Pseudonocardia cypriaca

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Pseudonocardiales; Pseudonocardiaceae; Pseudonocardia

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7704 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A543FPX7|A0A543FPX7_9PSEU Uncharacterized protein DUF1206 OS=Pseudonocardia cypriaca OX=882449 GN=FB388_7347 PE=4 SV=1
MM1 pKa = 7.83AEE3 pKa = 4.32TLSLMEE9 pKa = 4.65FLQALLADD17 pKa = 4.86DD18 pKa = 4.62GLRR21 pKa = 11.84GAFGDD26 pKa = 4.42DD27 pKa = 3.47PQGTLTAHH35 pKa = 7.08GLADD39 pKa = 4.15LTPADD44 pKa = 3.59VHH46 pKa = 6.6DD47 pKa = 4.98ALVLVQDD54 pKa = 4.23TQTVDD59 pKa = 3.62YY60 pKa = 10.15YY61 pKa = 11.77SLDD64 pKa = 3.53AATAPPPPPPTGDD77 pKa = 3.01GHH79 pKa = 5.83EE80 pKa = 4.22AAVEE84 pKa = 3.76YY85 pKa = 10.16LARR88 pKa = 11.84YY89 pKa = 8.18LAGPHH94 pKa = 5.36QVDD97 pKa = 3.95PAEE100 pKa = 4.69GWAEE104 pKa = 3.73PDD106 pKa = 3.28VSSDD110 pKa = 3.16HH111 pKa = 6.37VLLGGFGAGDD121 pKa = 3.51DD122 pKa = 3.69TGYY125 pKa = 11.27GAGSDD130 pKa = 3.73LAADD134 pKa = 4.24DD135 pKa = 4.85GAWTALPPGPAPGHH149 pKa = 6.17ALDD152 pKa = 5.25DD153 pKa = 4.39RR154 pKa = 11.84SILDD158 pKa = 3.76LADD161 pKa = 4.62FGHH164 pKa = 7.26LEE166 pKa = 4.3DD167 pKa = 5.17FGEE170 pKa = 4.71FTAFTDD176 pKa = 5.08LPDD179 pKa = 4.66DD180 pKa = 4.1VPDD183 pKa = 3.85GHH185 pKa = 7.32DD186 pKa = 4.25AGPPLPEE193 pKa = 4.3GAA195 pKa = 4.53

Molecular weight: 20.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A543FQF0|A0A543FQF0_9PSEU Putative F420-dependent oxidoreductase OS=Pseudonocardia cypriaca OX=882449 GN=FB388_7513 PE=4 SV=1
MM1 pKa = 7.53SKK3 pKa = 10.53GKK5 pKa = 8.66RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84THH19 pKa = 5.69GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AIVAARR37 pKa = 11.84RR38 pKa = 11.84GKK40 pKa = 10.12GRR42 pKa = 11.84QRR44 pKa = 11.84LSAA47 pKa = 4.04

Molecular weight: 5.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7704

0

7704

2498483

29

19925

324.3

34.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.376 ± 0.053

0.722 ± 0.009

6.028 ± 0.025

5.526 ± 0.027

2.771 ± 0.021

9.477 ± 0.03

2.19 ± 0.015

3.255 ± 0.02

1.362 ± 0.018

10.677 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.691 ± 0.013

1.541 ± 0.014

6.242 ± 0.028

2.557 ± 0.015

8.366 ± 0.031

4.676 ± 0.025

5.715 ± 0.018

9.514 ± 0.034

1.505 ± 0.013

1.81 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski