.15VWIDD17 pKa = 3.41QDD19 pKa = 3.99LCTGDD24 pKa = 5.01GICAQYY30 pKa = 10.9APEE33 pKa = 4.23VFEE36 pKa = 5.9LDD38 pKa = 3.05IDD40 pKa = 3.65GLAYY44 pKa = 10.73VKK46 pKa = 10.18GTDD49 pKa = 5.28DD50 pKa = 4.79EE51 pKa = 4.82LLQAKK56 pKa = 9.02GATVGVPLPLLTDD69 pKa = 3.69VVDD72 pKa = 4.29SARR75 pKa = 11.84EE76 pKa = 3.94CPGEE80 pKa = 4.38CIHH83 pKa = 6.0VRR85 pKa = 11.84RR86 pKa = 11.84VADD89 pKa = 3.37QVEE92 pKa = 5.11VYY94 pKa = 10.88GPDD97 pKa = 3.35AEE99 pKa = 4.31

Molecular weight: 10.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H5MUJ4|A0A1H5MUJ4_9ACTN Ribosomal-protein-alanine N-acetyltransferase OS=Streptomyces sp. Ag109_O5-10 OX=1855349 GN=SAMN05216533_4376 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILANRR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.46GRR40 pKa = 11.84ASLSAA45 pKa = 3.83

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8132

0

8132

2675155

29

4601

329.0

35.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.598 ± 0.04

0.784 ± 0.008

5.953 ± 0.021

5.463 ± 0.027

2.743 ± 0.014

9.499 ± 0.026

2.344 ± 0.014

3.062 ± 0.018

2.138 ± 0.025

10.246 ± 0.028

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.681 ± 0.01

1.824 ± 0.017

6.069 ± 0.023

2.804 ± 0.014

8.012 ± 0.038

5.122 ± 0.024

6.356 ± 0.031

8.582 ± 0.024

1.546 ± 0.013

2.174 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski