Escherichia phage JN02

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Tevenvirinae; Mosigvirus; unclassified Mosigvirus

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 172 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G8AKT9|A0A7G8AKT9_9CAUD Uncharacterized protein OS=Escherichia phage JN02 OX=2759202 PE=4 SV=1
MM1 pKa = 7.58TYY3 pKa = 10.93SLNLKK8 pKa = 9.99DD9 pKa = 4.0FQDD12 pKa = 3.95VYY14 pKa = 10.31EE15 pKa = 4.4VCANEE20 pKa = 4.74LGPDD24 pKa = 3.4APTIIEE30 pKa = 4.28VLNVLPSSLAYY41 pKa = 9.31EE42 pKa = 4.32AKK44 pKa = 9.87SWGWNDD50 pKa = 3.24TVVRR54 pKa = 11.84DD55 pKa = 4.06DD56 pKa = 6.05LYY58 pKa = 11.98VLMNDD63 pKa = 3.32MMVKK67 pKa = 9.96KK68 pKa = 10.05AEE70 pKa = 4.14PVAPMVTITVEE81 pKa = 4.0EE82 pKa = 4.42YY83 pKa = 10.93NRR85 pKa = 11.84LQAIEE90 pKa = 4.06EE91 pKa = 4.25LLWNIEE97 pKa = 3.93CDD99 pKa = 3.61LPSGLEE105 pKa = 3.59SWIDD109 pKa = 3.54YY110 pKa = 11.02EE111 pKa = 4.54EE112 pKa = 4.29LNKK115 pKa = 10.42LRR117 pKa = 11.84GG118 pKa = 3.47

Molecular weight: 13.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G8AL58|A0A7G8AL58_9CAUD DNA endonuclease IV OS=Escherichia phage JN02 OX=2759202 PE=4 SV=1
MM1 pKa = 7.53SKK3 pKa = 10.8YY4 pKa = 9.31LTRR7 pKa = 11.84KK8 pKa = 10.03DD9 pKa = 3.41LLAVGGEE16 pKa = 4.3VVAVVRR22 pKa = 11.84NGEE25 pKa = 4.15YY26 pKa = 10.39GSEE29 pKa = 3.98VSKK32 pKa = 10.5EE33 pKa = 3.68FRR35 pKa = 11.84SRR37 pKa = 11.84EE38 pKa = 3.58GFYY41 pKa = 10.88FFVKK45 pKa = 10.66GSSDD49 pKa = 2.92WRR51 pKa = 11.84QVAARR56 pKa = 11.84FFVGRR61 pKa = 11.84QRR63 pKa = 11.84SKK65 pKa = 10.99QGLDD69 pKa = 3.53AILSHH74 pKa = 6.36IRR76 pKa = 11.84QGRR79 pKa = 11.84SQLARR84 pKa = 11.84TMGTNNIEE92 pKa = 3.81YY93 pKa = 10.63DD94 pKa = 4.14VIFVAAKK101 pKa = 10.13NMKK104 pKa = 9.57PLTTGYY110 pKa = 11.21GKK112 pKa = 10.77GQLALAFTRR121 pKa = 11.84NHH123 pKa = 4.89TSEE126 pKa = 4.22YY127 pKa = 8.73QTLTEE132 pKa = 4.18MNRR135 pKa = 11.84LLADD139 pKa = 3.24NFKK142 pKa = 11.02FILQSYY148 pKa = 8.66

Molecular weight: 16.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

171

1

172

45334

99

1291

263.6

29.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.851 ± 0.176

1.032 ± 0.073

6.24 ± 0.121

6.953 ± 0.186

4.334 ± 0.11

6.278 ± 0.195

1.687 ± 0.089

7.193 ± 0.14

7.368 ± 0.239

7.445 ± 0.129

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.55 ± 0.11

5.687 ± 0.119

3.664 ± 0.103

3.412 ± 0.116

4.365 ± 0.129

6.428 ± 0.136

6.225 ± 0.243

6.721 ± 0.136

1.341 ± 0.064

4.224 ± 0.137
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski