Drosophila pseudoobscura pseudoobscura (Fruit fly)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Eremoneura; Cyclorrhapha; Schizophora;

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 23072 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6I8VZZ7|A0A6I8VZZ7_DROPS Isoform of A0A6I8VYL8 zinc finger protein 497 isoform X6 OS=Drosophila pseudoobscura pseudoobscura OX=46245 GN=LOC6902800 PE=4 SV=1
MM1 pKa = 7.16EE2 pKa = 5.08QIFLAKK8 pKa = 9.59EE9 pKa = 3.79NPAIAEE15 pKa = 3.84IWLDD19 pKa = 3.67NQSGRR24 pKa = 11.84TTFTQTILNDD34 pKa = 3.56LGDD37 pKa = 3.91LVEE40 pKa = 4.3TLEE43 pKa = 4.42TEE45 pKa = 4.43CCISIYY51 pKa = 11.05NVFCDD56 pKa = 4.41NFDD59 pKa = 3.96RR60 pKa = 11.84DD61 pKa = 3.71NCSKK65 pKa = 10.93QDD67 pKa = 3.16IDD69 pKa = 3.64NTGTEE74 pKa = 3.83QCEE77 pKa = 4.34YY78 pKa = 11.22KK79 pKa = 10.43LDD81 pKa = 3.69SHH83 pKa = 6.47MALPNLDD90 pKa = 5.47DD91 pKa = 4.14IDD93 pKa = 3.93IPFF96 pKa = 4.25

Molecular weight: 10.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6I8UQM6|A0A6I8UQM6_DROPS uncharacterized protein LOC4802323 OS=Drosophila pseudoobscura pseudoobscura OX=46245 GN=LOC4802323 PE=4 SV=1
MM1 pKa = 7.62RR2 pKa = 11.84TGQLAVGLLAGSLCIWLTNAATSTTATTSSGTSTTVAATTTTSAATTTTTTAATTAATTSAATTAASTGTVHH74 pKa = 7.17RR75 pKa = 11.84KK76 pKa = 9.32KK77 pKa = 10.47IRR79 pKa = 11.84ISNLKK84 pKa = 8.34WSSKK88 pKa = 9.69RR89 pKa = 11.84RR90 pKa = 11.84IKK92 pKa = 9.26VHH94 pKa = 6.75RR95 pKa = 11.84GTTNRR100 pKa = 11.84STRR103 pKa = 11.84SRR105 pKa = 11.84SVLGRR110 pKa = 11.84RR111 pKa = 11.84ILRR114 pKa = 11.84SRR116 pKa = 11.84NGSRR120 pKa = 11.84ARR122 pKa = 11.84VLVLNQRR129 pKa = 11.84RR130 pKa = 11.84AQGG133 pKa = 3.17

Molecular weight: 13.93 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

14407

8665

23072

17929357

31

22987

777.1

86.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.698 ± 0.023

1.758 ± 0.031

5.297 ± 0.021

6.645 ± 0.041

3.153 ± 0.014

6.184 ± 0.028

2.669 ± 0.011

4.716 ± 0.016

5.487 ± 0.034

8.651 ± 0.026

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.238 ± 0.01

4.517 ± 0.017

5.854 ± 0.034

5.614 ± 0.026

5.459 ± 0.017

8.654 ± 0.029

5.973 ± 0.019

5.78 ± 0.02

0.903 ± 0.006

2.752 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski