Methylophilaceae phage P19250A

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 58 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H4FYM5|A0A2H4FYM5_9CAUD Uncharacterized protein OS=Methylophilaceae phage P19250A OX=1913040 GN=P19250A_0033 PE=4 SV=1
MM1 pKa = 7.29ATLSITPAPANDD13 pKa = 3.02IAVSVNATDD22 pKa = 3.51VTLSQGTTLNVEE34 pKa = 4.38VTPTPATTVVVDD46 pKa = 4.32RR47 pKa = 11.84GVTGASGLSGYY58 pKa = 10.0SGYY61 pKa = 10.67SGYY64 pKa = 10.71SGYY67 pKa = 11.05SGFSGISGASGISGFSGISGYY88 pKa = 10.66SGEE91 pKa = 4.51QGTSINIVGSVATPEE106 pKa = 4.31DD107 pKa = 4.32LPPSGNLNDD116 pKa = 4.2AYY118 pKa = 9.72IVQSNGDD125 pKa = 3.82LYY127 pKa = 11.61VWDD130 pKa = 4.37GSAWINVGQIVGPQGEE146 pKa = 4.52SGYY149 pKa = 10.59SGYY152 pKa = 11.02SGISGFSGNSGISGYY167 pKa = 10.98SGFSGVNGASGFSGEE182 pKa = 4.19SGYY185 pKa = 11.34SGFSGISGFSGEE197 pKa = 4.54SGISGWSGYY206 pKa = 10.76SGINGLSGYY215 pKa = 10.57SGFNGFSGISGWSGEE230 pKa = 4.49SGFSGYY236 pKa = 10.55SGASGISGWSGDD248 pKa = 3.51SGISGFSGFSGDD260 pKa = 3.88SGISGYY266 pKa = 10.98SGFSGISGYY275 pKa = 10.78SGASGISGWSGEE287 pKa = 4.16IGASGYY293 pKa = 10.8SGISGWSGDD302 pKa = 3.51SGISGFSGEE311 pKa = 4.66SGASGISGFSGWSGISGYY329 pKa = 10.73SGISGYY335 pKa = 10.97SGINGLSGYY344 pKa = 10.41SGINGASGEE353 pKa = 4.29SGYY356 pKa = 10.95SGWSGEE362 pKa = 3.96IGASGISGYY371 pKa = 10.44SGYY374 pKa = 11.0SGFNGAIGSSGISGFSGYY392 pKa = 10.12SGEE395 pKa = 4.49IGASGDD401 pKa = 3.57SGFSGWSGEE410 pKa = 4.12VGASGISGFSGYY422 pKa = 10.69SGFSGHH428 pKa = 6.09SGEE431 pKa = 4.68VGASGISGYY440 pKa = 10.82SGFSGEE446 pKa = 4.34VGASGDD452 pKa = 3.73SGFSGWSGEE461 pKa = 3.92IGASGISGYY470 pKa = 10.83SGISGFSGYY479 pKa = 10.11SGQIGASGFSGISGFSGFEE498 pKa = 3.95GASGHH503 pKa = 6.01SGYY506 pKa = 10.74SGWSGEE512 pKa = 4.17IGTSGHH518 pKa = 6.27SGLSGFSGFSGYY530 pKa = 10.77SGDD533 pKa = 4.08SGFSGYY539 pKa = 10.3SGEE542 pKa = 4.28SGYY545 pKa = 11.27SGAQGATGGSSSLFLYY561 pKa = 10.41KK562 pKa = 10.24ADD564 pKa = 3.61NTATSGEE571 pKa = 4.28PSPGHH576 pKa = 5.61VLWNNATQISATQINIDD593 pKa = 4.58HH594 pKa = 6.28LTEE597 pKa = 5.3DD598 pKa = 3.96NTDD601 pKa = 2.67IDD603 pKa = 3.6IFLAGLEE610 pKa = 4.09NTEE613 pKa = 3.94QFTIQDD619 pKa = 3.86RR620 pKa = 11.84NVSSNNQVWLVNGTPTNINPGTSNSYY646 pKa = 6.67WTVPVSLVSSNGTGTTGFANNQQLFLAIINGISGYY681 pKa = 10.58SGFSGYY687 pKa = 10.35SGYY690 pKa = 10.99SGAQGTSGFSGYY702 pKa = 10.28SGYY705 pKa = 11.03SGLQGNDD712 pKa = 2.9GFSGYY717 pKa = 10.11SGYY720 pKa = 10.7SGYY723 pKa = 10.85SGLVGDD729 pKa = 4.97SGYY732 pKa = 10.54SGHH735 pKa = 6.69SGISGFSGYY744 pKa = 10.11SGQVGQSGYY753 pKa = 10.26SGYY756 pKa = 10.41SGEE759 pKa = 4.22VGALGISGYY768 pKa = 10.16SGYY771 pKa = 10.54SGYY774 pKa = 10.85SGLVGDD780 pKa = 4.97SGYY783 pKa = 10.54SGHH786 pKa = 6.66SGTSGFSGWSGAVGQSGYY804 pKa = 10.63SGYY807 pKa = 10.99SGLQGNDD814 pKa = 3.11GLSGYY819 pKa = 9.79SGYY822 pKa = 10.68SGYY825 pKa = 10.85SGLVGDD831 pKa = 4.99SGYY834 pKa = 10.98SGYY837 pKa = 11.06SGFSGFSGEE846 pKa = 4.56KK847 pKa = 9.78GDD849 pKa = 4.14SGFSGFSGEE858 pKa = 4.05QGTPGLSGYY867 pKa = 9.96SGYY870 pKa = 10.98SGAQGPEE877 pKa = 3.74GHH879 pKa = 6.27SGYY882 pKa = 10.82SGINGASGISGFSGAQGEE900 pKa = 4.66SGISGYY906 pKa = 10.4SGYY909 pKa = 10.63SGEE912 pKa = 4.53QGTSGYY918 pKa = 10.79SGINGEE924 pKa = 4.46SGYY927 pKa = 10.62SGYY930 pKa = 10.79SGAVGASGISGYY942 pKa = 10.03SGWSGAVGISGYY954 pKa = 10.22SGYY957 pKa = 11.02SGIDD961 pKa = 3.32GANGSSGISGYY972 pKa = 10.41SGYY975 pKa = 11.04SGIDD979 pKa = 3.7GINGEE984 pKa = 4.58SGYY987 pKa = 10.72SGYY990 pKa = 10.92SGAEE994 pKa = 4.04GASGLSGYY1002 pKa = 10.03SGYY1005 pKa = 11.01SGAQGLSGYY1014 pKa = 10.48SGINGYY1020 pKa = 10.24SGISGFSGANGASGYY1035 pKa = 10.08SGYY1038 pKa = 10.9SGAQGISGYY1047 pKa = 10.13SGYY1050 pKa = 10.94SGATGPTVYY1059 pKa = 9.74PGAGMAVSTGSAWTTSKK1076 pKa = 9.85ATPTGVVVGDD1086 pKa = 4.13TDD1088 pKa = 4.02TQTLTNKK1095 pKa = 9.92RR1096 pKa = 11.84VTPRR1100 pKa = 11.84VLASTANSATPTLNTDD1116 pKa = 3.97LYY1118 pKa = 11.48DD1119 pKa = 3.45MMVITGQSVAITSFTTNLTGTPTNGQKK1146 pKa = 10.54LWISITGTGAIAITWGASFEE1166 pKa = 4.27ASTVALPTTTTSTNRR1181 pKa = 11.84LDD1183 pKa = 3.19IGFVYY1188 pKa = 10.58NVATSDD1194 pKa = 3.44WRR1196 pKa = 11.84CVAVAA1201 pKa = 4.46

Molecular weight: 116.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H4FS95|A0A2H4FS95_9CAUD Uncharacterized protein OS=Methylophilaceae phage P19250A OX=1913040 GN=P19250A_0052 PE=4 SV=1
MM1 pKa = 7.21IRR3 pKa = 11.84KK4 pKa = 9.72DD5 pKa = 3.25KK6 pKa = 10.85VRR8 pKa = 11.84NTEE11 pKa = 3.34IPLYY15 pKa = 9.53YY16 pKa = 9.83WIRR19 pKa = 11.84EE20 pKa = 3.98KK21 pKa = 11.37GSINNVKK28 pKa = 9.91SGMMLGYY35 pKa = 10.2RR36 pKa = 11.84PIGWHH41 pKa = 6.1IATEE45 pKa = 4.14VLEE48 pKa = 4.67RR49 pKa = 11.84YY50 pKa = 9.33YY51 pKa = 10.74KK52 pKa = 10.87LKK54 pKa = 10.67GIKK57 pKa = 8.53PNKK60 pKa = 8.22MNKK63 pKa = 9.07FDD65 pKa = 6.32KK66 pKa = 10.34IVCDD70 pKa = 3.25AAYY73 pKa = 9.58KK74 pKa = 10.52YY75 pKa = 10.6GSKK78 pKa = 10.05PQFVEE83 pKa = 4.14ANFNFHH89 pKa = 6.13VVGDD93 pKa = 4.0RR94 pKa = 11.84EE95 pKa = 4.12LL96 pKa = 4.9

Molecular weight: 11.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

58

0

58

12189

47

1201

210.2

23.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.892 ± 0.551

0.82 ± 0.127

5.128 ± 0.357

4.996 ± 0.553

4.291 ± 0.196

9.123 ± 1.885

1.362 ± 0.229

7.039 ± 0.283

5.53 ± 0.76

7.408 ± 0.511

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.322 ± 0.285

6.358 ± 0.412

3.643 ± 0.254

4.118 ± 0.327

3.191 ± 0.336

8.245 ± 1.388

7.351 ± 0.85

5.193 ± 0.266

1.493 ± 0.114

4.496 ± 0.365
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski