Staphylococcus aureus (strain NCTC 8325 / PS 47)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Staphylococcaceae; Staphylococcus; Staphylococcus aureus

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2889 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q2FV95|Q2FV95_STAA8 L-serine dehydratase OS=Staphylococcus aureus (strain NCTC 8325 / PS 47) OX=93061 GN=SAOUHSC_02839 PE=3 SV=1
MM1 pKa = 7.03SQEE4 pKa = 3.83ILNVEE9 pKa = 4.37GMSCGHH15 pKa = 6.67CKK17 pKa = 10.33SAVEE21 pKa = 4.15SALNNIDD28 pKa = 3.49GVTSADD34 pKa = 3.34VNLEE38 pKa = 3.88NGQVSVQYY46 pKa = 10.57DD47 pKa = 3.6DD48 pKa = 4.83SKK50 pKa = 11.42VAVSQMKK57 pKa = 10.25DD58 pKa = 3.06AIEE61 pKa = 4.25DD62 pKa = 3.05QGYY65 pKa = 10.58DD66 pKa = 3.4VVV68 pKa = 4.31

Molecular weight: 7.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q2FUQ1|RNPA_STAA8 Ribonuclease P protein component OS=Staphylococcus aureus (strain NCTC 8325 / PS 47) OX=93061 GN=rnpA PE=1 SV=1
MM1 pKa = 7.49VKK3 pKa = 9.1RR4 pKa = 11.84TYY6 pKa = 10.35QPNKK10 pKa = 8.16RR11 pKa = 11.84KK12 pKa = 9.54HH13 pKa = 5.99SKK15 pKa = 8.87VHH17 pKa = 5.77GFRR20 pKa = 11.84KK21 pKa = 10.02RR22 pKa = 11.84MSTKK26 pKa = 10.18NGRR29 pKa = 11.84KK30 pKa = 8.49VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.05GRR40 pKa = 11.84KK41 pKa = 8.7VLSAA45 pKa = 4.05

Molecular weight: 5.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2889

0

2889

797397

20

9535

276.0

31.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.401 ± 0.095

0.644 ± 0.016

5.812 ± 0.053

6.499 ± 0.067

4.521 ± 0.057

6.032 ± 0.054

2.3 ± 0.027

8.657 ± 0.078

7.547 ± 0.058

9.142 ± 0.072

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.665 ± 0.033

5.685 ± 0.095

3.212 ± 0.034

4.126 ± 0.076

3.502 ± 0.037

6.105 ± 0.097

5.796 ± 0.071

6.681 ± 0.04

0.756 ± 0.019

3.917 ± 0.044
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski