Metarhizium album (strain ARSEF 1941)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Hypocreomycetidae; Hypocreales; Clavicipitaceae; Metarhizium; Metarhizium album

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8389 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B2WEE3|A0A0B2WEE3_METAS Uncharacterized protein OS=Metarhizium album (strain ARSEF 1941) OX=1081103 GN=MAM_07953 PE=4 SV=1
MM1 pKa = 7.63FDD3 pKa = 5.01DD4 pKa = 4.95EE5 pKa = 4.91NLSIYY10 pKa = 10.65DD11 pKa = 3.91EE12 pKa = 4.49IEE14 pKa = 4.35IEE16 pKa = 5.56DD17 pKa = 3.67MTFDD21 pKa = 4.08EE22 pKa = 5.15SLQLYY27 pKa = 9.1HH28 pKa = 6.46YY29 pKa = 7.68PCPCGDD35 pKa = 3.77RR36 pKa = 11.84FQIALDD42 pKa = 3.95DD43 pKa = 4.77LRR45 pKa = 11.84DD46 pKa = 3.68EE47 pKa = 4.38QDD49 pKa = 2.94IAVCPSCSLMVRR61 pKa = 11.84VIFDD65 pKa = 4.66LDD67 pKa = 4.31DD68 pKa = 4.16LPKK71 pKa = 10.42PPPAGSAAQAPPIAAA86 pKa = 4.08

Molecular weight: 9.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B2WNK5|A0A0B2WNK5_METAS Uncharacterized protein OS=Metarhizium album (strain ARSEF 1941) OX=1081103 GN=MAM_06940 PE=4 SV=1
MM1 pKa = 7.5SAPLRR6 pKa = 11.84LPNTLPARR14 pKa = 11.84PLRR17 pKa = 11.84RR18 pKa = 11.84PVTPSSSISISISISISVSVAIVVVVVVVVVVSLLSLLHH57 pKa = 6.29RR58 pKa = 11.84RR59 pKa = 11.84SAGAALVRR67 pKa = 11.84GVNGAITRR75 pKa = 11.84GNRR78 pKa = 11.84VSVLISRR85 pKa = 11.84RR86 pKa = 11.84RR87 pKa = 11.84FPLVSSSAAAGG98 pKa = 3.41

Molecular weight: 10.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8389

0

8389

4223730

50

10302

503.5

55.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.312 ± 0.026

1.264 ± 0.011

5.948 ± 0.019

5.941 ± 0.028

3.512 ± 0.015

7.147 ± 0.025

2.465 ± 0.011

4.368 ± 0.015

4.701 ± 0.024

8.781 ± 0.027

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.208 ± 0.01

3.421 ± 0.013

6.109 ± 0.027

4.04 ± 0.021

6.661 ± 0.026

8.149 ± 0.028

5.674 ± 0.018

6.346 ± 0.018

1.407 ± 0.011

2.544 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski