Mycobacterium phage Pollywog

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Cheoctovirus; unclassified Cheoctovirus

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 105 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A411CAV7|A0A411CAV7_9CAUD Uncharacterized protein OS=Mycobacterium phage Pollywog OX=2502448 GN=40 PE=4 SV=1
MM1 pKa = 6.55EE2 pKa = 6.07AKK4 pKa = 10.46YY5 pKa = 8.94KK6 pKa = 10.19TDD8 pKa = 3.1DD9 pKa = 3.21RR10 pKa = 11.84VRR12 pKa = 11.84ILVGGPPEE20 pKa = 4.01NLGRR24 pKa = 11.84IATVSYY30 pKa = 10.61LLEE33 pKa = 5.8SGDD36 pKa = 3.63LVLYY40 pKa = 10.74FGDD43 pKa = 3.54QDD45 pKa = 3.34EE46 pKa = 5.35DD47 pKa = 3.34EE48 pKa = 4.51WGVYY52 pKa = 9.84SPNEE56 pKa = 3.98VEE58 pKa = 4.94LCPTTT63 pKa = 3.68

Molecular weight: 7.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A411CAN1|A0A411CAN1_9CAUD HTH DNA binding domain protein OS=Mycobacterium phage Pollywog OX=2502448 GN=41 PE=4 SV=1
MM1 pKa = 6.68RR2 pKa = 11.84TAYY5 pKa = 9.46KK6 pKa = 10.35VRR8 pKa = 11.84AYY10 pKa = 9.99PDD12 pKa = 3.54AEE14 pKa = 3.94QAALLRR20 pKa = 11.84RR21 pKa = 11.84TFGCVRR27 pKa = 11.84LVWNKK32 pKa = 8.63TLAEE36 pKa = 3.94RR37 pKa = 11.84QQRR40 pKa = 11.84YY41 pKa = 5.17TTEE44 pKa = 4.09QKK46 pKa = 8.79STSYY50 pKa = 11.18KK51 pKa = 8.93EE52 pKa = 3.49TDD54 pKa = 3.15AALSEE59 pKa = 4.27WKK61 pKa = 8.75KK62 pKa = 9.31TEE64 pKa = 3.98DD65 pKa = 3.43LAFLSEE71 pKa = 4.44VSSVPLQQTLRR82 pKa = 11.84HH83 pKa = 4.8QHH85 pKa = 5.1SAFAAFFKK93 pKa = 11.02GLAKK97 pKa = 10.48YY98 pKa = 9.91PRR100 pKa = 11.84FKK102 pKa = 10.64SRR104 pKa = 11.84HH105 pKa = 4.52GRR107 pKa = 11.84QSAHH111 pKa = 4.97FTRR114 pKa = 11.84SAFRR118 pKa = 11.84IKK120 pKa = 10.73DD121 pKa = 3.4GALWLAKK128 pKa = 9.15TATPLRR134 pKa = 11.84IVWTWPGVDD143 pKa = 4.93LAALDD148 pKa = 3.81PTMVIVSRR156 pKa = 11.84EE157 pKa = 3.56PDD159 pKa = 3.33GRR161 pKa = 11.84WFVTFAVDD169 pKa = 4.75QPDD172 pKa = 4.18PQPLPVTGEE181 pKa = 4.2SVGVDD186 pKa = 3.21LGIKK190 pKa = 10.29DD191 pKa = 4.22FATLSTGEE199 pKa = 4.36KK200 pKa = 9.27IANPRR205 pKa = 11.84HH206 pKa = 5.1MARR209 pKa = 11.84HH210 pKa = 4.89EE211 pKa = 4.24RR212 pKa = 11.84GLRR215 pKa = 11.84RR216 pKa = 11.84QQRR219 pKa = 11.84RR220 pKa = 11.84LSRR223 pKa = 11.84MKK225 pKa = 10.38KK226 pKa = 8.77GSKK229 pKa = 9.34NRR231 pKa = 11.84ARR233 pKa = 11.84QRR235 pKa = 11.84VKK237 pKa = 10.29VARR240 pKa = 11.84KK241 pKa = 7.65HH242 pKa = 5.79ARR244 pKa = 11.84VRR246 pKa = 11.84DD247 pKa = 3.57ARR249 pKa = 11.84RR250 pKa = 11.84DD251 pKa = 3.87FLHH254 pKa = 6.02KK255 pKa = 9.64TSTEE259 pKa = 3.7LVRR262 pKa = 11.84RR263 pKa = 11.84FDD265 pKa = 4.03TIAVEE270 pKa = 5.02DD271 pKa = 4.2LAPKK275 pKa = 10.67NMVGNRR281 pKa = 11.84SLAKK285 pKa = 10.31SISEE289 pKa = 4.31CGWGEE294 pKa = 3.82FRR296 pKa = 11.84SMLEE300 pKa = 3.95YY301 pKa = 10.21KK302 pKa = 10.32AKK304 pKa = 9.93KK305 pKa = 8.98AGRR308 pKa = 11.84RR309 pKa = 11.84VAVINRR315 pKa = 11.84WYY317 pKa = 9.65PSSKK321 pKa = 8.31TCSACGHH328 pKa = 6.91LLATLSLGTRR338 pKa = 11.84HH339 pKa = 5.33WTCPDD344 pKa = 3.12CGTRR348 pKa = 11.84HH349 pKa = 6.6DD350 pKa = 4.59RR351 pKa = 11.84DD352 pKa = 3.06INAAKK357 pKa = 10.32NILVAAGLAEE367 pKa = 4.11TQNACGGDD375 pKa = 3.84VRR377 pKa = 11.84PHH379 pKa = 6.14GASHH383 pKa = 6.35RR384 pKa = 11.84QSPVKK389 pKa = 10.34QEE391 pKa = 3.98PSQATARR398 pKa = 11.84IPVLQGGEE406 pKa = 3.87

Molecular weight: 45.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

105

0

105

18543

30

1163

176.6

19.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.602 ± 0.476

1.24 ± 0.155

6.466 ± 0.224

6.029 ± 0.337

2.777 ± 0.138

8.758 ± 0.654

2.087 ± 0.197

4.304 ± 0.165

3.43 ± 0.198

7.501 ± 0.2

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.141 ± 0.129

3.322 ± 0.188

6.132 ± 0.181

3.613 ± 0.198

6.973 ± 0.404

5.932 ± 0.24

6.558 ± 0.265

7.35 ± 0.293

2.173 ± 0.128

2.61 ± 0.125
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski