Curtobacterium sp. Leaf261

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Curtobacterium; unclassified Curtobacterium

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3343 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q4UMZ8|A0A0Q4UMZ8_9MICO Alanine dehydrogenase OS=Curtobacterium sp. Leaf261 OX=1736311 GN=ASF23_12690 PE=3 SV=1
MM1 pKa = 7.07STDD4 pKa = 2.96TTLLVTGMTCEE15 pKa = 3.96HH16 pKa = 6.82CVMSVTEE23 pKa = 4.28EE24 pKa = 4.06LSDD27 pKa = 3.7VAGVDD32 pKa = 3.66AVAVDD37 pKa = 4.49LVDD40 pKa = 4.54GGTSTVTVSASTPLDD55 pKa = 4.09DD56 pKa = 5.47DD57 pKa = 4.13ALRR60 pKa = 11.84AAIEE64 pKa = 4.11EE65 pKa = 4.13AGYY68 pKa = 8.77TPVARR73 pKa = 4.73

Molecular weight: 7.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q4VBB7|A0A0Q4VBB7_9MICO DNA helicase OS=Curtobacterium sp. Leaf261 OX=1736311 GN=ASF23_03425 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 9.97KK16 pKa = 9.33HH17 pKa = 4.25GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84SILAARR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.52GRR40 pKa = 11.84TEE42 pKa = 4.14LSAA45 pKa = 4.86

Molecular weight: 5.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3343

0

3343

1097335

29

3663

328.2

34.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.398 ± 0.058

0.448 ± 0.01

6.666 ± 0.043

4.857 ± 0.045

3.029 ± 0.028

9.281 ± 0.043

2.023 ± 0.022

4.159 ± 0.029

1.813 ± 0.035

9.415 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.746 ± 0.015

1.879 ± 0.025

5.403 ± 0.031

2.77 ± 0.026

7.276 ± 0.053

5.95 ± 0.04

6.914 ± 0.068

9.673 ± 0.052

1.463 ± 0.018

1.835 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski