Streptomyces sp. TSRI0107

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7155 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Q5L7F5|A0A1Q5L7F5_9ACTN ABC transporter substrate-binding protein OS=Streptomyces sp. TSRI0107 OX=1703942 GN=AMK31_00400 PE=4 SV=1
MM1 pKa = 7.81TDD3 pKa = 3.42DD4 pKa = 3.32QHH6 pKa = 9.61GEE8 pKa = 4.06GTPQGGGRR16 pKa = 11.84WDD18 pKa = 4.22PLPQGDD24 pKa = 4.33YY25 pKa = 11.28DD26 pKa = 4.37DD27 pKa = 4.9GATAFVQLPEE37 pKa = 4.38GGIDD41 pKa = 3.5ALLAASEE48 pKa = 4.73SPLAAPGHH56 pKa = 6.43GYY58 pKa = 9.86VPPPITVTPATTAGSDD74 pKa = 3.59PAATGAWSMPAEE86 pKa = 4.68GAQWPDD92 pKa = 3.61PEE94 pKa = 5.62AVPQQDD100 pKa = 3.24AAHH103 pKa = 6.95DD104 pKa = 3.9RR105 pKa = 11.84FTYY108 pKa = 10.57DD109 pKa = 4.04PGATGQWTYY118 pKa = 11.82AEE120 pKa = 4.31PPEE123 pKa = 4.46TYY125 pKa = 10.18EE126 pKa = 4.24EE127 pKa = 4.14QPEE130 pKa = 4.03ALPAPGHH137 pKa = 6.65DD138 pKa = 3.68VTGQWSIPVAGGDD151 pKa = 3.84LPDD154 pKa = 3.81EE155 pKa = 4.7SGEE158 pKa = 4.22FTTSALVEE166 pKa = 4.23QWGGTPPATLPGGAPAPWATEE187 pKa = 3.99GAGQAWAQQATAGDD201 pKa = 4.43GGRR204 pKa = 11.84HH205 pKa = 5.52PQDD208 pKa = 3.58HH209 pKa = 6.5PQSHH213 pKa = 6.32SEE215 pKa = 3.75AHH217 pKa = 6.49AEE219 pKa = 3.82PTPAHH224 pKa = 5.76GMYY227 pKa = 10.21VGHH230 pKa = 6.97LPEE233 pKa = 6.36AEE235 pKa = 4.37AEE237 pKa = 4.25AEE239 pKa = 4.31SAEE242 pKa = 4.2APAPEE247 pKa = 5.21AGEE250 pKa = 4.12AGEE253 pKa = 4.95AGGAPEE259 pKa = 4.5TPGASDD265 pKa = 3.14TAGAGEE271 pKa = 4.07AVEE274 pKa = 4.31AVSGAVEE281 pKa = 4.35TPEE284 pKa = 4.04GTEE287 pKa = 4.01PTGMAPDD294 pKa = 4.09APTGGEE300 pKa = 4.13SAAEE304 pKa = 4.38GPAEE308 pKa = 4.33AEE310 pKa = 4.23AEE312 pKa = 4.32VLPDD316 pKa = 4.41AEE318 pKa = 4.47PAQAAGTPPPPSDD331 pKa = 3.25AAPEE335 pKa = 4.05QEE337 pKa = 4.84HH338 pKa = 6.56APEE341 pKa = 4.37PAAAPDD347 pKa = 3.67AAEE350 pKa = 5.33DD351 pKa = 4.11PPALPGEE358 pKa = 4.17EE359 pKa = 4.74HH360 pKa = 6.93PLASYY365 pKa = 9.32VLRR368 pKa = 11.84VNGGDD373 pKa = 4.29RR374 pKa = 11.84PVTDD378 pKa = 3.0AWIGEE383 pKa = 4.15SLLYY387 pKa = 10.18VLRR390 pKa = 11.84EE391 pKa = 3.94RR392 pKa = 11.84LGLAGAKK399 pKa = 9.72DD400 pKa = 3.75GCSQGEE406 pKa = 4.12CGACNVQVDD415 pKa = 3.94GRR417 pKa = 11.84LVASCLVPAVTAAGSEE433 pKa = 4.05VRR435 pKa = 11.84TVEE438 pKa = 3.92GLAVDD443 pKa = 4.62GQPSDD448 pKa = 3.61VQRR451 pKa = 11.84ALARR455 pKa = 11.84CGAVQCGFCVPGMAMTVHH473 pKa = 7.27DD474 pKa = 5.16LLEE477 pKa = 4.78GNPAPTEE484 pKa = 4.08LEE486 pKa = 4.08TRR488 pKa = 11.84QALCGNLCRR497 pKa = 11.84CSGYY501 pKa = 10.16RR502 pKa = 11.84AVVDD506 pKa = 3.87AVQEE510 pKa = 4.4VVAEE514 pKa = 4.29RR515 pKa = 11.84DD516 pKa = 2.96AHH518 pKa = 5.75AASDD522 pKa = 3.55AAEE525 pKa = 4.13TDD527 pKa = 3.71EE528 pKa = 5.81ARR530 pKa = 11.84IPHH533 pKa = 5.5QAGPGAGGVNPSAFEE548 pKa = 3.81AFQPHH553 pKa = 7.1DD554 pKa = 3.79PSFGQGPGDD563 pKa = 3.72GQGQGQGQDD572 pKa = 3.28GGQVV576 pKa = 2.88

Molecular weight: 57.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Q5L143|A0A1Q5L143_9ACTN zf-HC2 domain-containing protein OS=Streptomyces sp. TSRI0107 OX=1703942 GN=AMK31_10425 PE=4 SV=1
MM1 pKa = 7.17AAVRR5 pKa = 11.84VVRR8 pKa = 11.84VVPAAPVGRR17 pKa = 11.84VAAASPAVPAAAVAPVVRR35 pKa = 11.84AVAAASPVVRR45 pKa = 11.84VLPAVAAASAAAVAVPASAAVPAVPVAVVARR76 pKa = 11.84RR77 pKa = 11.84ARR79 pKa = 11.84SVARR83 pKa = 11.84VVPRR87 pKa = 11.84VAAASRR93 pKa = 11.84SGRR96 pKa = 11.84GARR99 pKa = 11.84STRR102 pKa = 11.84PCRR105 pKa = 11.84PRR107 pKa = 11.84ASAAA111 pKa = 3.29

Molecular weight: 10.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7155

0

7155

2402202

37

6726

335.7

35.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.696 ± 0.051

0.769 ± 0.006

6.124 ± 0.023

5.864 ± 0.029

2.709 ± 0.015

9.467 ± 0.032

2.309 ± 0.014

3.01 ± 0.02

2.067 ± 0.022

10.441 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.681 ± 0.012

1.698 ± 0.017

6.165 ± 0.026

2.679 ± 0.017

8.19 ± 0.035

4.79 ± 0.025

6.138 ± 0.025

8.549 ± 0.025

1.545 ± 0.012

2.111 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski