Burkholderia multivorans (strain ATCC 17616 / 249)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiaceae; Burkholderia; Burkholderia cepacia complex; Burkholderia multivorans

Average proteome isoelectric point is 6.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6040 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0H3KJQ0|A0A0H3KJQ0_BURM1 Permease of the major facilitator superfamily OS=Burkholderia multivorans (strain ATCC 17616 / 249) OX=395019 GN=BMULJ_01715 PE=4 SV=1
MM1 pKa = 7.36TFSLNPSEE9 pKa = 5.42LFDD12 pKa = 4.94GFDD15 pKa = 3.78SFDD18 pKa = 3.6GFDD21 pKa = 4.19GFDD24 pKa = 3.5EE25 pKa = 4.72PMHH28 pKa = 6.53DD29 pKa = 3.31HH30 pKa = 7.19AGTPEE35 pKa = 3.96EE36 pKa = 4.06AHH38 pKa = 5.23VTSYY42 pKa = 10.98QAVLQEE48 pKa = 4.25PMVSLVGSVDD58 pKa = 3.67RR59 pKa = 11.84PDD61 pKa = 3.5VASVAILGYY70 pKa = 10.35NN71 pKa = 3.21

Molecular weight: 7.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0H3KBX0|A0A0H3KBX0_BURM1 DNA polymerase III subunit delta OS=Burkholderia multivorans (strain ATCC 17616 / 249) OX=395019 GN=holA PE=4 SV=1
MM1 pKa = 7.98PFRR4 pKa = 11.84LTSRR8 pKa = 11.84LSRR11 pKa = 11.84AAKK14 pKa = 9.58RR15 pKa = 11.84VVPSAPARR23 pKa = 11.84PLRR26 pKa = 11.84HH27 pKa = 5.45HH28 pKa = 6.27RR29 pKa = 11.84TRR31 pKa = 11.84VRR33 pKa = 11.84ALAEE37 pKa = 3.88RR38 pKa = 11.84THH40 pKa = 6.33ALSRR44 pKa = 11.84LDD46 pKa = 5.1GIRR49 pKa = 11.84AWFHH53 pKa = 6.5AFLSLMSAQALARR66 pKa = 11.84RR67 pKa = 11.84AGLRR71 pKa = 11.84SLLQKK76 pKa = 10.34PSSLRR81 pKa = 11.84ALALRR86 pKa = 11.84RR87 pKa = 11.84AQAPQRR93 pKa = 11.84RR94 pKa = 11.84VNRR97 pKa = 11.84PRR99 pKa = 11.84RR100 pKa = 11.84LAASNGWFGFGGRR113 pKa = 4.19

Molecular weight: 12.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6040

0

6040

1951710

25

4531

323.1

34.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.854 ± 0.05

0.932 ± 0.012

5.801 ± 0.032

4.723 ± 0.03

3.599 ± 0.021

8.194 ± 0.053

2.323 ± 0.017

4.52 ± 0.024

2.828 ± 0.028

10.137 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.237 ± 0.014

2.528 ± 0.022

5.295 ± 0.025

3.33 ± 0.019

7.486 ± 0.041

5.169 ± 0.04

5.422 ± 0.042

7.788 ± 0.031

1.399 ± 0.015

2.435 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski