Photorhabdus temperata subsp. temperata M1021

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Morganellaceae; Photorhabdus; Photorhabdus temperata; Photorhabdus temperata subsp. temperata

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4436 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|T0P0Y7|T0P0Y7_PHOTE PTS system mannose-specific transporter subunit IID OS=Photorhabdus temperata subsp. temperata M1021 OX=1221520 GN=B738_10056 PE=4 SV=1
MM1 pKa = 7.2TGQYY5 pKa = 10.16PFLDD9 pKa = 3.26ILMYY13 pKa = 10.58AYY15 pKa = 10.27FNQDD19 pKa = 2.24FDD21 pKa = 4.84VISGPEE27 pKa = 4.15LDD29 pKa = 5.65DD30 pKa = 5.14VINDD34 pKa = 3.96FLNDD38 pKa = 3.29ASQGMKK44 pKa = 10.06KK45 pKa = 10.64GLIEE49 pKa = 5.0EE50 pKa = 4.35INDD53 pKa = 4.67LIDD56 pKa = 3.75SSEE59 pKa = 4.09DD60 pKa = 3.3VEE62 pKa = 4.54NTFDD66 pKa = 4.23YY67 pKa = 10.52YY68 pKa = 11.74YY69 pKa = 10.83HH70 pKa = 7.41DD71 pKa = 5.1ADD73 pKa = 4.28VLPEE77 pKa = 4.13GWGMTALEE85 pKa = 4.38FLTHH89 pKa = 6.38VSNKK93 pKa = 9.72SQDD96 pKa = 3.74YY97 pKa = 10.47LNKK100 pKa = 9.01HH101 pKa = 5.49TKK103 pKa = 9.07QDD105 pKa = 3.4EE106 pKa = 4.11

Molecular weight: 12.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|T0PD31|T0PD31_PHOTE Flagellar assembly protein FliH OS=Photorhabdus temperata subsp. temperata M1021 OX=1221520 GN=fliH PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.6RR12 pKa = 11.84NRR14 pKa = 11.84SHH16 pKa = 7.16GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.22GRR39 pKa = 11.84ARR41 pKa = 11.84LTVSKK46 pKa = 10.99

Molecular weight: 5.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4436

0

4436

1160171

21

3762

261.5

29.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.207 ± 0.042

1.182 ± 0.014

5.225 ± 0.025

5.97 ± 0.033

3.877 ± 0.025

6.905 ± 0.042

2.34 ± 0.02

6.679 ± 0.038

5.038 ± 0.047

10.569 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.539 ± 0.02

4.442 ± 0.037

4.294 ± 0.029

4.669 ± 0.037

5.334 ± 0.035

6.36 ± 0.034

5.307 ± 0.027

6.492 ± 0.035

1.38 ± 0.015

3.19 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski