HIV-1 M_02CD.MBTB047

Taxonomy: Viruses; Riboviria; Pararnavirae; Artverviricota; Revtraviricetes; Ortervirales; Retroviridae; Orthoretrovirinae; Lentivirus; Human immunodeficiency virus 1; HIV-1 group M; HIV-1 group M unknown subgroup

Average proteome isoelectric point is 6.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C7TQ97|C7TQ97_9HIV1 Envelope glycoprotein gp160 OS=HIV-1 M_02CD.MBTB047 OX=576902 GN=gp160 PE=3 SV=1
MM1 pKa = 7.54TPLQIYY7 pKa = 9.84AIVGLIVALILAIVVWSIVLVEE29 pKa = 3.92YY30 pKa = 10.82KK31 pKa = 10.56KK32 pKa = 10.39ILKK35 pKa = 8.62QRR37 pKa = 11.84KK38 pKa = 8.07IDD40 pKa = 3.68RR41 pKa = 11.84LLDD44 pKa = 4.54RR45 pKa = 11.84IRR47 pKa = 11.84DD48 pKa = 3.67RR49 pKa = 11.84AEE51 pKa = 4.09DD52 pKa = 3.62SGNEE56 pKa = 3.97SDD58 pKa = 5.62GDD60 pKa = 4.05TEE62 pKa = 4.08EE63 pKa = 5.17LSALVEE69 pKa = 4.39MGDD72 pKa = 3.84HH73 pKa = 6.47NPWGYY78 pKa = 11.04NVLL81 pKa = 3.79

Molecular weight: 9.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C7TQ95|C7TQ95_9HIV1 Protein Vpr OS=HIV-1 M_02CD.MBTB047 OX=576902 GN=vpr PE=3 SV=1
MM1 pKa = 7.3EE2 pKa = 5.21NRR4 pKa = 11.84WQVMIVWQVDD14 pKa = 3.04RR15 pKa = 11.84MRR17 pKa = 11.84IRR19 pKa = 11.84TWHH22 pKa = 6.19SLVKK26 pKa = 9.46YY27 pKa = 10.38HH28 pKa = 6.79MYY30 pKa = 10.19RR31 pKa = 11.84SKK33 pKa = 10.72KK34 pKa = 9.53AKK36 pKa = 8.07GWLYY40 pKa = 8.6RR41 pKa = 11.84HH42 pKa = 6.62HH43 pKa = 7.0YY44 pKa = 9.16EE45 pKa = 4.05SRR47 pKa = 11.84HH48 pKa = 5.45PKK50 pKa = 9.69VSSEE54 pKa = 3.72VHH56 pKa = 5.93IPLGDD61 pKa = 3.34ARR63 pKa = 11.84LVIRR67 pKa = 11.84TYY69 pKa = 10.12WGLQTGEE76 pKa = 4.5RR77 pKa = 11.84EE78 pKa = 3.69WHH80 pKa = 6.42LGHH83 pKa = 6.45GVSIEE88 pKa = 3.6WRR90 pKa = 11.84LRR92 pKa = 11.84RR93 pKa = 11.84YY94 pKa = 7.75STQVDD99 pKa = 3.64PGLADD104 pKa = 3.49QLIHH108 pKa = 6.35LQYY111 pKa = 10.56FEE113 pKa = 5.17CFSDD117 pKa = 3.53SAIRR121 pKa = 11.84KK122 pKa = 9.26AIVGQIIIHH131 pKa = 5.56KK132 pKa = 9.88CEE134 pKa = 3.81YY135 pKa = 9.48QAGHH139 pKa = 6.48KK140 pKa = 10.18KK141 pKa = 10.44VGSLQYY147 pKa = 10.6LALKK151 pKa = 10.56ALVATKK157 pKa = 9.14RR158 pKa = 11.84TKK160 pKa = 10.56PPLPSVKK167 pKa = 10.27KK168 pKa = 9.35LTEE171 pKa = 4.22DD172 pKa = 3.5RR173 pKa = 11.84WNKK176 pKa = 7.33PQKK179 pKa = 9.26TKK181 pKa = 8.88GHH183 pKa = 6.42RR184 pKa = 11.84EE185 pKa = 3.74SHH187 pKa = 5.49TMNGHH192 pKa = 5.96

Molecular weight: 22.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

2864

81

1430

477.3

54.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.564 ± 0.358

1.781 ± 0.416

4.469 ± 0.344

6.564 ± 0.806

2.758 ± 0.392

7.577 ± 0.481

2.304 ± 0.651

6.948 ± 0.816

7.158 ± 1.255

8.415 ± 0.77

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.2 ± 0.15

4.888 ± 0.991

4.504 ± 0.717

5.656 ± 0.652

5.552 ± 0.827

4.888 ± 0.365

5.517 ± 0.443

6.564 ± 0.401

2.933 ± 0.297

2.758 ± 0.311
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski