Encephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Fungi incertae sedis; Microsporidia; Apansporoblastina; Unikaryonidae; Encephalitozoon; Encephalitozoon cuniculi

Average proteome isoelectric point is 6.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2041 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I7L8P7|I7L8P7_ENCCU Type 1 phosphatases regulator OS=Encephalitozoon cuniculi (strain GB-M1) OX=284813 GN=ECU05_0185 PE=3 SV=1
MM1 pKa = 7.78RR2 pKa = 11.84SINTLIRR9 pKa = 11.84VMLLSEE15 pKa = 4.06ALIYY19 pKa = 10.91ASTEE23 pKa = 3.84GSKK26 pKa = 10.91EE27 pKa = 4.01EE28 pKa = 4.02NSPSITSQQQDD39 pKa = 3.12TSGGEE44 pKa = 3.99EE45 pKa = 3.88EE46 pKa = 4.22NEE48 pKa = 3.91EE49 pKa = 4.07RR50 pKa = 11.84RR51 pKa = 11.84GSGGGEE57 pKa = 3.92EE58 pKa = 4.78APVTAQPQGASGGEE72 pKa = 3.99EE73 pKa = 3.86EE74 pKa = 4.88ADD76 pKa = 3.27GGRR79 pKa = 11.84VVVEE83 pKa = 4.0YY84 pKa = 10.9CSTRR88 pKa = 11.84TQNEE92 pKa = 3.93SGEE95 pKa = 4.53TVEE98 pKa = 4.77TRR100 pKa = 11.84CGCAGDD106 pKa = 5.19CDD108 pKa = 4.07NCCPEE113 pKa = 4.41GMISCLHH120 pKa = 6.0SLGLLL125 pKa = 3.69

Molecular weight: 13.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q8SSA5|Q8SSA5_ENCCU UBIQUITIN CARBOXY-TERMINAL HYDROLASE 14 OS=Encephalitozoon cuniculi (strain GB-M1) OX=284813 GN=ECU03_0660 PE=4 SV=1
MM1 pKa = 7.57GEE3 pKa = 3.81NRR5 pKa = 11.84SRR7 pKa = 11.84RR8 pKa = 11.84TLYY11 pKa = 9.86IRR13 pKa = 11.84NLNRR17 pKa = 11.84RR18 pKa = 11.84LPIRR22 pKa = 11.84EE23 pKa = 3.95VKK25 pKa = 10.1RR26 pKa = 11.84RR27 pKa = 11.84LGLLVSRR34 pKa = 11.84FSGISKK40 pKa = 9.63IKK42 pKa = 9.08MSNRR46 pKa = 11.84PGLLGQAFVHH56 pKa = 6.71LDD58 pKa = 3.53GEE60 pKa = 4.52ITSEE64 pKa = 4.31ILDD67 pKa = 3.71NLEE70 pKa = 3.63GRR72 pKa = 11.84FFLGNVISACPAHH85 pKa = 7.34EE86 pKa = 4.45DD87 pKa = 3.25MCIGRR92 pKa = 11.84RR93 pKa = 11.84RR94 pKa = 11.84STSTRR99 pKa = 11.84TLLVTDD105 pKa = 4.5IPPSVTRR112 pKa = 11.84EE113 pKa = 3.82EE114 pKa = 4.4LEE116 pKa = 3.94GLFRR120 pKa = 11.84GFGGLEE126 pKa = 4.04GIRR129 pKa = 11.84FIRR132 pKa = 11.84VKK134 pKa = 10.81NLAFVDD140 pKa = 4.57FLSSRR145 pKa = 11.84EE146 pKa = 3.71ASAAYY151 pKa = 10.19SHH153 pKa = 7.13FGDD156 pKa = 3.9GLIRR160 pKa = 11.84CGAGEE165 pKa = 4.15MRR167 pKa = 11.84IMPSAQQ173 pKa = 2.84

Molecular weight: 19.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2041

0

2041

694360

25

3436

340.2

38.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.016 ± 0.04

2.024 ± 0.025

5.55 ± 0.033

8.18 ± 0.064

4.796 ± 0.044

6.491 ± 0.059

1.899 ± 0.02

6.734 ± 0.045

7.157 ± 0.052

9.502 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.975 ± 0.024

3.942 ± 0.032

3.269 ± 0.034

2.267 ± 0.03

6.674 ± 0.045

7.966 ± 0.044

4.07 ± 0.033

7.073 ± 0.037

0.754 ± 0.014

3.662 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski