Dishui Lake virophage 2

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Preplasmiviricota; Maveriviricetes; Priklausovirales; Lavidaviridae; unclassified Lavidaviridae

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 19 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G6XN33|A0A6G6XN33_9VIRU Uncharacterized protein OS=Dishui Lake virophage 2 OX=2704064 PE=4 SV=1
MM1 pKa = 7.26ATNSSFGYY9 pKa = 10.07LPVGSIIYY17 pKa = 9.5YY18 pKa = 10.01AGNGEE23 pKa = 4.06IPSTFLRR30 pKa = 11.84CDD32 pKa = 2.96GTNYY36 pKa = 10.23DD37 pKa = 3.77RR38 pKa = 11.84EE39 pKa = 4.56TYY41 pKa = 10.56SVLFDD46 pKa = 3.9VLGTSYY52 pKa = 11.53GSTSATNFKK61 pKa = 10.92VPDD64 pKa = 4.92LINYY68 pKa = 8.01YY69 pKa = 8.98YY70 pKa = 10.17IKK72 pKa = 10.8GGAQSSGVPSQAVIDD87 pKa = 4.1VPPFLLNSATLPSLAQGNFVFDD109 pKa = 3.78SWNLTANINNGVWFSNSTPSVDD131 pKa = 3.73VVPSGSDD138 pKa = 3.17DD139 pKa = 3.63TVKK142 pKa = 11.03ANSTDD147 pKa = 3.37QNNYY151 pKa = 7.94TGAVTGGTIGFTNPAQEE168 pKa = 4.77TIDD171 pKa = 4.19LAPDD175 pKa = 3.39PTTTVEE181 pKa = 4.17LQALSLIPLIKK192 pKa = 10.02AWYY195 pKa = 9.3DD196 pKa = 3.55FLPPTYY202 pKa = 9.81TPLPVSTDD210 pKa = 2.71SGIRR214 pKa = 11.84VDD216 pKa = 4.78KK217 pKa = 11.32VNEE220 pKa = 4.05TEE222 pKa = 4.27YY223 pKa = 8.58TTNPTGIYY231 pKa = 10.13LDD233 pKa = 4.81DD234 pKa = 4.53PQLSGFVFEE243 pKa = 5.65KK244 pKa = 9.76PTFF247 pKa = 3.68

Molecular weight: 26.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G6XME2|A0A6G6XME2_9VIRU Collar domain-containing protein OS=Dishui Lake virophage 2 OX=2704064 PE=4 SV=1
MM1 pKa = 7.12PQANITYY8 pKa = 8.74DD9 pKa = 2.89IPYY12 pKa = 8.33NRR14 pKa = 11.84KK15 pKa = 8.2MVDD18 pKa = 2.73ILRR21 pKa = 11.84EE22 pKa = 3.79MDD24 pKa = 3.21EE25 pKa = 4.2KK26 pKa = 10.69HH27 pKa = 5.12WRR29 pKa = 11.84KK30 pKa = 10.32AGDD33 pKa = 3.31AYY35 pKa = 10.73APTMFSEE42 pKa = 4.47KK43 pKa = 10.44LGNFHH48 pKa = 6.62GAKK51 pKa = 9.8IGGGSPANQQYY62 pKa = 9.62IHH64 pKa = 6.81SGNSPAYY71 pKa = 9.23PPVNMNSGMAVSSGGMYY88 pKa = 10.21SGIDD92 pKa = 3.37GAVGGKK98 pKa = 10.15YY99 pKa = 10.45SVDD102 pKa = 3.13KK103 pKa = 10.98FVGDD107 pKa = 3.96FKK109 pKa = 11.43KK110 pKa = 10.68VGKK113 pKa = 9.58LLKK116 pKa = 10.12PVAKK120 pKa = 10.07PILTALTNKK129 pKa = 9.8AVAKK133 pKa = 10.5LGGFSEE139 pKa = 5.13LMTGCGRR146 pKa = 11.84PAPKK150 pKa = 10.67NKK152 pKa = 9.28MDD154 pKa = 3.57VVDD157 pKa = 4.32FLKK160 pKa = 10.88PLGAKK165 pKa = 9.74KK166 pKa = 10.08SHH168 pKa = 5.85SLNRR172 pKa = 11.84LHH174 pKa = 7.0EE175 pKa = 4.22LVAPYY180 pKa = 10.55RR181 pKa = 11.84MEE183 pKa = 4.21GGKK186 pKa = 10.62YY187 pKa = 10.27SVDD190 pKa = 2.79KK191 pKa = 10.41FVRR194 pKa = 11.84DD195 pKa = 3.92FGKK198 pKa = 9.92IGKK201 pKa = 9.07LIKK204 pKa = 10.13PVAKK208 pKa = 10.06PILKK212 pKa = 10.34ALTDD216 pKa = 3.6KK217 pKa = 11.22AVAKK221 pKa = 9.23VTGAGMDD228 pKa = 3.58EE229 pKa = 4.6EE230 pKa = 5.22IEE232 pKa = 4.14LVRR235 pKa = 11.84PMEE238 pKa = 4.03MAEE241 pKa = 4.15TKK243 pKa = 10.83GKK245 pKa = 9.91GKK247 pKa = 10.55KK248 pKa = 8.87GGKK251 pKa = 9.22YY252 pKa = 10.42SVDD255 pKa = 3.15KK256 pKa = 10.6FVKK259 pKa = 10.49DD260 pKa = 3.77FGKK263 pKa = 10.03IGKK266 pKa = 9.07LIKK269 pKa = 10.07PVAKK273 pKa = 10.01PIVKK277 pKa = 10.27ALTDD281 pKa = 3.54KK282 pKa = 11.18AVAKK286 pKa = 10.36IGGGGGRR293 pKa = 11.84AKK295 pKa = 10.32RR296 pKa = 11.84AEE298 pKa = 3.98IVKK301 pKa = 10.36KK302 pKa = 10.86VMAEE306 pKa = 3.71KK307 pKa = 10.2GMKK310 pKa = 9.32MIEE313 pKa = 3.49ASKK316 pKa = 9.99YY317 pKa = 8.27VKK319 pKa = 10.34EE320 pKa = 4.26HH321 pKa = 5.73GLYY324 pKa = 10.57

Molecular weight: 34.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

19

0

19

6250

107

792

328.9

36.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.208 ± 0.472

1.088 ± 0.258

5.808 ± 0.35

5.936 ± 0.895

4.416 ± 0.378

6.608 ± 0.619

1.152 ± 0.213

6.496 ± 0.383

8.368 ± 1.286

8.416 ± 0.437

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.16 ± 0.323

6.304 ± 0.598

4.624 ± 0.485

3.6 ± 0.373

3.616 ± 0.397

7.024 ± 0.766

6.848 ± 0.651

5.728 ± 0.362

1.232 ± 0.21

4.368 ± 0.311
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski