Phytophthora parasitica (strain INRA-310)

Taxonomy: cellular organisms; Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae; Phytophthora; Phytophthora parasitica

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 26438 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W2Q904|W2Q904_PHYPN FYVE-type domain-containing protein OS=Phytophthora parasitica (strain INRA-310) OX=761204 GN=PPTG_11262 PE=4 SV=1
MM1 pKa = 7.07QVFGVTALVFLALQGSVQAVQNAITCTTTADD32 pKa = 4.08CAWGEE37 pKa = 4.35TCVAGDD43 pKa = 4.38ADD45 pKa = 4.21TSVQACVAPTVCGGSSMGNCPSDD68 pKa = 3.53DD69 pKa = 3.83TGKK72 pKa = 10.26LACLWRR78 pKa = 11.84PFNDD82 pKa = 3.49CSEE85 pKa = 4.19GCAILNGNKK94 pKa = 10.11GIYY97 pKa = 9.36KK98 pKa = 9.7CVSITRR104 pKa = 11.84CDD106 pKa = 3.2AYY108 pKa = 11.34YY109 pKa = 10.86GGSKK113 pKa = 10.56CSDD116 pKa = 3.33GCSVNGVRR124 pKa = 11.84CNGQGSCNMMSSNADD139 pKa = 3.18GTPVFGCTCDD149 pKa = 3.06NGYY152 pKa = 10.63SGEE155 pKa = 4.16KK156 pKa = 10.23CEE158 pKa = 4.41NAPGSTNTSSTDD170 pKa = 3.12TDD172 pKa = 4.45DD173 pKa = 5.33EE174 pKa = 5.0DD175 pKa = 4.87SFWGALDD182 pKa = 5.35DD183 pKa = 5.92GSDD186 pKa = 4.48DD187 pKa = 4.07DD188 pKa = 4.86ASKK191 pKa = 11.23NGSDD195 pKa = 5.13DD196 pKa = 4.78ANADD200 pKa = 3.74DD201 pKa = 5.94SGTSDD206 pKa = 5.21DD207 pKa = 4.56KK208 pKa = 11.59STDD211 pKa = 3.3ASDD214 pKa = 5.54DD215 pKa = 3.73SATDD219 pKa = 3.38EE220 pKa = 4.69SSTDD224 pKa = 3.33TSDD227 pKa = 5.54DD228 pKa = 3.51SSTDD232 pKa = 3.34ADD234 pKa = 3.91QSTTDD239 pKa = 4.91ASDD242 pKa = 3.3EE243 pKa = 4.63SNADD247 pKa = 3.72DD248 pKa = 4.71NAEE251 pKa = 4.43DD252 pKa = 4.61DD253 pKa = 4.38SASSSSSASSSGEE266 pKa = 3.78LANSEE271 pKa = 4.76SVSSTSRR278 pKa = 11.84SGIRR282 pKa = 11.84PGVLILVLVMTAFFLVGTVLLVAYY306 pKa = 9.47SRR308 pKa = 11.84RR309 pKa = 11.84RR310 pKa = 11.84KK311 pKa = 7.81QQEE314 pKa = 3.7EE315 pKa = 4.23EE316 pKa = 4.47EE317 pKa = 4.48YY318 pKa = 11.43ANALASTQGVGAVGARR334 pKa = 11.84DD335 pKa = 3.4LAAGHH340 pKa = 6.21TGRR343 pKa = 11.84TPRR346 pKa = 11.84SNIARR351 pKa = 11.84MM352 pKa = 3.51

Molecular weight: 35.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W2PB64|W2PB64_PHYPN Uncharacterized protein OS=Phytophthora parasitica (strain INRA-310) OX=761204 GN=PPTG_19898 PE=4 SV=1
PP1 pKa = 6.95RR2 pKa = 11.84TPRR5 pKa = 11.84PRR7 pKa = 11.84NLVIRR12 pKa = 11.84RR13 pKa = 11.84SLLRR17 pKa = 11.84SRR19 pKa = 11.84RR20 pKa = 11.84MSQLTLPPMHH30 pKa = 7.24PPMPLLRR37 pKa = 11.84RR38 pKa = 11.84LPTHH42 pKa = 6.83PLMHH46 pKa = 6.71LQMPPPMLQLTRR58 pKa = 11.84LPMRR62 pKa = 11.84QLMHH66 pKa = 7.36RR67 pKa = 11.84LMPQLTLLPMCRR79 pKa = 11.84PTRR82 pKa = 11.84RR83 pKa = 11.84QTSPPMPLLRR93 pKa = 11.84RR94 pKa = 11.84LPTHH98 pKa = 6.89PLMPPPMLQLTRR110 pKa = 11.84LPMRR114 pKa = 11.84QLMRR118 pKa = 11.84RR119 pKa = 11.84LMLQLTLLPMRR130 pKa = 11.84PPMRR134 pKa = 11.84LRR136 pKa = 11.84TRR138 pKa = 11.84PPMLLLRR145 pKa = 11.84RR146 pKa = 11.84LPTHH150 pKa = 6.89PLMPPPMLLLRR161 pKa = 11.84RR162 pKa = 11.84LPTHH166 pKa = 6.85PPMPPPMLQLTRR178 pKa = 11.84RR179 pKa = 11.84LILQLMCLPKK189 pKa = 10.4HH190 pKa = 6.06PLMLQLTRR198 pKa = 11.84PPMRR202 pKa = 11.84RR203 pKa = 11.84PTNLQHH209 pKa = 7.19PVTTKK214 pKa = 10.66VLTVLEE220 pKa = 4.37WRR222 pKa = 11.84TT223 pKa = 3.31

Molecular weight: 26.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

23004

3434

26438

10900847

30

11823

412.3

45.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.598 ± 0.014

1.653 ± 0.007

5.675 ± 0.011

6.695 ± 0.017

3.748 ± 0.01

5.86 ± 0.018

2.336 ± 0.007

4.264 ± 0.011

5.218 ± 0.018

9.389 ± 0.021

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.47 ± 0.007

3.678 ± 0.008

4.669 ± 0.015

4.262 ± 0.01

6.32 ± 0.016

8.354 ± 0.019

6.003 ± 0.014

7.006 ± 0.013

1.202 ± 0.005

2.6 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski