Thalassorhabdomicrobium marinisediminis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Thalassorhabdomicrobium

Average proteome isoelectric point is 6.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3212 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2T7FYR4|A0A2T7FYR4_9RHOB NAD(+) diphosphatase OS=Thalassorhabdomicrobium marinisediminis OX=2170577 GN=DC363_05380 PE=3 SV=1
MM1 pKa = 7.03MRR3 pKa = 11.84LAAAVICCGQMAAAGGLMDD22 pKa = 3.61RR23 pKa = 11.84TVTFGALAYY32 pKa = 9.71DD33 pKa = 3.8QADD36 pKa = 3.44APIFVGDD43 pKa = 3.59RR44 pKa = 11.84HH45 pKa = 6.05PAVVSDD51 pKa = 4.38RR52 pKa = 11.84IEE54 pKa = 4.03YY55 pKa = 10.35GLRR58 pKa = 11.84PEE60 pKa = 4.86GAQNGWDD67 pKa = 3.51IVPAIIDD74 pKa = 3.58IRR76 pKa = 11.84DD77 pKa = 3.3QQIIVTYY84 pKa = 8.89PDD86 pKa = 3.32TVGGQFPAPAFNGYY100 pKa = 9.4VLDD103 pKa = 4.81FLTEE107 pKa = 4.07CVLFNGAGQDD117 pKa = 3.6QDD119 pKa = 3.43NSTVTLADD127 pKa = 3.3DD128 pKa = 4.85AIFVEE133 pKa = 5.39GSRR136 pKa = 11.84LYY138 pKa = 11.65VNMAGLDD145 pKa = 3.77FGPQTFVVVDD155 pKa = 3.76VDD157 pKa = 4.5VADD160 pKa = 4.78CPLSS164 pKa = 3.55

Molecular weight: 17.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2T7FWE7|A0A2T7FWE7_9RHOB Nicotinate phosphoribosyltransferase OS=Thalassorhabdomicrobium marinisediminis OX=2170577 GN=DC363_09940 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNRR10 pKa = 11.84VRR12 pKa = 11.84KK13 pKa = 8.99NRR15 pKa = 11.84HH16 pKa = 3.77GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.35AGRR28 pKa = 11.84NIINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.97GRR39 pKa = 11.84KK40 pKa = 8.91SLSAA44 pKa = 3.86

Molecular weight: 5.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3212

0

3212

1015165

28

1738

316.1

34.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.359 ± 0.063

0.876 ± 0.013

6.411 ± 0.039

5.95 ± 0.043

3.652 ± 0.03

8.486 ± 0.044

2.058 ± 0.02

5.088 ± 0.033

3.085 ± 0.036

9.944 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.714 ± 0.023

2.543 ± 0.022

5.084 ± 0.033

3.322 ± 0.025

6.763 ± 0.044

4.963 ± 0.028

5.777 ± 0.033

7.39 ± 0.029

1.34 ± 0.019

2.194 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski