Psychroflexus halocasei

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Psychroflexus

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2462 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H3VQ64|A0A1H3VQ64_9FLAO Pimeloyl-ACP methyl ester carboxylesterase OS=Psychroflexus halocasei OX=908615 GN=SAMN05421540_101203 PE=4 SV=1
MM1 pKa = 7.54KK2 pKa = 10.43NYY4 pKa = 10.2LKK6 pKa = 10.93YY7 pKa = 10.5IGISACAMFLSCEE20 pKa = 4.23PEE22 pKa = 3.63FDD24 pKa = 4.03NQVQEE29 pKa = 3.66NDD31 pKa = 3.34IYY33 pKa = 11.68VSGDD37 pKa = 2.9ADD39 pKa = 3.43FTNFVSVGNSLTAGYY54 pKa = 10.34ADD56 pKa = 3.22NALYY60 pKa = 10.7VQGQEE65 pKa = 3.99SSFPNIMATKK75 pKa = 9.93FEE77 pKa = 4.42LVGGGEE83 pKa = 4.73FKK85 pKa = 10.97QPMMADD91 pKa = 3.21NLGGLLLGGQQISEE105 pKa = 4.07NRR107 pKa = 11.84LVLSVTSGSPAPAILAGTPQTEE129 pKa = 4.26ITNKK133 pKa = 10.39LSGSFNNMGVPGAKK147 pKa = 9.46SFHH150 pKa = 6.36LAAPGYY156 pKa = 9.47GNPAGITSGQANPYY170 pKa = 8.17FARR173 pKa = 11.84FASSEE178 pKa = 4.23SATVIEE184 pKa = 5.37DD185 pKa = 3.55AASQNPTFFSLWIGNNDD202 pKa = 2.98ILSFATSGGIGVDD215 pKa = 3.19QAGNLDD221 pKa = 3.65PSTYY225 pKa = 10.37GSNDD229 pKa = 2.61ITDD232 pKa = 4.16PNVFASVYY240 pKa = 10.66NDD242 pKa = 4.04LLNALNANASGGVVFNIPDD261 pKa = 3.33VTNIPFFTTVPNNALALDD279 pKa = 4.44AAQAQGLTGFFQAFAGIATQTLIQQGVPAAQAQALAAQYY318 pKa = 10.87AITFNEE324 pKa = 4.43GPNRR328 pKa = 11.84FLISVEE334 pKa = 4.14PSQQNPFGFRR344 pKa = 11.84QMTDD348 pKa = 2.95EE349 pKa = 4.54EE350 pKa = 4.96LLLLTIDD357 pKa = 4.86RR358 pKa = 11.84GALQNSGYY366 pKa = 10.71GSVALTPEE374 pKa = 4.14VMQVLGILQQGGTPTPQQAMLVLDD398 pKa = 4.21AVNPIEE404 pKa = 6.27DD405 pKa = 4.34EE406 pKa = 4.37DD407 pKa = 5.13ALDD410 pKa = 4.41DD411 pKa = 4.41DD412 pKa = 5.67EE413 pKa = 6.9IMMVQTAKK421 pKa = 10.72EE422 pKa = 4.27SFNATIEE429 pKa = 4.12GLAQANGLAFVDD441 pKa = 3.54VDD443 pKa = 3.75EE444 pKa = 4.89LMKK447 pKa = 10.66EE448 pKa = 4.22VSTTGIVYY456 pKa = 10.06EE457 pKa = 4.31SGVMTSAFVTGGAFSLDD474 pKa = 3.55GVHH477 pKa = 6.15LTPRR481 pKa = 11.84GYY483 pKa = 11.4AFIASEE489 pKa = 5.06AIQSINEE496 pKa = 4.5TYY498 pKa = 10.71GSTVPTVNIGNFGTVTFSDD517 pKa = 4.3DD518 pKa = 3.46VQQ520 pKa = 4.37

Molecular weight: 54.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H3XJQ8|A0A1H3XJQ8_9FLAO Putative fluoride ion transporter CrcB OS=Psychroflexus halocasei OX=908615 GN=crcB PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.59RR3 pKa = 11.84TFQPSKK9 pKa = 10.15RR10 pKa = 11.84KK11 pKa = 9.6RR12 pKa = 11.84KK13 pKa = 8.29NKK15 pKa = 9.34HH16 pKa = 4.03GFRR19 pKa = 11.84EE20 pKa = 4.09RR21 pKa = 11.84MASKK25 pKa = 10.5NGRR28 pKa = 11.84KK29 pKa = 9.21VLSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.84GRR39 pKa = 11.84KK40 pKa = 7.91RR41 pKa = 11.84LSVSSEE47 pKa = 3.0LSMRR51 pKa = 11.84KK52 pKa = 8.92RR53 pKa = 3.45

Molecular weight: 6.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2462

0

2462

806779

29

3454

327.7

37.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.062 ± 0.038

0.668 ± 0.014

5.975 ± 0.043

7.057 ± 0.047

5.339 ± 0.04

5.878 ± 0.043

1.881 ± 0.021

8.084 ± 0.046

8.351 ± 0.067

9.328 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.218 ± 0.024

6.003 ± 0.052

3.153 ± 0.024

3.829 ± 0.032

3.366 ± 0.032

6.695 ± 0.039

5.207 ± 0.044

5.863 ± 0.039

0.936 ± 0.016

4.105 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski