Propionibacterium phage B3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Anatolevirus; Propionibacterium virus B3

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 57 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1D8ETC6|A0A1D8ETC6_9CAUD Uncharacterized protein OS=Propionibacterium phage B3 OX=1897533 GN=7 PE=4 SV=1
MM1 pKa = 7.24RR2 pKa = 11.84HH3 pKa = 6.3KK4 pKa = 10.45IKK6 pKa = 9.95STEE9 pKa = 3.93TRR11 pKa = 11.84IKK13 pKa = 10.53AGPDD17 pKa = 2.83AGLKK21 pKa = 10.55DD22 pKa = 4.23GEE24 pKa = 4.36FTAYY28 pKa = 9.71PSTFTRR34 pKa = 11.84QPDD37 pKa = 3.55SYY39 pKa = 11.08GDD41 pKa = 3.79VVAKK45 pKa = 10.44GAFADD50 pKa = 4.94TIKK53 pKa = 10.47AWKK56 pKa = 10.17DD57 pKa = 3.15SGRR60 pKa = 11.84TMPGLFGHH68 pKa = 6.96RR69 pKa = 11.84MDD71 pKa = 5.36DD72 pKa = 3.43PEE74 pKa = 5.47LFVASATDD82 pKa = 3.38MGEE85 pKa = 4.6DD86 pKa = 3.72DD87 pKa = 4.15HH88 pKa = 7.25GWWVKK93 pKa = 10.98GIFDD97 pKa = 5.34LDD99 pKa = 3.51NPKK102 pKa = 10.38AVQVYY107 pKa = 9.75RR108 pKa = 11.84LVKK111 pKa = 10.17SGRR114 pKa = 11.84LSQLSFAFDD123 pKa = 3.43VDD125 pKa = 4.54DD126 pKa = 4.2EE127 pKa = 4.7ATVEE131 pKa = 4.4LDD133 pKa = 4.77DD134 pKa = 3.93GTKK137 pKa = 10.66ANEE140 pKa = 3.72LRR142 pKa = 11.84KK143 pKa = 10.22LKK145 pKa = 10.6VFEE148 pKa = 4.57FSFVPIGANQDD159 pKa = 3.27TSIVAVKK166 pKa = 9.81TPGDD170 pKa = 3.97DD171 pKa = 3.5QSSDD175 pKa = 3.76DD176 pKa = 4.25GDD178 pKa = 3.83AALSDD183 pKa = 3.54EE184 pKa = 4.37AAAVLSEE191 pKa = 4.15VLDD194 pKa = 3.65VLTNSVEE201 pKa = 4.25KK202 pKa = 10.48IKK204 pKa = 11.14NLLAPSGSGSDD215 pKa = 4.06DD216 pKa = 3.49VQASGQADD224 pKa = 3.76ANDD227 pKa = 4.54EE228 pKa = 4.37EE229 pKa = 5.15PSSAPAKK236 pKa = 9.96SKK238 pKa = 10.15EE239 pKa = 4.17HH240 pKa = 5.84GVSPSAQTLSLQIDD254 pKa = 3.91IAALSGQEE262 pKa = 3.77GDD264 pKa = 3.71SRR266 pKa = 4.07

Molecular weight: 28.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1D8ETK1|A0A1D8ETK1_9CAUD Uncharacterized protein OS=Propionibacterium phage B3 OX=1897533 GN=27 PE=4 SV=1
MM1 pKa = 7.62SGDD4 pKa = 3.74ADD6 pKa = 3.84VTKK9 pKa = 10.29QAPLIRR15 pKa = 11.84RR16 pKa = 11.84VWAMLAEE23 pKa = 4.6PKK25 pKa = 10.38SVTIMMTFAYY35 pKa = 10.01GGLLGMGFSSLAGASPGGLRR55 pKa = 11.84DD56 pKa = 3.48MMGGLLIVGGVCGLIGCPAGQWWIEE81 pKa = 3.97RR82 pKa = 11.84AGLVAVAAAFAGHH95 pKa = 7.24LSFVVAVSPPDD106 pKa = 3.96GPWEE110 pKa = 3.94VASAIGVLVLVATRR124 pKa = 11.84WIRR127 pKa = 11.84IRR129 pKa = 11.84ALPADD134 pKa = 4.1PRR136 pKa = 11.84RR137 pKa = 11.84ARR139 pKa = 11.84PNNAGRR145 pKa = 11.84GRR147 pKa = 11.84HH148 pKa = 5.63DD149 pKa = 3.16

Molecular weight: 15.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

57

0

57

11335

27

1132

198.9

21.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.581 ± 0.606

0.909 ± 0.153

6.458 ± 0.449

4.896 ± 0.372

2.929 ± 0.195

8.928 ± 0.579

1.87 ± 0.236

4.482 ± 0.386

3.917 ± 0.282

7.834 ± 0.368

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.311 ± 0.17

2.267 ± 0.153

5.558 ± 0.363

3.802 ± 0.201

6.917 ± 0.539

6.361 ± 0.35

6.237 ± 0.297

7.737 ± 0.335

2.62 ± 0.301

1.385 ± 0.168
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski