Sclerotinia sclerotiorum mycoreovirus 4

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Spinareovirinae; Mycoreovirus; unclassified Mycoreovirus

Average proteome isoelectric point is 6.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A168MTK7|A0A168MTK7_9REOV p10 OS=Sclerotinia sclerotiorum mycoreovirus 4 OX=1840528 PE=4 SV=1
MM1 pKa = 7.45HH2 pKa = 7.97LLTPGLISHH11 pKa = 7.65LSTKK15 pKa = 9.95HH16 pKa = 5.97HH17 pKa = 6.1ATANYY22 pKa = 9.41VPSRR26 pKa = 11.84MAWKK30 pKa = 9.58PYY32 pKa = 7.66QTQLSSNSFVCASAQQLNTASTPQTRR58 pKa = 11.84AQLEE62 pKa = 4.06QRR64 pKa = 11.84VKK66 pKa = 9.39FARR69 pKa = 11.84YY70 pKa = 7.96EE71 pKa = 4.0EE72 pKa = 4.31RR73 pKa = 11.84DD74 pKa = 3.78SIIGYY79 pKa = 10.0ALDD82 pKa = 4.49ASCPLCDD89 pKa = 3.67YY90 pKa = 11.34AEE92 pKa = 4.53SLSDD96 pKa = 3.57TPRR99 pKa = 11.84SPSVGKK105 pKa = 10.29VDD107 pKa = 4.16PSSSSGMGIDD117 pKa = 5.12DD118 pKa = 6.03KK119 pKa = 11.34EE120 pKa = 4.4LSWKK124 pKa = 10.51LDD126 pKa = 3.41LDD128 pKa = 4.35DD129 pKa = 4.28FAKK132 pKa = 10.85AGEE135 pKa = 4.41TVTAAEE141 pKa = 3.84WNEE144 pKa = 4.11RR145 pKa = 11.84FSHH148 pKa = 5.86IPSNFVDD155 pKa = 4.3EE156 pKa = 4.66YY157 pKa = 9.51MLTAASPGIVIVADD171 pKa = 3.78SPAPYY176 pKa = 9.36YY177 pKa = 10.75ASRR180 pKa = 11.84ASKK183 pKa = 10.24FPRR186 pKa = 11.84SRR188 pKa = 11.84QGVIVIPNSINSATSLPNGRR208 pKa = 11.84IIAISTNGHH217 pKa = 5.41EE218 pKa = 4.27ARR220 pKa = 11.84CRR222 pKa = 11.84YY223 pKa = 9.7VDD225 pKa = 3.47LRR227 pKa = 11.84YY228 pKa = 9.64PGRR231 pKa = 11.84KK232 pKa = 8.72RR233 pKa = 11.84LLLEE237 pKa = 4.38VYY239 pKa = 10.72DD240 pKa = 3.88EE241 pKa = 4.58VEE243 pKa = 4.89DD244 pKa = 4.94IEE246 pKa = 5.29EE247 pKa = 4.16EE248 pKa = 4.33DD249 pKa = 3.58PRR251 pKa = 11.84EE252 pKa = 4.25DD253 pKa = 4.74DD254 pKa = 4.51EE255 pKa = 5.57EE256 pKa = 4.03PHH258 pKa = 6.99APLNVMSPILEE269 pKa = 4.01PVIASTSSTSLTGLTLDD286 pKa = 3.83VRR288 pKa = 11.84SASTPPNMPSTSSFSFAEE306 pKa = 4.4DD307 pKa = 3.53PLTNQHH313 pKa = 5.99VPTFDD318 pKa = 3.07HH319 pKa = 6.73RR320 pKa = 11.84RR321 pKa = 11.84AASPLSRR328 pKa = 11.84PDD330 pKa = 3.15TPRR333 pKa = 11.84FMSRR337 pKa = 11.84ARR339 pKa = 11.84CDD341 pKa = 3.12ILLDD345 pKa = 3.87SDD347 pKa = 4.29VSSSMKK353 pKa = 10.51AIASLATSGVITKK366 pKa = 10.46EE367 pKa = 3.97EE368 pKa = 3.89ARR370 pKa = 11.84EE371 pKa = 3.78MLMDD375 pKa = 5.13LGVLL379 pKa = 3.81

Molecular weight: 41.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A168MTI0|A0A168MTI0_9REOV p7 OS=Sclerotinia sclerotiorum mycoreovirus 4 OX=1840528 PE=4 SV=1
MM1 pKa = 7.38NALADD6 pKa = 4.35PNSSNGPPLSDD17 pKa = 3.03VTFPLIRR24 pKa = 11.84RR25 pKa = 11.84SFSHH29 pKa = 5.92ATYY32 pKa = 7.61TTLPLPTDD40 pKa = 3.7IYY42 pKa = 11.49QMLRR46 pKa = 11.84CVEE49 pKa = 4.28VYY51 pKa = 10.66LEE53 pKa = 4.01RR54 pKa = 11.84LYY56 pKa = 10.69TADD59 pKa = 2.84VTRR62 pKa = 11.84YY63 pKa = 9.19VDD65 pKa = 3.37VVSNYY70 pKa = 9.92RR71 pKa = 11.84KK72 pKa = 8.01TSVARR77 pKa = 11.84AVPHH81 pKa = 5.96IFVVNSMEE89 pKa = 4.2KK90 pKa = 10.58FSTSYY95 pKa = 9.62STLLSKK101 pKa = 10.82SPHH104 pKa = 7.16DD105 pKa = 4.0YY106 pKa = 10.54VSSDD110 pKa = 3.22VTVVSNPTSQDD121 pKa = 2.71IIRR124 pKa = 11.84SIRR127 pKa = 11.84AHH129 pKa = 5.65KK130 pKa = 10.22HH131 pKa = 3.51VVCNVVPIGDD141 pKa = 3.77FSDD144 pKa = 5.02AILMPNASISDD155 pKa = 3.7CVKK158 pKa = 8.48YY159 pKa = 10.76LRR161 pKa = 11.84RR162 pKa = 11.84NFHH165 pKa = 6.14ALLRR169 pKa = 11.84RR170 pKa = 11.84LSFGHH175 pKa = 6.37GCYY178 pKa = 10.1SCSNVLQLSLPPWLSVLASGWQRR201 pKa = 11.84FKK203 pKa = 10.66PTGSDD208 pKa = 2.63TDD210 pKa = 3.95FAQFYY215 pKa = 7.28LTPARR220 pKa = 11.84PQLSVINSISDD231 pKa = 3.42LSDD234 pKa = 4.07FIINRR239 pKa = 11.84LQANVDD245 pKa = 3.3VVYY248 pKa = 10.69FDD250 pKa = 6.15DD251 pKa = 4.97VLTTDD256 pKa = 3.43GRR258 pKa = 11.84TIAEE262 pKa = 4.63KK263 pKa = 10.39IRR265 pKa = 11.84ATGAQFNVARR275 pKa = 11.84DD276 pKa = 3.61MTHH279 pKa = 6.76FNIIKK284 pKa = 9.62QRR286 pKa = 11.84YY287 pKa = 8.28LEE289 pKa = 3.94QTSIRR294 pKa = 11.84RR295 pKa = 11.84RR296 pKa = 11.84RR297 pKa = 11.84QHH299 pKa = 6.42KK300 pKa = 10.37KK301 pKa = 9.19EE302 pKa = 3.71LHH304 pKa = 5.72ASDD307 pKa = 3.95RR308 pKa = 11.84KK309 pKa = 9.36QNIVARR315 pKa = 11.84VFPPVYY321 pKa = 10.33LVDD324 pKa = 4.47LSVSIRR330 pKa = 11.84AWNSAMPTQQQRR342 pKa = 11.84RR343 pKa = 11.84LSRR346 pKa = 11.84VYY348 pKa = 10.87SDD350 pKa = 4.68LVNLSKK356 pKa = 10.93RR357 pKa = 11.84PLTVLHH363 pKa = 6.44MIAWNGPSVVSTDD376 pKa = 2.91SDD378 pKa = 3.43VWQDD382 pKa = 3.23FTEE385 pKa = 3.98WLFCVVQNVDD395 pKa = 2.79EE396 pKa = 4.5SMARR400 pKa = 11.84LLTAIPYY407 pKa = 8.59MSVEE411 pKa = 4.13IKK413 pKa = 10.49ISGSGFGTWIDD424 pKa = 3.5LRR426 pKa = 11.84NWTEE430 pKa = 4.05GFSLSKK436 pKa = 10.41ILVGRR441 pKa = 11.84TYY443 pKa = 10.47MVGKK447 pKa = 9.93KK448 pKa = 10.13NSGKK452 pKa = 10.94GMIGKK457 pKa = 8.64LIWRR461 pKa = 11.84LGVPVVDD468 pKa = 3.5SDD470 pKa = 4.06DD471 pKa = 3.7YY472 pKa = 11.99GRR474 pKa = 11.84VLLIAEE480 pKa = 4.21NTGVSLEE487 pKa = 4.19DD488 pKa = 3.29AVRR491 pKa = 11.84CHH493 pKa = 6.59FSRR496 pKa = 11.84SYY498 pKa = 11.52ADD500 pKa = 4.03RR501 pKa = 11.84DD502 pKa = 3.7CAPTLFEE509 pKa = 5.11TIMDD513 pKa = 5.3DD514 pKa = 2.82IVTRR518 pKa = 11.84LSIKK522 pKa = 9.96RR523 pKa = 11.84YY524 pKa = 9.39SIPEE528 pKa = 3.78VDD530 pKa = 4.58HH531 pKa = 6.89PALMMFGHH539 pKa = 7.57AYY541 pKa = 10.45DD542 pKa = 4.53EE543 pKa = 4.18LTKK546 pKa = 10.25KK547 pKa = 10.55YY548 pKa = 10.37KK549 pKa = 10.37YY550 pKa = 10.74ADD552 pKa = 3.64FEE554 pKa = 5.14RR555 pKa = 11.84MVRR558 pKa = 11.84SFISHH563 pKa = 6.5HH564 pKa = 5.83GVRR567 pKa = 11.84APDD570 pKa = 3.68GSLLKK575 pKa = 10.59VDD577 pKa = 4.08DD578 pKa = 4.2RR579 pKa = 11.84FVFSTHH585 pKa = 6.2CSEE588 pKa = 5.02EE589 pKa = 3.95ATQVLGANYY598 pKa = 8.8MFQLSTAIDD607 pKa = 3.85SYY609 pKa = 11.72VGVLLRR615 pKa = 11.84GQHH618 pKa = 6.52DD619 pKa = 3.83NSISEE624 pKa = 4.46LMLAVYY630 pKa = 10.0YY631 pKa = 10.69DD632 pKa = 4.57RR633 pKa = 11.84IHH635 pKa = 7.83VNIFDD640 pKa = 4.57LVPTGVVCTVLRR652 pKa = 11.84TGLARR657 pKa = 11.84LVPKK661 pKa = 10.64AA662 pKa = 3.7

Molecular weight: 74.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12

0

12

7719

269

1360

643.3

71.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.931 ± 0.282

1.555 ± 0.132

6.18 ± 0.334

4.508 ± 0.245

4.431 ± 0.228

6.141 ± 0.356

2.902 ± 0.215

5.247 ± 0.144

2.734 ± 0.294

8.835 ± 0.287

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.902 ± 0.207

3.822 ± 0.16

5.299 ± 0.215

3.187 ± 0.173

6.996 ± 0.278

8.317 ± 0.579

6.27 ± 0.256

8.796 ± 0.505

1.296 ± 0.131

3.64 ± 0.235
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski