Sweetwater Branch virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Alpharhabdovirinae; Tibrovirus; Sweetwater Branch tibrovirus

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0D3R1K9|A0A0D3R1K9_9RHAB Matrix OS=Sweetwater Branch virus OX=1272958 PE=4 SV=1
MM1 pKa = 6.6QRR3 pKa = 11.84QRR5 pKa = 11.84ISIPYY10 pKa = 7.75VTDD13 pKa = 4.16SILTDD18 pKa = 3.32ASKK21 pKa = 11.0AHH23 pKa = 7.05DD24 pKa = 4.31PNEE27 pKa = 4.17VQDD30 pKa = 4.12NEE32 pKa = 4.75SIPTIKK38 pKa = 10.14ILPGLSGDD46 pKa = 3.52QKK48 pKa = 11.42LNEE51 pKa = 4.34LLDD54 pKa = 3.79SRR56 pKa = 11.84EE57 pKa = 4.02LNFRR61 pKa = 11.84TRR63 pKa = 11.84ASSTSSYY70 pKa = 11.54DD71 pKa = 3.41DD72 pKa = 5.05DD73 pKa = 5.03DD74 pKa = 3.92WAEE77 pKa = 4.19SIIDD81 pKa = 3.92LSKK84 pKa = 10.68KK85 pKa = 10.58DD86 pKa = 3.55EE87 pKa = 4.54STTHH91 pKa = 6.69NYY93 pKa = 9.23QKK95 pKa = 11.09GGDD98 pKa = 3.56NSYY101 pKa = 10.91KK102 pKa = 10.9SKK104 pKa = 9.98DD105 pKa = 3.24TSADD109 pKa = 3.36LDD111 pKa = 3.88TPPNKK116 pKa = 9.53KK117 pKa = 10.24DD118 pKa = 3.47SASCTTNDD126 pKa = 3.68ASNLQSIQEE135 pKa = 4.14ANVFDD140 pKa = 4.22TSHH143 pKa = 7.43LYY145 pKa = 10.11PLMEE149 pKa = 4.86LPHH152 pKa = 6.53SFSHH156 pKa = 7.21CIPKK160 pKa = 10.08LQFFLKK166 pKa = 10.49YY167 pKa = 9.14YY168 pKa = 10.73NLYY171 pKa = 10.43EE172 pKa = 4.41DD173 pKa = 3.24VDD175 pKa = 4.13YY176 pKa = 11.69VIDD179 pKa = 4.09KK180 pKa = 11.18DD181 pKa = 3.48NTKK184 pKa = 10.97YY185 pKa = 11.06YY186 pKa = 10.4FFPTKK191 pKa = 10.04KK192 pKa = 8.86WLQGPEE198 pKa = 4.01EE199 pKa = 4.55YY200 pKa = 10.0EE201 pKa = 4.4VRR203 pKa = 11.84TSDD206 pKa = 4.47HH207 pKa = 7.0PIEE210 pKa = 5.41SEE212 pKa = 4.58DD213 pKa = 3.32VTEE216 pKa = 4.12EE217 pKa = 3.85HH218 pKa = 6.95GEE220 pKa = 4.06VHH222 pKa = 6.85ALLDD226 pKa = 3.92FLSKK230 pKa = 10.77GFYY233 pKa = 9.38VEE235 pKa = 3.91KK236 pKa = 10.74RR237 pKa = 11.84NIKK240 pKa = 9.37GKK242 pKa = 11.02YY243 pKa = 9.39YY244 pKa = 10.71FDD246 pKa = 5.17LNNPSLNVNKK256 pKa = 9.93IAKK259 pKa = 9.37QDD261 pKa = 3.51GDD263 pKa = 3.46AHH265 pKa = 6.03GWSDD269 pKa = 3.72SKK271 pKa = 11.35KK272 pKa = 9.42IDD274 pKa = 4.54AIFKK278 pKa = 10.39ASGIYY283 pKa = 9.79RR284 pKa = 11.84AMRR287 pKa = 11.84LKK289 pKa = 10.87AKK291 pKa = 8.75WW292 pKa = 3.07

Molecular weight: 33.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D3R1P9|A0A0D3R1P9_9RHAB Uncharacterized protein OS=Sweetwater Branch virus OX=1272958 PE=4 SV=1
MM1 pKa = 7.66EE2 pKa = 5.43LPKK5 pKa = 10.73SFNLQIDD12 pKa = 4.02YY13 pKa = 11.43NNIWNQISSRR23 pKa = 11.84ISPIWHH29 pKa = 6.39QIKK32 pKa = 9.54KK33 pKa = 8.71WSIIVFILFLTICIVKK49 pKa = 9.59IGKK52 pKa = 9.7FIFQCIKK59 pKa = 9.6IFKK62 pKa = 8.64WLANSVFNYY71 pKa = 9.45MRR73 pKa = 11.84ICFKK77 pKa = 10.75QLSKK81 pKa = 10.71KK82 pKa = 9.86IKK84 pKa = 9.78PKK86 pKa = 10.32QSPQKK91 pKa = 9.4TIYY94 pKa = 9.78KK95 pKa = 9.99DD96 pKa = 3.14RR97 pKa = 11.84SKK99 pKa = 11.29EE100 pKa = 4.04VILDD104 pKa = 3.55VV105 pKa = 4.34

Molecular weight: 12.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

0

8

4187

105

2120

523.4

60.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.559 ± 0.416

2.054 ± 0.275

6.472 ± 0.67

5.35 ± 0.251

4.227 ± 0.212

5.063 ± 0.321

2.747 ± 0.271

8.073 ± 0.634

7.523 ± 0.675

10.103 ± 1.012

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.173 ± 0.303

6.281 ± 0.259

3.845 ± 0.191

3.129 ± 0.291

3.989 ± 0.382

8.574 ± 0.449

5.445 ± 0.32

5.087 ± 0.271

1.791 ± 0.114

4.514 ± 0.466
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski