Hubei sobemo-like virus 30

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KEK2|A0A1L3KEK2_9VIRU RNA-directed RNA polymerase OS=Hubei sobemo-like virus 30 OX=1923217 PE=4 SV=1
MM1 pKa = 7.59KK2 pKa = 10.25NLLSAFEE9 pKa = 4.16SVKK12 pKa = 10.1WSIRR16 pKa = 11.84KK17 pKa = 9.44DD18 pKa = 3.58FLSFDD23 pKa = 3.63HH24 pKa = 6.96FSDD27 pKa = 3.7VVRR30 pKa = 11.84NKK32 pKa = 10.12IEE34 pKa = 4.03WTSSPGYY41 pKa = 9.6PYY43 pKa = 11.29LLRR46 pKa = 11.84ATSNGIYY53 pKa = 10.37FNVKK57 pKa = 10.29NGIPDD62 pKa = 3.49EE63 pKa = 4.17NRR65 pKa = 11.84LLEE68 pKa = 4.29VYY70 pKa = 9.82QVVMSQINDD79 pKa = 3.42RR80 pKa = 11.84SSDD83 pKa = 4.0PIRR86 pKa = 11.84LFIKK90 pKa = 10.24QEE92 pKa = 3.93PLKK95 pKa = 10.82HH96 pKa = 6.03EE97 pKa = 4.45KK98 pKa = 10.31LDD100 pKa = 3.45SGRR103 pKa = 11.84YY104 pKa = 8.73RR105 pKa = 11.84LISSVSIIDD114 pKa = 3.68QIIDD118 pKa = 3.27HH119 pKa = 6.32MLFDD123 pKa = 5.63DD124 pKa = 4.65MNDD127 pKa = 3.73MITDD131 pKa = 3.2DD132 pKa = 3.78WMFVPPKK139 pKa = 9.8IGWSAFTGGWKK150 pKa = 10.44FMPKK154 pKa = 10.32LSEE157 pKa = 4.17QPWALDD163 pKa = 3.22KK164 pKa = 11.54SSWDD168 pKa = 3.06WTVNLWLFDD177 pKa = 3.27IVLDD181 pKa = 5.22MRR183 pKa = 11.84IQLCKK188 pKa = 9.96NLSRR192 pKa = 11.84EE193 pKa = 4.21WVDD196 pKa = 3.36LALWRR201 pKa = 11.84YY202 pKa = 7.49SQLYY206 pKa = 9.27IEE208 pKa = 5.55PIFVTTGGLLLKK220 pKa = 10.49QITPGVMKK228 pKa = 10.11SGCVNTIVDD237 pKa = 3.84NSIMQVILHH246 pKa = 6.11LRR248 pKa = 11.84VCFEE252 pKa = 3.83EE253 pKa = 5.66GIRR256 pKa = 11.84PDD258 pKa = 3.14WLFSLGDD265 pKa = 3.49DD266 pKa = 3.9TLQKK270 pKa = 10.68KK271 pKa = 8.5PAEE274 pKa = 4.03PEE276 pKa = 3.72RR277 pKa = 11.84YY278 pKa = 8.76LRR280 pKa = 11.84AMSKK284 pKa = 10.19YY285 pKa = 10.26AIVKK289 pKa = 8.59QAHH292 pKa = 6.82DD293 pKa = 3.59VTEE296 pKa = 4.47FAGHH300 pKa = 6.63RR301 pKa = 11.84FDD303 pKa = 5.59GIHH306 pKa = 5.82VEE308 pKa = 3.64PVYY311 pKa = 10.36RR312 pKa = 11.84SKK314 pKa = 10.96HH315 pKa = 5.35SYY317 pKa = 10.05ILLHH321 pKa = 6.3MDD323 pKa = 4.16PANLEE328 pKa = 3.99AMANSYY334 pKa = 10.69CLLYY338 pKa = 10.36HH339 pKa = 6.49RR340 pKa = 11.84SVYY343 pKa = 10.47RR344 pKa = 11.84DD345 pKa = 3.31VIRR348 pKa = 11.84DD349 pKa = 3.71LFAQMGAKK357 pKa = 9.89LPNLRR362 pKa = 11.84EE363 pKa = 3.81LDD365 pKa = 4.36LIFDD369 pKa = 4.42GMM371 pKa = 4.03

Molecular weight: 43.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KEK2|A0A1L3KEK2_9VIRU RNA-directed RNA polymerase OS=Hubei sobemo-like virus 30 OX=1923217 PE=4 SV=1
MM1 pKa = 7.43TFLLVSFLVRR11 pKa = 11.84WAKK14 pKa = 10.4HH15 pKa = 3.99INARR19 pKa = 11.84VCALVSAGSEE29 pKa = 3.97GLYY32 pKa = 10.64SVLDD36 pKa = 4.77EE37 pKa = 4.87ISPSPTPEE45 pKa = 3.09IRR47 pKa = 11.84KK48 pKa = 8.47QKK50 pKa = 9.48KK51 pKa = 9.52GKK53 pKa = 9.55VLTVIDD59 pKa = 5.18DD60 pKa = 3.48ILRR63 pKa = 11.84NIDD66 pKa = 3.12FRR68 pKa = 11.84EE69 pKa = 3.88YY70 pKa = 10.54MDD72 pKa = 5.18FDD74 pKa = 4.42WIDD77 pKa = 3.59ALVIAACSVLTLWASYY93 pKa = 9.73MIFRR97 pKa = 11.84VMGRR101 pKa = 11.84NGRR104 pKa = 11.84RR105 pKa = 11.84IVQRR109 pKa = 11.84LRR111 pKa = 11.84GIQNEE116 pKa = 3.81AMIDD120 pKa = 3.63GSAFHH125 pKa = 6.99AGEE128 pKa = 3.93IPKK131 pKa = 9.66FQIGIARR138 pKa = 11.84SKK140 pKa = 10.91LFVDD144 pKa = 3.08VHH146 pKa = 7.42VGFGIRR152 pKa = 11.84SKK154 pKa = 11.34NFLITPRR161 pKa = 11.84HH162 pKa = 4.83VLEE165 pKa = 4.66GVDD168 pKa = 3.81LKK170 pKa = 11.17EE171 pKa = 4.05VLLVGPTGKK180 pKa = 10.6VIVSLCPQPSRR191 pKa = 11.84LVSDD195 pKa = 3.57LTYY198 pKa = 10.39TYY200 pKa = 10.1IEE202 pKa = 4.1SAIWTKK208 pKa = 10.95LGARR212 pKa = 11.84SANLNNTPITTMVEE226 pKa = 4.22CCGKK230 pKa = 10.25DD231 pKa = 3.17GKK233 pKa = 11.49SNGRR237 pKa = 11.84IRR239 pKa = 11.84KK240 pKa = 7.22TNMFGLISYY249 pKa = 7.62TGSTLPGMSGAAYY262 pKa = 10.16VFNNQVQGIHH272 pKa = 6.42KK273 pKa = 9.88GAAGRR278 pKa = 11.84YY279 pKa = 7.84NLGVSSAVLAAEE291 pKa = 4.6LSKK294 pKa = 11.01LCPPTADD301 pKa = 3.29EE302 pKa = 4.84SLPAKK307 pKa = 10.33DD308 pKa = 3.79AEE310 pKa = 4.6DD311 pKa = 3.63YY312 pKa = 11.42VEE314 pKa = 5.78QFQQQNTWTTQQLEE328 pKa = 4.35KK329 pKa = 10.47KK330 pKa = 8.27IQEE333 pKa = 3.85MDD335 pKa = 3.68KK336 pKa = 8.9YY337 pKa = 8.97TGWSTQEE344 pKa = 3.85TMDD347 pKa = 5.09FNQTLNWDD355 pKa = 3.7EE356 pKa = 4.69SSPSTSDD363 pKa = 3.3STSGAATKK371 pKa = 10.45SNPMFVNYY379 pKa = 9.17VAQGILSDD387 pKa = 4.38CKK389 pKa = 10.81DD390 pKa = 3.16IPTVEE395 pKa = 4.16FTSRR399 pKa = 11.84LEE401 pKa = 4.34SLWSQYY407 pKa = 10.55PVILNRR413 pKa = 11.84LCAVEE418 pKa = 5.06KK419 pKa = 10.36EE420 pKa = 4.25LEE422 pKa = 4.44AIKK425 pKa = 10.62APKK428 pKa = 9.33PVANCEE434 pKa = 4.35SQTEE438 pKa = 4.28PEE440 pKa = 4.47VVPEE444 pKa = 3.87ASKK447 pKa = 11.23VVTVVDD453 pKa = 3.48KK454 pKa = 11.17TPFCFLCSKK463 pKa = 10.68HH464 pKa = 5.95FATEE468 pKa = 3.11NGYY471 pKa = 7.57QHH473 pKa = 7.18HH474 pKa = 6.99ISNSKK479 pKa = 8.92KK480 pKa = 8.29HH481 pKa = 5.22TVAEE485 pKa = 4.26SAIPGDD491 pKa = 3.74TGDD494 pKa = 3.28KK495 pKa = 10.43GKK497 pKa = 10.51VVRR500 pKa = 11.84TAGPAPFLGPATTSQKK516 pKa = 10.31RR517 pKa = 11.84KK518 pKa = 8.04KK519 pKa = 8.31TSSSKK524 pKa = 10.41SSGRR528 pKa = 11.84SARR531 pKa = 11.84RR532 pKa = 11.84NQSPPNQGHH541 pKa = 6.75PSEE544 pKa = 4.71MMSSLKK550 pKa = 10.78NIEE553 pKa = 4.54LVLKK557 pKa = 10.88NLVQVMDD564 pKa = 4.08GQKK567 pKa = 10.19SGKK570 pKa = 8.58EE571 pKa = 3.9RR572 pKa = 11.84NN573 pKa = 3.51

Molecular weight: 63.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

944

371

573

472.0

53.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.826 ± 1.082

1.589 ± 0.31

5.826 ± 1.371

5.191 ± 0.369

4.025 ± 0.501

5.932 ± 0.819

2.119 ± 0.35

6.356 ± 0.723

6.462 ± 0.486

9.004 ± 1.242

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.072 ± 0.589

4.449 ± 0.246

4.873 ± 0.176

3.919 ± 0.416

5.085 ± 0.349

8.792 ± 0.592

5.191 ± 1.35

7.309 ± 0.51

2.119 ± 0.677

2.86 ± 0.554
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski