Rathayibacter phage NCPPB3778

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 5.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 77 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1P8VVC7|A0A1P8VVC7_9CAUD Uncharacterized protein OS=Rathayibacter phage NCPPB3778 OX=1909694 GN=NCPPB3778_39 PE=4 SV=1
MM1 pKa = 7.62MITDD5 pKa = 3.73EE6 pKa = 5.0FIRR9 pKa = 11.84NIIIDD14 pKa = 3.92TYY16 pKa = 10.97DD17 pKa = 3.22ASEE20 pKa = 4.24VADD23 pKa = 3.68AADD26 pKa = 4.11RR27 pKa = 11.84YY28 pKa = 10.55DD29 pKa = 3.41YY30 pKa = 11.71ARR32 pKa = 11.84ILVAGANILDD42 pKa = 4.12SLLKK46 pKa = 9.88ATLSEE51 pKa = 4.49SFDD54 pKa = 3.61NPAVSDD60 pKa = 3.42VDD62 pKa = 4.32GIKK65 pKa = 10.64NLLMAHH71 pKa = 5.38SCEE74 pKa = 4.57VEE76 pKa = 4.13AEE78 pKa = 3.92DD79 pKa = 6.58DD80 pKa = 3.42ITLLHH85 pKa = 6.44SLAHH89 pKa = 7.45AITCLHH95 pKa = 7.47PDD97 pKa = 3.08RR98 pKa = 11.84LLGYY102 pKa = 10.51GEE104 pKa = 5.32DD105 pKa = 3.84FGIDD109 pKa = 3.61DD110 pKa = 4.45EE111 pKa = 4.8VASS114 pKa = 4.19

Molecular weight: 12.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1P8VV48|A0A1P8VV48_9CAUD Uncharacterized protein OS=Rathayibacter phage NCPPB3778 OX=1909694 GN=NCPPB3778_44 PE=4 SV=1
MM1 pKa = 6.69TAKK4 pKa = 10.23NQRR7 pKa = 11.84DD8 pKa = 4.18GKK10 pKa = 8.45GHH12 pKa = 6.16RR13 pKa = 11.84AYY15 pKa = 10.59RR16 pKa = 11.84RR17 pKa = 11.84LQAALKK23 pKa = 10.45RR24 pKa = 11.84RR25 pKa = 11.84TANQNLPCWLCGQFIDD41 pKa = 3.81TTLPAGHH48 pKa = 6.76RR49 pKa = 11.84MSFSADD55 pKa = 3.06HH56 pKa = 5.77VQALATGGDD65 pKa = 3.68LVKK68 pKa = 11.15NEE70 pKa = 4.82LRR72 pKa = 11.84PSHH75 pKa = 6.58LSCNASRR82 pKa = 11.84GIGSTADD89 pKa = 2.58IWGATT94 pKa = 3.59

Molecular weight: 10.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

77

0

77

13651

37

1062

177.3

19.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.01 ± 0.468

0.418 ± 0.083

6.527 ± 0.377

5.589 ± 0.323

3.011 ± 0.181

8.549 ± 0.476

1.743 ± 0.172

5.934 ± 0.255

3.831 ± 0.235

8.336 ± 0.374

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.11 ± 0.106

2.85 ± 0.233

4.659 ± 0.247

2.923 ± 0.18

5.934 ± 0.344

7.86 ± 0.302

6.6 ± 0.359

7.553 ± 0.289

1.648 ± 0.192

2.916 ± 0.3
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski