Sulfolobus virus Kamchatka 1

Taxonomy: Viruses; Fuselloviridae; Alphafusellovirus; Sulfolobus spindle-shaped virus 9

Average proteome isoelectric point is 7.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 31 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q6TDL3|Q6TDL3_9VIRU ORF B111 OS=Sulfolobus virus Kamchatka 1 OX=248496 PE=4 SV=1
MM1 pKa = 7.8PNYY4 pKa = 10.27LQTSNGYY11 pKa = 9.82LLFVLTASVEE21 pKa = 4.09ANEE24 pKa = 4.24YY25 pKa = 11.11SNGIYY30 pKa = 10.5LGTTYY35 pKa = 11.24LFLQITLDD43 pKa = 3.92FVNSANTYY51 pKa = 9.91EE52 pKa = 4.07ITAQVWTYY60 pKa = 9.6TGTLISYY67 pKa = 8.23TEE69 pKa = 4.21TPITLYY75 pKa = 10.53PNGEE79 pKa = 3.97AFMTLLYY86 pKa = 10.6NYY88 pKa = 10.41NNGNVATGALQEE100 pKa = 4.37GSTVIDD106 pKa = 3.78VYY108 pKa = 11.21FPTSYY113 pKa = 10.73SYY115 pKa = 11.85NSVTYY120 pKa = 10.63NVINTYY126 pKa = 10.86GFTDD130 pKa = 4.01PSGANPSFAIEE141 pKa = 4.12GTYY144 pKa = 9.56TGQPPFSDD152 pKa = 3.8TGWDD156 pKa = 3.84GMWINGAPEE165 pKa = 5.0PINAVAGHH173 pKa = 6.49SSTTDD178 pKa = 3.07VGGTAPIPSYY188 pKa = 10.29YY189 pKa = 10.45AEE191 pKa = 4.38SGLTLFHH198 pKa = 6.8NVFINYY204 pKa = 7.91WYY206 pKa = 10.81NPYY209 pKa = 10.73AWYY212 pKa = 9.73FSIGTPQDD220 pKa = 3.63YY221 pKa = 10.35SAHH224 pKa = 5.72GLNYY228 pKa = 9.25VQLSEE233 pKa = 4.1GQSYY237 pKa = 7.68NTINGLNYY245 pKa = 9.71ALYY248 pKa = 9.55WISPP252 pKa = 3.82

Molecular weight: 27.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q6TDN5|Q6TDN5_9VIRU ORF B83 OS=Sulfolobus virus Kamchatka 1 OX=248496 PE=4 SV=1
MM1 pKa = 6.81MRR3 pKa = 11.84WMSNGLVSPKK13 pKa = 10.17NIRR16 pKa = 11.84RR17 pKa = 11.84NSPRR21 pKa = 11.84FRR23 pKa = 11.84FNYY26 pKa = 9.77AHH28 pKa = 6.59YY29 pKa = 10.08CFTIQKK35 pKa = 9.56VKK37 pKa = 10.18AQEE40 pKa = 3.87RR41 pKa = 11.84EE42 pKa = 4.31TNIKK46 pKa = 8.85PQIHH50 pKa = 6.06RR51 pKa = 11.84VLSHH55 pKa = 6.65RR56 pKa = 11.84GFGISLTRR64 pKa = 11.84RR65 pKa = 11.84ALCLLHH71 pKa = 6.68HH72 pKa = 6.86LRR74 pKa = 11.84NLFYY78 pKa = 11.04GIIDD82 pKa = 3.46

Molecular weight: 9.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

31

0

31

5333

43

793

172.0

19.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.382 ± 0.391

0.694 ± 0.222

3.394 ± 0.469

6.207 ± 0.927

4.744 ± 0.329

6.413 ± 0.631

1.144 ± 0.25

7.65 ± 0.427

7.332 ± 1.106

11.082 ± 0.654

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.1 ± 0.213

5.044 ± 0.542

4.125 ± 0.329

2.925 ± 0.331

4.088 ± 0.671

6.45 ± 0.682

6.469 ± 0.666

7.463 ± 0.399

1.331 ± 0.163

5.963 ± 0.558
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski