Candidatus Tremblaya phenacola

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Betaproteobacteria incertae sedis; Candidatus Tremblaya

Average proteome isoelectric point is 8.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 194 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2G0V6U6|A0A2G0V6U6_9PROT 30S ribosomal protein S11 OS=Candidatus Tremblaya phenacola OX=1010676 GN=rpsK PE=3 SV=1
MM1 pKa = 7.37YY2 pKa = 8.12EE3 pKa = 3.89ANEE6 pKa = 4.16ANEE9 pKa = 4.16TMNRR13 pKa = 11.84LTAEE17 pKa = 4.14HH18 pKa = 5.84TTAFHH23 pKa = 6.99PNLLRR28 pKa = 11.84DD29 pKa = 4.06TFPTEE34 pKa = 3.84SSHH37 pKa = 6.78TYY39 pKa = 10.56SLTNILFCLLLTLTPLPTIQNHH61 pKa = 5.87LLIIPEE67 pKa = 4.27INPEE71 pKa = 3.94HH72 pKa = 7.15PYY74 pKa = 10.63VLPRR78 pKa = 11.84YY79 pKa = 8.47CLRR82 pKa = 11.84VMMSNKK88 pKa = 9.7CYY90 pKa = 10.51CLIDD94 pKa = 3.53IDD96 pKa = 5.17SPLEE100 pKa = 3.82PEE102 pKa = 4.2RR103 pKa = 11.84AA104 pKa = 3.49

Molecular weight: 12.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2G0V6X8|A0A2G0V6X8_9PROT IGP synthase cyclase subunit OS=Candidatus Tremblaya phenacola OX=1010676 GN=hisF PE=3 SV=1
MM1 pKa = 7.71LIKK4 pKa = 10.66NSPYY8 pKa = 10.43PMLLRR13 pKa = 11.84TEE15 pKa = 4.45RR16 pKa = 11.84KK17 pKa = 9.24SSGRR21 pKa = 11.84NNIGTITVRR30 pKa = 11.84HH31 pKa = 6.33RR32 pKa = 11.84GCGHH36 pKa = 5.83KK37 pKa = 10.09RR38 pKa = 11.84LYY40 pKa = 9.5RR41 pKa = 11.84TIDD44 pKa = 3.77FYY46 pKa = 11.68RR47 pKa = 11.84NKK49 pKa = 10.65DD50 pKa = 3.65YY51 pKa = 11.18ILGKK55 pKa = 8.89VEE57 pKa = 4.02KK58 pKa = 10.72NEE60 pKa = 3.79YY61 pKa = 10.38DD62 pKa = 3.56PNRR65 pKa = 11.84SANIVLVLYY74 pKa = 10.83ADD76 pKa = 3.82GEE78 pKa = 4.19RR79 pKa = 11.84RR80 pKa = 11.84YY81 pKa = 10.23IIAPNRR87 pKa = 11.84LPIGSVIVSGRR98 pKa = 11.84SVPSQIGNSLSVGLIYY114 pKa = 10.77SGTNVHH120 pKa = 6.82CIEE123 pKa = 4.55LVPANGAKK131 pKa = 10.56LSRR134 pKa = 11.84AAGSYY139 pKa = 8.86ATIISNASHH148 pKa = 5.29YY149 pKa = 8.16TQVRR153 pKa = 11.84LMSGEE158 pKa = 3.77VRR160 pKa = 11.84LINKK164 pKa = 7.52EE165 pKa = 3.67CRR167 pKa = 11.84ATVGIVGNIKK177 pKa = 10.41HH178 pKa = 6.01SLSRR182 pKa = 11.84LDD184 pKa = 3.41KK185 pKa = 10.01AGKK188 pKa = 9.76RR189 pKa = 11.84RR190 pKa = 11.84WLGIRR195 pKa = 11.84PTVRR199 pKa = 11.84GVAMNPIDD207 pKa = 4.06HH208 pKa = 6.89PHH210 pKa = 6.59GGGEE214 pKa = 4.38GKK216 pKa = 6.94TTSGRR221 pKa = 11.84HH222 pKa = 5.12PVSPWGTPTKK232 pKa = 10.67GFKK235 pKa = 8.99TRR237 pKa = 11.84NNKK240 pKa = 9.51QSDD243 pKa = 3.96SYY245 pKa = 11.06IINKK249 pKa = 9.02RR250 pKa = 11.84RR251 pKa = 3.17

Molecular weight: 27.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

194

0

194

60884

46

1351

313.8

35.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.139 ± 0.131

1.703 ± 0.06

3.64 ± 0.087

4.74 ± 0.136

3.446 ± 0.094

5.775 ± 0.118

2.247 ± 0.074

9.668 ± 0.214

8.033 ± 0.141

10.592 ± 0.165

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.088 ± 0.057

5.921 ± 0.116

3.232 ± 0.076

2.505 ± 0.086

5.092 ± 0.101

8.546 ± 0.137

5.709 ± 0.11

6.747 ± 0.167

0.828 ± 0.062

4.349 ± 0.141
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski