Polygonum ringspot tospovirus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Ellioviricetes; Bunyavirales; Tospoviridae; Orthotospovirus

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A4GW61|A4GW61_9VIRU Nucleoprotein OS=Polygonum ringspot tospovirus OX=430606 GN=N PE=3 SV=1
MM1 pKa = 7.07NRR3 pKa = 11.84HH4 pKa = 5.7YY5 pKa = 11.29LLLYY9 pKa = 10.23CLGVYY14 pKa = 10.67LMFTITEE21 pKa = 4.18VFLMNHH27 pKa = 6.42AEE29 pKa = 3.99DD30 pKa = 4.87HH31 pKa = 6.88EE32 pKa = 4.34DD33 pKa = 3.28TKK35 pKa = 11.51VEE37 pKa = 4.14LKK39 pKa = 10.45RR40 pKa = 11.84LKK42 pKa = 10.41DD43 pKa = 3.28RR44 pKa = 11.84YY45 pKa = 10.62KK46 pKa = 10.3IDD48 pKa = 4.01DD49 pKa = 4.57PEE51 pKa = 6.15DD52 pKa = 3.65LLEE55 pKa = 5.83DD56 pKa = 4.21SIKK59 pKa = 10.16DD60 pKa = 3.37TGMVATSGAHH70 pKa = 6.58PSLEE74 pKa = 4.32SKK76 pKa = 9.82TSGRR80 pKa = 11.84SPRR83 pKa = 11.84EE84 pKa = 3.82VTTSSSPVLGPRR96 pKa = 11.84GNFNFSCEE104 pKa = 4.16GFDD107 pKa = 5.08KK108 pKa = 10.72SQCIIKK114 pKa = 10.43SVSDD118 pKa = 4.18FNAHH122 pKa = 4.1YY123 pKa = 10.17QIRR126 pKa = 11.84NLRR129 pKa = 11.84YY130 pKa = 9.01VLACITDD137 pKa = 3.65SEE139 pKa = 5.25KK140 pKa = 10.85IFDD143 pKa = 3.74MCLFDD148 pKa = 5.98SDD150 pKa = 3.23IKK152 pKa = 11.04RR153 pKa = 11.84RR154 pKa = 11.84DD155 pKa = 3.6FLKK158 pKa = 10.85VPVVPVLKK166 pKa = 10.8LEE168 pKa = 4.2NKK170 pKa = 9.7RR171 pKa = 11.84VLEE174 pKa = 4.18VGSKK178 pKa = 10.11FFFVDD183 pKa = 3.56SSNDD187 pKa = 3.49PVPIDD192 pKa = 3.99PNAGMVNATISRR204 pKa = 11.84LSVRR208 pKa = 11.84LSGDD212 pKa = 3.55CVITKK217 pKa = 10.24VSMSSPYY224 pKa = 9.25MIKK227 pKa = 10.1IKK229 pKa = 9.42TAEE232 pKa = 4.2NIGFAVKK239 pKa = 10.27DD240 pKa = 3.73VRR242 pKa = 11.84DD243 pKa = 3.77SQAADD248 pKa = 3.41LQTASGDD255 pKa = 3.11RR256 pKa = 11.84SVYY259 pKa = 10.23FKK261 pKa = 11.19NSEE264 pKa = 3.82LDD266 pKa = 3.44GNHH269 pKa = 6.25FFLCGDD275 pKa = 3.53KK276 pKa = 10.96SSLISKK282 pKa = 9.79VDD284 pKa = 3.42VPVRR288 pKa = 11.84NCVSKK293 pKa = 11.27YY294 pKa = 10.19ADD296 pKa = 3.58EE297 pKa = 4.34PKK299 pKa = 10.65KK300 pKa = 10.79IFFCANFSFFKK311 pKa = 10.32WIFTLLVIAFPVTWLLWKK329 pKa = 9.11TKK331 pKa = 9.32NAFMVWYY338 pKa = 9.11DD339 pKa = 3.36IIGILTYY346 pKa = 10.33PILVLINYY354 pKa = 7.15FWVYY358 pKa = 10.02FPFRR362 pKa = 11.84CKK364 pKa = 10.65VCGNLSFLTHH374 pKa = 6.35TCSKK378 pKa = 10.92LCVCNQCEE386 pKa = 3.96PSKK389 pKa = 10.84EE390 pKa = 4.13HH391 pKa = 7.2AKK393 pKa = 10.16EE394 pKa = 4.17CYY396 pKa = 9.82LFSKK400 pKa = 9.42DD401 pKa = 3.43TQEE404 pKa = 3.72WRR406 pKa = 11.84SLSLIDD412 pKa = 4.66HH413 pKa = 6.48FQFTVNTKK421 pKa = 9.54ISSEE425 pKa = 3.9FLVFLTKK432 pKa = 9.96MLIAAVLISYY442 pKa = 9.73IPSSMALSMQKK453 pKa = 9.67NVCVEE458 pKa = 3.89RR459 pKa = 11.84CYY461 pKa = 10.87YY462 pKa = 10.92SKK464 pKa = 11.01DD465 pKa = 3.53LEE467 pKa = 4.32RR468 pKa = 11.84LTTDD472 pKa = 3.03KK473 pKa = 11.34DD474 pKa = 3.76GSSRR478 pKa = 11.84NAIDD482 pKa = 3.65TCDD485 pKa = 3.84CSIGDD490 pKa = 5.28LITEE494 pKa = 4.43TVYY497 pKa = 10.87RR498 pKa = 11.84GGTPISRR505 pKa = 11.84ATTKK509 pKa = 10.47NDD511 pKa = 3.24CVLSSSACLTSSNQAKK527 pKa = 10.4NLFACRR533 pKa = 11.84NGCNALTTLEE543 pKa = 4.8KK544 pKa = 10.44IPTVKK549 pKa = 10.53YY550 pKa = 8.6SSNYY554 pKa = 9.85RR555 pKa = 11.84GIEE558 pKa = 3.73YY559 pKa = 10.45SGNLTVLKK567 pKa = 10.16IANRR571 pKa = 11.84LRR573 pKa = 11.84KK574 pKa = 10.13GFVDD578 pKa = 3.45NQSEE582 pKa = 4.35VRR584 pKa = 11.84NLEE587 pKa = 4.25SKK589 pKa = 10.26VSKK592 pKa = 10.66DD593 pKa = 3.21LLYY596 pKa = 10.86FKK598 pKa = 10.3NLKK601 pKa = 9.93VDD603 pKa = 4.54DD604 pKa = 4.48VPPSNLMPRR613 pKa = 11.84QSLVFSTEE621 pKa = 3.11VDD623 pKa = 3.03GKK625 pKa = 10.21YY626 pKa = 9.98RR627 pKa = 11.84YY628 pKa = 9.71LIEE631 pKa = 4.11MDD633 pKa = 3.17IKK635 pKa = 11.02KK636 pKa = 8.58EE637 pKa = 4.09TGSVFLLNDD646 pKa = 4.06DD647 pKa = 4.25SSHH650 pKa = 7.44VPMEE654 pKa = 3.82FIIYY658 pKa = 8.5VKK660 pKa = 10.73NVGVDD665 pKa = 3.19YY666 pKa = 10.61DD667 pKa = 3.5IKK669 pKa = 11.09YY670 pKa = 10.11KK671 pKa = 10.97YY672 pKa = 9.3STAKK676 pKa = 10.7VEE678 pKa = 4.28TTVTDD683 pKa = 4.17FLSTCTGTCEE693 pKa = 4.26DD694 pKa = 5.15CKK696 pKa = 9.7TQKK699 pKa = 10.52PLTGRR704 pKa = 11.84HH705 pKa = 5.9DD706 pKa = 3.77FCVQPTSWWGCEE718 pKa = 3.82EE719 pKa = 4.55LGCLAIDD726 pKa = 3.76EE727 pKa = 4.6GAICGHH733 pKa = 5.89CTNVFDD739 pKa = 6.71LSTLVNVYY747 pKa = 9.6QVVQSHH753 pKa = 5.55VAAEE757 pKa = 3.97ICIKK761 pKa = 10.52SVNGYY766 pKa = 9.05DD767 pKa = 4.32CRR769 pKa = 11.84KK770 pKa = 8.75HH771 pKa = 5.23TDD773 pKa = 3.12KK774 pKa = 11.38VPIQTDD780 pKa = 4.42YY781 pKa = 10.94YY782 pKa = 9.64QLDD785 pKa = 3.82MTVDD789 pKa = 3.35LHH791 pKa = 7.06NDD793 pKa = 3.14YY794 pKa = 10.9MSTDD798 pKa = 3.08KK799 pKa = 11.27LFAVNKK805 pKa = 6.17QQKK808 pKa = 9.14VLTGSISDD816 pKa = 4.26LGDD819 pKa = 3.6FSSSAFGHH827 pKa = 5.27PQINIDD833 pKa = 3.99GKK835 pKa = 9.66PLAVPATLSRR845 pKa = 11.84DD846 pKa = 3.48QFSWSCSAVGAKK858 pKa = 9.33SVNIRR863 pKa = 11.84QCGLFSYY870 pKa = 11.14NMIYY874 pKa = 10.98ALTQSRR880 pKa = 11.84DD881 pKa = 3.37FAVLSEE887 pKa = 4.67SEE889 pKa = 4.17NKK891 pKa = 10.6LYY893 pKa = 7.53MTKK896 pKa = 10.82DD897 pKa = 3.41FLVGKK902 pKa = 9.42LKK904 pKa = 11.0VVVDD908 pKa = 3.83MPKK911 pKa = 10.84EE912 pKa = 4.09MFKK915 pKa = 10.83KK916 pKa = 10.2PPSKK920 pKa = 10.17PAISEE925 pKa = 4.31TKK927 pKa = 10.07VNCSGCFKK935 pKa = 10.51CAMGLEE941 pKa = 4.48CDD943 pKa = 4.75LEE945 pKa = 4.48YY946 pKa = 10.84TSDD949 pKa = 3.42TTFSSRR955 pKa = 11.84LEE957 pKa = 3.78IEE959 pKa = 4.27GCSFKK964 pKa = 10.78SDD966 pKa = 3.44QLGSYY971 pKa = 9.83IGPNKK976 pKa = 10.22KK977 pKa = 9.96KK978 pKa = 10.18IKK980 pKa = 9.73AYY982 pKa = 9.98CSEE985 pKa = 4.33SIKK988 pKa = 10.94DD989 pKa = 3.67KK990 pKa = 10.19NIKK993 pKa = 8.72MVAEE997 pKa = 5.09DD998 pKa = 4.68DD999 pKa = 4.5DD1000 pKa = 4.21EE1001 pKa = 6.06LSIEE1005 pKa = 4.17LKK1007 pKa = 9.95IDD1009 pKa = 3.3QVDD1012 pKa = 3.78VVEE1015 pKa = 4.58QDD1017 pKa = 3.47TIINYY1022 pKa = 9.43DD1023 pKa = 3.75DD1024 pKa = 4.93KK1025 pKa = 11.42SAHH1028 pKa = 7.14DD1029 pKa = 4.2EE1030 pKa = 4.52NIHH1033 pKa = 6.92HH1034 pKa = 7.19SDD1036 pKa = 3.19TGLATLWDD1044 pKa = 4.21WIKK1047 pKa = 11.44APFNWVASFFGNFFDD1062 pKa = 3.5IVRR1065 pKa = 11.84VILVIIAICVGIYY1078 pKa = 9.67IISYY1082 pKa = 9.4IYY1084 pKa = 10.61SLSFSYY1090 pKa = 11.04YY1091 pKa = 9.47KK1092 pKa = 10.36EE1093 pKa = 3.75KK1094 pKa = 10.6RR1095 pKa = 11.84NKK1097 pKa = 10.17KK1098 pKa = 9.6IEE1100 pKa = 4.05FDD1102 pKa = 3.79LEE1104 pKa = 4.18EE1105 pKa = 4.29VSQSLLKK1112 pKa = 9.87MSKK1115 pKa = 9.84RR1116 pKa = 11.84DD1117 pKa = 3.3GEE1119 pKa = 4.38RR1120 pKa = 11.84RR1121 pKa = 11.84RR1122 pKa = 11.84KK1123 pKa = 9.97SPPKK1127 pKa = 8.97TFEE1130 pKa = 4.0FPLEE1134 pKa = 4.05FF1135 pKa = 5.44

Molecular weight:
128.51 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A4GW61|A4GW61_9VIRU Nucleoprotein OS=Polygonum ringspot tospovirus OX=430606 GN=N PE=3 SV=1
MM1 pKa = 7.18STTGLSKK8 pKa = 11.15EE9 pKa = 4.16NVEE12 pKa = 4.29KK13 pKa = 10.98LLAGGDD19 pKa = 3.34ADD21 pKa = 4.12VEE23 pKa = 4.3IEE25 pKa = 4.13AEE27 pKa = 4.11EE28 pKa = 3.91TAGFNFKK35 pKa = 10.67QFVTDD40 pKa = 3.55NSKK43 pKa = 10.26MKK45 pKa = 8.56MTFNNGFTILRR56 pKa = 11.84NRR58 pKa = 11.84AAIYY62 pKa = 10.75KK63 pKa = 8.15MVKK66 pKa = 9.76AGHH69 pKa = 6.32FKK71 pKa = 10.68FQGKK75 pKa = 9.99SIVVPSSTSSPGQDD89 pKa = 2.27DD90 pKa = 3.56WTFRR94 pKa = 11.84RR95 pKa = 11.84LEE97 pKa = 3.88GFIRR101 pKa = 11.84AKK103 pKa = 10.26MFMEE107 pKa = 5.64LINMEE112 pKa = 4.33NAVEE116 pKa = 3.92QQKK119 pKa = 9.76MYY121 pKa = 10.96EE122 pKa = 4.29KK123 pKa = 10.53LCEE126 pKa = 3.95LPMVNAYY133 pKa = 9.31GLKK136 pKa = 9.9PSSKK140 pKa = 10.05FDD142 pKa = 3.26PTTARR147 pKa = 11.84VVLTLGGPLPLMASLDD163 pKa = 3.76KK164 pKa = 10.58FAAAAFPLAYY174 pKa = 9.6FQNVKK179 pKa = 10.56KK180 pKa = 10.21EE181 pKa = 3.83ALGINKK187 pKa = 9.37FSTYY191 pKa = 9.74EE192 pKa = 3.86QLCKK196 pKa = 9.76IARR199 pKa = 11.84VMATKK204 pKa = 10.54EE205 pKa = 3.97FTFTGASKK213 pKa = 10.88AIFEE217 pKa = 4.32EE218 pKa = 4.76TIKK221 pKa = 10.63ILNDD225 pKa = 3.62CTPGTAGAASLNKK238 pKa = 9.94FNEE241 pKa = 4.15QIKK244 pKa = 10.54ALEE247 pKa = 4.02AVFGKK252 pKa = 10.36IVDD255 pKa = 4.18DD256 pKa = 4.45NNAGSSKK263 pKa = 9.96PRR265 pKa = 11.84PASKK269 pKa = 10.41KK270 pKa = 9.06NQGFF274 pKa = 3.44

Molecular weight:
30.17 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

5036

274

2876

1007.2

114.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.527 ± 1.034

2.264 ± 0.454

6.215 ± 0.399

6.334 ± 0.454

5.242 ± 0.301

4.21 ± 0.461

1.728 ± 0.185

7.168 ± 0.316

8.817 ± 0.324

9.015 ± 0.717

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.415 ± 0.579

6.056 ± 0.447

2.859 ± 0.405

2.562 ± 0.081

3.594 ± 0.179

9.373 ± 0.484

5.977 ± 0.082

5.997 ± 0.629

0.774 ± 0.154

3.872 ± 0.455

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski