Calla lily chlorotic spot virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Ellioviricetes; Bunyavirales; Tospoviridae; Orthotospovirus; Calla lily chlorotic spot orthotospovirus

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H4WN56|A0A2H4WN56_9VIRU Nucleoprotein OS=Calla lily chlorotic spot virus OX=309542 PE=3 SV=1
MM1 pKa = 7.43KK2 pKa = 10.11KK3 pKa = 10.44YY4 pKa = 10.55YY5 pKa = 10.58LPLYY9 pKa = 9.91CLSLLLLFFVSEE21 pKa = 4.29VFLLSKK27 pKa = 9.69TEE29 pKa = 3.94HH30 pKa = 6.97DD31 pKa = 4.1SEE33 pKa = 4.03ITRR36 pKa = 11.84IRR38 pKa = 11.84DD39 pKa = 3.51RR40 pKa = 11.84YY41 pKa = 9.72PVDD44 pKa = 4.83DD45 pKa = 5.4PDD47 pKa = 5.87DD48 pKa = 3.92LVEE51 pKa = 4.94NYY53 pKa = 10.76GEE55 pKa = 4.46YY56 pKa = 9.98IPHH59 pKa = 6.53RR60 pKa = 11.84TPEE63 pKa = 3.96ADD65 pKa = 3.43KK66 pKa = 8.95PTKK69 pKa = 10.02VLSRR73 pKa = 11.84ILRR76 pKa = 11.84DD77 pKa = 3.46SSSTEE82 pKa = 3.6APRR85 pKa = 11.84LDD87 pKa = 4.97SFDD90 pKa = 3.49CMKK93 pKa = 10.53FEE95 pKa = 5.84KK96 pKa = 9.09KK97 pKa = 9.42QCMIKK102 pKa = 10.61GLSDD106 pKa = 3.94FNAHH110 pKa = 5.15YY111 pKa = 10.71QIDD114 pKa = 3.95NGNEE118 pKa = 3.73IVSCISDD125 pKa = 3.47SPNIFEE131 pKa = 3.85ICQYY135 pKa = 10.55EE136 pKa = 4.44KK137 pKa = 10.93EE138 pKa = 4.22FKK140 pKa = 9.05KK141 pKa = 9.42TKK143 pKa = 9.73FSKK146 pKa = 10.89LPVLPVLKK154 pKa = 10.71LEE156 pKa = 4.26NKK158 pKa = 9.53RR159 pKa = 11.84VLEE162 pKa = 4.22IGSKK166 pKa = 10.49FFFVDD171 pKa = 3.65KK172 pKa = 11.1SNNPVNIDD180 pKa = 4.41PISGLQSATVSKK192 pKa = 10.91LSVRR196 pKa = 11.84LSGDD200 pKa = 3.44CKK202 pKa = 10.37INQIMMSSPYY212 pKa = 9.45QIKK215 pKa = 9.64VQSEE219 pKa = 4.28EE220 pKa = 4.26PIGYY224 pKa = 9.58LIKK227 pKa = 10.67NIKK230 pKa = 9.54DD231 pKa = 3.49SKK233 pKa = 10.41LGEE236 pKa = 4.27IKK238 pKa = 9.83TVTGDD243 pKa = 3.24STINFSPDD251 pKa = 3.22EE252 pKa = 4.05LDD254 pKa = 3.76GNHH257 pKa = 6.63FLLCGDD263 pKa = 4.19RR264 pKa = 11.84SSLITKK270 pKa = 9.82VDD272 pKa = 3.19IPVRR276 pKa = 11.84NCVSKK281 pKa = 11.02FSDD284 pKa = 3.67EE285 pKa = 3.93PKK287 pKa = 10.62KK288 pKa = 10.85IFFCTNFSYY297 pKa = 10.0FKK299 pKa = 9.54WLFVFLVIFFPINWLIWKK317 pKa = 7.91TKK319 pKa = 10.1DD320 pKa = 4.25SLVVWYY326 pKa = 9.19DD327 pKa = 3.09IIGIITYY334 pKa = 9.17PILWIMNRR342 pKa = 11.84SWPYY346 pKa = 9.4FPLRR350 pKa = 11.84CRR352 pKa = 11.84ICGCFSLLTHH362 pKa = 6.65KK363 pKa = 10.75CSEE366 pKa = 4.23KK367 pKa = 10.65CVCNQSKK374 pKa = 8.94TSKK377 pKa = 8.31THH379 pKa = 4.95TSEE382 pKa = 4.37CYY384 pKa = 10.58LFIKK388 pKa = 10.54DD389 pKa = 3.42RR390 pKa = 11.84TEE392 pKa = 3.96WNCMSLIQQFQFTINTKK409 pKa = 9.58ISANFLVFITKK420 pKa = 9.52MIIASILLSYY430 pKa = 10.13LPSSMAAPKK439 pKa = 9.97SICVDD444 pKa = 2.78KK445 pKa = 11.12CYY447 pKa = 10.48FSSDD451 pKa = 3.89LKK453 pKa = 11.69SMTTSRR459 pKa = 11.84SGLSNNQYY467 pKa = 7.49EE468 pKa = 4.52TCDD471 pKa = 3.63CSIGNVITEE480 pKa = 4.58TIYY483 pKa = 10.81QEE485 pKa = 4.58GIPVSRR491 pKa = 11.84ATSVNNCVAGSTACMSEE508 pKa = 4.16GNQAEE513 pKa = 4.18NLFACRR519 pKa = 11.84YY520 pKa = 7.87GCHH523 pKa = 6.27SLVSIKK529 pKa = 10.49NIPEE533 pKa = 4.1TEE535 pKa = 3.98FVPQYY540 pKa = 10.86KK541 pKa = 10.53GISFSGNLTSLKK553 pKa = 9.82IANRR557 pKa = 11.84LRR559 pKa = 11.84NGYY562 pKa = 10.01LDD564 pKa = 4.84DD565 pKa = 4.44SSEE568 pKa = 4.28SKK570 pKa = 10.56ILEE573 pKa = 4.27KK574 pKa = 10.72EE575 pKa = 3.84SSKK578 pKa = 10.72EE579 pKa = 3.62YY580 pKa = 10.87KK581 pKa = 9.9FYY583 pKa = 9.97KK584 pKa = 8.1TLKK587 pKa = 10.57VEE589 pKa = 5.38DD590 pKa = 4.34IPPEE594 pKa = 3.93NLMPRR599 pKa = 11.84QSLVFSSEE607 pKa = 3.33VDD609 pKa = 3.03GKK611 pKa = 9.39YY612 pKa = 10.27RR613 pKa = 11.84YY614 pKa = 9.01MIEE617 pKa = 3.83MDD619 pKa = 3.39IKK621 pKa = 10.95AKK623 pKa = 9.87TGSIYY628 pKa = 10.82LLNDD632 pKa = 3.94DD633 pKa = 4.53STHH636 pKa = 7.34SPMEE640 pKa = 3.25FMIYY644 pKa = 9.46VKK646 pKa = 10.49SVGVEE651 pKa = 3.51YY652 pKa = 10.47DD653 pKa = 3.3VKK655 pKa = 11.11YY656 pKa = 10.55KK657 pKa = 11.09YY658 pKa = 9.57STAKK662 pKa = 9.93IDD664 pKa = 3.79TTISDD669 pKa = 4.15YY670 pKa = 11.45LVTCTGTCADD680 pKa = 4.67CRR682 pKa = 11.84KK683 pKa = 9.69QKK685 pKa = 10.69PKK687 pKa = 10.63VGKK690 pKa = 10.3LDD692 pKa = 3.61FCVVPTSWWGCEE704 pKa = 3.86EE705 pKa = 4.24VGCLAINEE713 pKa = 4.33GAICGHH719 pKa = 5.94CTNVYY724 pKa = 10.35DD725 pKa = 5.55LSSTVNIYY733 pKa = 9.29QVIQSHH739 pKa = 5.04VTAEE743 pKa = 3.95ICIKK747 pKa = 10.83SIDD750 pKa = 3.82GYY752 pKa = 10.32NCKK755 pKa = 9.89KK756 pKa = 10.27HH757 pKa = 6.6SDD759 pKa = 3.51RR760 pKa = 11.84SPIQTDD766 pKa = 3.95HH767 pKa = 6.6YY768 pKa = 9.96QLDD771 pKa = 3.97MTVDD775 pKa = 3.35LHH777 pKa = 7.06NDD779 pKa = 3.14YY780 pKa = 10.9MSTDD784 pKa = 3.08KK785 pKa = 11.27LFAVNKK791 pKa = 6.4QQKK794 pKa = 9.15ILTGSISDD802 pKa = 4.26LGDD805 pKa = 3.6FSSSAFGHH813 pKa = 5.32PQITIDD819 pKa = 3.86GTPLSVPSSLGQNDD833 pKa = 3.99FTWSCSAIGEE843 pKa = 4.23KK844 pKa = 10.16KK845 pKa = 10.45INIRR849 pKa = 11.84QCGLYY854 pKa = 8.99TYY856 pKa = 10.59SAIYY860 pKa = 9.95VLSTSKK866 pKa = 10.61DD867 pKa = 3.53YY868 pKa = 11.7SVMDD872 pKa = 3.65EE873 pKa = 4.57EE874 pKa = 4.97SNKK877 pKa = 10.68LYY879 pKa = 9.94MEE881 pKa = 4.67KK882 pKa = 10.79DD883 pKa = 3.46FLVGKK888 pKa = 9.62LKK890 pKa = 11.0VVVEE894 pKa = 4.34MPKK897 pKa = 10.84EE898 pKa = 4.05MFKK901 pKa = 10.89KK902 pKa = 10.52LPSKK906 pKa = 10.69PMLSEE911 pKa = 3.79TRR913 pKa = 11.84AVCSGCAQCAMGISCNLTYY932 pKa = 10.65TSDD935 pKa = 3.43TTFSARR941 pKa = 11.84LMMDD945 pKa = 3.24SCSFKK950 pKa = 10.49SDD952 pKa = 3.42QIGTVLGPNEE962 pKa = 4.27KK963 pKa = 10.32NIKK966 pKa = 9.66AYY968 pKa = 10.13CSNEE972 pKa = 3.86IQSKK976 pKa = 7.57SLKK979 pKa = 10.26LIPEE983 pKa = 4.57DD984 pKa = 3.8QSEE987 pKa = 4.53LTVDD991 pKa = 3.5IPIDD995 pKa = 3.62EE996 pKa = 4.86FVPADD1001 pKa = 3.67QDD1003 pKa = 4.22TIIHH1007 pKa = 6.6FDD1009 pKa = 3.87DD1010 pKa = 5.37KK1011 pKa = 11.3SAHH1014 pKa = 6.98DD1015 pKa = 4.17EE1016 pKa = 4.3NKK1018 pKa = 9.75HH1019 pKa = 6.53HH1020 pKa = 7.32SDD1022 pKa = 2.98TSMATLWDD1030 pKa = 3.48WVKK1033 pKa = 11.32APFNWVASFFGTFFDD1048 pKa = 3.51MVRR1051 pKa = 11.84IVLVVIAICIGIYY1064 pKa = 9.7ILSYY1068 pKa = 10.12VYY1070 pKa = 10.6KK1071 pKa = 10.77LSRR1074 pKa = 11.84SYY1076 pKa = 11.21YY1077 pKa = 8.44NEE1079 pKa = 3.55KK1080 pKa = 10.1RR1081 pKa = 11.84KK1082 pKa = 10.2HH1083 pKa = 6.48KK1084 pKa = 9.98MEE1086 pKa = 5.2DD1087 pKa = 3.37SISAIEE1093 pKa = 5.05SDD1095 pKa = 4.49LLLNDD1100 pKa = 3.58RR1101 pKa = 11.84TGMISTRR1108 pKa = 11.84KK1109 pKa = 9.65RR1110 pKa = 11.84NPPPKK1115 pKa = 9.94NYY1117 pKa = 9.73QFSLEE1122 pKa = 4.08LL1123 pKa = 4.36

Molecular weight: 127.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H4WNB2|A0A2H4WNB2_9VIRU NSm OS=Calla lily chlorotic spot virus OX=309542 PE=4 SV=1
MM1 pKa = 7.39SNVRR5 pKa = 11.84GLTHH9 pKa = 6.57QKK11 pKa = 9.93IKK13 pKa = 10.81EE14 pKa = 3.91LLAGGKK20 pKa = 9.93ADD22 pKa = 4.36VEE24 pKa = 4.4IEE26 pKa = 4.6SDD28 pKa = 3.54EE29 pKa = 4.0QTQGFNFSTFYY40 pKa = 10.78EE41 pKa = 4.59SNKK44 pKa = 10.24SGPEE48 pKa = 3.74FTFTTGINILKK59 pKa = 9.96CRR61 pKa = 11.84KK62 pKa = 9.08QVFAACKK69 pKa = 7.66SGKK72 pKa = 9.98YY73 pKa = 8.1EE74 pKa = 3.9FCGKK78 pKa = 10.13KK79 pKa = 10.07VVATTDD85 pKa = 3.42EE86 pKa = 4.36VSATDD91 pKa = 3.22WTFKK95 pKa = 9.7RR96 pKa = 11.84TEE98 pKa = 3.61AFIRR102 pKa = 11.84ARR104 pKa = 11.84LISMVEE110 pKa = 4.01HH111 pKa = 6.6TSDD114 pKa = 3.36EE115 pKa = 4.22ATKK118 pKa = 10.19KK119 pKa = 10.24QMYY122 pKa = 8.36IKK124 pKa = 10.74AMEE127 pKa = 4.47LPLVVAYY134 pKa = 9.8GLNVPAEE141 pKa = 4.32FNSAALRR148 pKa = 11.84LMLCIGGPLPLLSSIPGLAPVCFPLAYY175 pKa = 9.5FQNVKK180 pKa = 10.4KK181 pKa = 10.14EE182 pKa = 3.74QLGIKK187 pKa = 10.0NFSTYY192 pKa = 8.49EE193 pKa = 3.97QICKK197 pKa = 8.74VAKK200 pKa = 9.55VLSAASVEE208 pKa = 5.13FIDD211 pKa = 4.22KK212 pKa = 10.31TKK214 pKa = 11.15DD215 pKa = 3.39LFDD218 pKa = 3.8STVKK222 pKa = 10.91LLGDD226 pKa = 4.41SNPGTAGAISLHH238 pKa = 6.63KK239 pKa = 10.8YY240 pKa = 9.96NNQLKK245 pKa = 10.19QMEE248 pKa = 4.39TAFNSKK254 pKa = 8.66LTVDD258 pKa = 5.29DD259 pKa = 5.34FGQNSKK265 pKa = 10.14QVVKK269 pKa = 10.84KK270 pKa = 10.78KK271 pKa = 9.49NTDD274 pKa = 3.26LALL277 pKa = 4.16

Molecular weight: 30.57 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

5052

277

2883

1010.4

115.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.018 ± 0.569

2.415 ± 0.495

5.8 ± 0.286

6.453 ± 0.488

4.889 ± 0.18

4.078 ± 0.366

1.92 ± 0.196

8.036 ± 0.378

8.888 ± 0.317

8.571 ± 0.492

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.662 ± 0.639

6.433 ± 0.643

3.088 ± 0.485

2.831 ± 0.2

3.207 ± 0.135

9.857 ± 0.698

5.562 ± 0.21

5.523 ± 0.363

0.792 ± 0.172

3.979 ± 0.413
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski