Sphingomonas sp. MM-1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingomonas; unclassified Sphingomonas

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4252 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M4RZT8|M4RZT8_9SPHN Alcohol dehydrogenase OS=Sphingomonas sp. MM-1 OX=745310 GN=G432_02300 PE=3 SV=1
MM1 pKa = 7.08QFLVPDD7 pKa = 4.3MTCGSCVPHH16 pKa = 5.78VTDD19 pKa = 6.51AIAKK23 pKa = 8.23VDD25 pKa = 3.71PGAVVEE31 pKa = 4.46ADD33 pKa = 3.54TVARR37 pKa = 11.84TITVTTSASQQEE49 pKa = 4.29IEE51 pKa = 4.8EE52 pKa = 4.43ALTDD56 pKa = 3.93DD57 pKa = 5.6GYY59 pKa = 10.34PATAII64 pKa = 3.91

Molecular weight: 6.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M4SKR8|M4SKR8_9SPHN Phospholipase A1 OS=Sphingomonas sp. MM-1 OX=745310 GN=G432_17195 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSRR9 pKa = 11.84LVRR12 pKa = 11.84KK13 pKa = 8.99RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.7GFRR19 pKa = 11.84SRR21 pKa = 11.84SATPGGRR28 pKa = 11.84KK29 pKa = 9.04VLAARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 6.76TLSAA44 pKa = 4.22

Molecular weight: 5.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4252

0

4252

1340665

36

4234

315.3

33.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.144 ± 0.062

0.722 ± 0.013

6.063 ± 0.028

5.388 ± 0.039

3.447 ± 0.022

9.14 ± 0.057

1.967 ± 0.019

5.119 ± 0.027

2.731 ± 0.028

9.867 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.362 ± 0.019

2.411 ± 0.026

5.438 ± 0.033

2.925 ± 0.022

7.815 ± 0.042

4.869 ± 0.028

5.009 ± 0.035

7.003 ± 0.031

1.418 ± 0.016

2.161 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski